Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

A8ZWA3

- SYE_DESOH

UniProt

A8ZWA3 - SYE_DESOH

Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Desulfococcus oleovorans (strain DSM 6200 / Hxd3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (15 Jan 2008)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

    Catalytic activityi

    ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi99 – 991ZincUniRule annotation
    Metal bindingi101 – 1011ZincUniRule annotation
    Metal bindingi126 – 1261ZincUniRule annotation
    Metal bindingi128 – 1281ZincUniRule annotation
    Binding sitei239 – 2391ATPUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW
    4. tRNA binding Source: InterPro

    GO - Biological processi

    1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciDOLE96561:GHF3-2569-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
    Alternative name(s):
    Glutamyl-tRNA synthetaseUniRule annotation
    Short name:
    GluRSUniRule annotation
    Gene namesi
    Name:gltXUniRule annotation
    Ordered Locus Names:Dole_2533
    OrganismiDesulfococcus oleovorans (strain DSM 6200 / Hxd3)
    Taxonomic identifieri96561 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobacteraceaeDesulfococcus
    ProteomesiUP000008561: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 467467Glutamate--tRNA ligasePRO_0000367663Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi96561.Dole_2533.

    Structurei

    3D structure databases

    ProteinModelPortaliA8ZWA3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi10 – 2011"HIGH" regionAdd
    BLAST
    Motifi236 – 2405"KMSKS" region

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0008.
    HOGENOMiHOG000252722.
    KOiK01885.
    OMAiAMGWEVP.
    OrthoDBiEOG6DRPF7.

    Family and domain databases

    Gene3Di1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPiMF_00022_B. Glu_tRNA_synth_B.
    InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view]
    PANTHERiPTHR10119. PTHR10119. 1 hit.
    PfamiPF00749. tRNA-synt_1c. 1 hit.
    [Graphical view]
    PRINTSiPR00987. TRNASYNTHGLU.
    SUPFAMiSSF48163. SSF48163. 1 hit.
    TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8ZWA3-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKQIITRFPP SPTGHLHIGG ARTALFNWLY ARHTGGRFIL RIEDTDVERS    50
    TTQSAEGIIK SLEWLGIDWD EGPYFQSRRM EVYAEYIQRL LASGHAYYCT 100
    CSPERLKERR ERALAEGRNP TYDGTCREKA LPPSDDAVVR FRTPDTGKTV 150
    LDDRVKGGIA FDNAEIGDFI IQRSDQTPTY NFAVVVDDIT MGINTIIRGD 200
    DHVTNTPRQI LMYRALDSEL PLFAHVPMVL GRDRSRLSKR HGAMSVLEYR 250
    DTGYLPDGLI NALVRLGWSH GDQEFFTRKE LIELFSLEHI GTSAGVFDPD 300
    KLLAINAEHI KKSDPAALAP HLLPLLKEKG YAAENNDYLH NAIHTLLLRS 350
    KTLKEMADKA AFYYEDPLSY DPAATAKFLV PENMEILEML AEKLATLDSL 400
    TEKDQEPAFT AVMEKTGKKF GKIAQPVRVA LTGRTESPGI FETIEALGRR 450
    KTLERLADAV EMIKKST 467
    Length:467
    Mass (Da):52,586
    Last modified:January 15, 2008 - v1
    Checksum:iEC2171A4F6E65327
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000859 Genomic DNA. Translation: ABW68337.1.
    RefSeqiYP_001530414.1. NC_009943.1.

    Genome annotation databases

    EnsemblBacteriaiABW68337; ABW68337; Dole_2533.
    GeneIDi5695383.
    KEGGidol:Dole_2533.
    PATRICi21695223. VBIDesOle35880_2607.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000859 Genomic DNA. Translation: ABW68337.1 .
    RefSeqi YP_001530414.1. NC_009943.1.

    3D structure databases

    ProteinModelPortali A8ZWA3.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 96561.Dole_2533.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABW68337 ; ABW68337 ; Dole_2533 .
    GeneIDi 5695383.
    KEGGi dol:Dole_2533.
    PATRICi 21695223. VBIDesOle35880_2607.

    Phylogenomic databases

    eggNOGi COG0008.
    HOGENOMi HOG000252722.
    KOi K01885.
    OMAi AMGWEVP.
    OrthoDBi EOG6DRPF7.

    Enzyme and pathway databases

    BioCyci DOLE96561:GHF3-2569-MONOMER.

    Family and domain databases

    Gene3Di 1.10.10.350. 1 hit.
    1.10.1160.10. 1 hit.
    3.40.50.620. 2 hits.
    HAMAPi MF_00022_B. Glu_tRNA_synth_B.
    InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
    IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
    IPR001412. aa-tRNA-synth_I_CS.
    IPR004527. Glu-tRNA-ligase_bac/mito.
    IPR000924. Glu/Gln-tRNA-synth.
    IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
    IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
    IPR014729. Rossmann-like_a/b/a_fold.
    [Graphical view ]
    PANTHERi PTHR10119. PTHR10119. 1 hit.
    Pfami PF00749. tRNA-synt_1c. 1 hit.
    [Graphical view ]
    PRINTSi PR00987. TRNASYNTHGLU.
    SUPFAMi SSF48163. SSF48163. 1 hit.
    TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 6200 / Hxd3.

    Entry informationi

    Entry nameiSYE_DESOH
    AccessioniPrimary (citable) accession number: A8ZWA3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 24, 2009
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3