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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Desulfococcus oleovorans (strain DSM 6200 / Hxd3)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi99ZincUniRule annotation1
Metal bindingi101ZincUniRule annotation1
Metal bindingi126ZincUniRule annotation1
Metal bindingi128ZincUniRule annotation1
Binding sitei239ATPUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Dole_2533
OrganismiDesulfococcus oleovorans (strain DSM 6200 / Hxd3)
Taxonomic identifieri96561 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobacteraceaeDesulfococcus
Proteomesi
  • UP000008561 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003676631 – 467Glutamate--tRNA ligaseAdd BLAST467

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi96561.Dole_2533.

Structurei

3D structure databases

ProteinModelPortaliA8ZWA3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi10 – 20"HIGH" regionAdd BLAST11
Motifi236 – 240"KMSKS" region5

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252722.
KOiK01885.
OMAiAMGWEVP.
OrthoDBiPOG091H021W.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8ZWA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKQIITRFPP SPTGHLHIGG ARTALFNWLY ARHTGGRFIL RIEDTDVERS
60 70 80 90 100
TTQSAEGIIK SLEWLGIDWD EGPYFQSRRM EVYAEYIQRL LASGHAYYCT
110 120 130 140 150
CSPERLKERR ERALAEGRNP TYDGTCREKA LPPSDDAVVR FRTPDTGKTV
160 170 180 190 200
LDDRVKGGIA FDNAEIGDFI IQRSDQTPTY NFAVVVDDIT MGINTIIRGD
210 220 230 240 250
DHVTNTPRQI LMYRALDSEL PLFAHVPMVL GRDRSRLSKR HGAMSVLEYR
260 270 280 290 300
DTGYLPDGLI NALVRLGWSH GDQEFFTRKE LIELFSLEHI GTSAGVFDPD
310 320 330 340 350
KLLAINAEHI KKSDPAALAP HLLPLLKEKG YAAENNDYLH NAIHTLLLRS
360 370 380 390 400
KTLKEMADKA AFYYEDPLSY DPAATAKFLV PENMEILEML AEKLATLDSL
410 420 430 440 450
TEKDQEPAFT AVMEKTGKKF GKIAQPVRVA LTGRTESPGI FETIEALGRR
460
KTLERLADAV EMIKKST
Length:467
Mass (Da):52,586
Last modified:January 15, 2008 - v1
Checksum:iEC2171A4F6E65327
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000859 Genomic DNA. Translation: ABW68337.1.
RefSeqiWP_012175949.1. NC_009943.1.

Genome annotation databases

EnsemblBacteriaiABW68337; ABW68337; Dole_2533.
KEGGidol:Dole_2533.
PATRICi21695223. VBIDesOle35880_2607.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000859 Genomic DNA. Translation: ABW68337.1.
RefSeqiWP_012175949.1. NC_009943.1.

3D structure databases

ProteinModelPortaliA8ZWA3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi96561.Dole_2533.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABW68337; ABW68337; Dole_2533.
KEGGidol:Dole_2533.
PATRICi21695223. VBIDesOle35880_2607.

Phylogenomic databases

eggNOGiENOG4105C20. Bacteria.
COG0008. LUCA.
HOGENOMiHOG000252722.
KOiK01885.
OMAiAMGWEVP.
OrthoDBiPOG091H021W.

Family and domain databases

CDDicd00808. GluRS_core. 1 hit.
Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
1.10.8.70. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B. 1 hit.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020752. aa-tRNA-synth_I_codon-bd_sub1.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR033910. GluRS_core.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSYE_DESOH
AccessioniPrimary (citable) accession number: A8ZWA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: January 15, 2008
Last modified: November 30, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.