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A8ZWA3

- SYE_DESOH

UniProt

A8ZWA3 - SYE_DESOH

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Protein

Glutamate--tRNA ligase

Gene

gltX

Organism
Desulfococcus oleovorans (strain DSM 6200 / Hxd3)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu).UniRule annotation

Catalytic activityi

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi99 – 991ZincUniRule annotation
Metal bindingi101 – 1011ZincUniRule annotation
Metal bindingi126 – 1261ZincUniRule annotation
Metal bindingi128 – 1281ZincUniRule annotation
Binding sitei239 – 2391ATPUniRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. glutamate-tRNA ligase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW
  4. tRNA binding Source: InterPro

GO - Biological processi

  1. glutamyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciDOLE96561:GHF3-2569-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamate--tRNA ligaseUniRule annotation (EC:6.1.1.17UniRule annotation)
Alternative name(s):
Glutamyl-tRNA synthetaseUniRule annotation
Short name:
GluRSUniRule annotation
Gene namesi
Name:gltXUniRule annotation
Ordered Locus Names:Dole_2533
OrganismiDesulfococcus oleovorans (strain DSM 6200 / Hxd3)
Taxonomic identifieri96561 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobacteraceaeDesulfococcus
ProteomesiUP000008561: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 467467Glutamate--tRNA ligasePRO_0000367663Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi96561.Dole_2533.

Structurei

3D structure databases

ProteinModelPortaliA8ZWA3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi10 – 2011"HIGH" regionAdd
BLAST
Motifi236 – 2405"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0008.
HOGENOMiHOG000252722.
KOiK01885.
OMAiAMGWEVP.
OrthoDBiEOG6DRPF7.

Family and domain databases

Gene3Di1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPiMF_00022_B. Glu_tRNA_synth_B.
InterProiIPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PANTHERiPTHR10119. PTHR10119. 1 hit.
PfamiPF00749. tRNA-synt_1c. 1 hit.
[Graphical view]
PRINTSiPR00987. TRNASYNTHGLU.
SUPFAMiSSF48163. SSF48163. 1 hit.
TIGRFAMsiTIGR00464. gltX_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8ZWA3-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKQIITRFPP SPTGHLHIGG ARTALFNWLY ARHTGGRFIL RIEDTDVERS
60 70 80 90 100
TTQSAEGIIK SLEWLGIDWD EGPYFQSRRM EVYAEYIQRL LASGHAYYCT
110 120 130 140 150
CSPERLKERR ERALAEGRNP TYDGTCREKA LPPSDDAVVR FRTPDTGKTV
160 170 180 190 200
LDDRVKGGIA FDNAEIGDFI IQRSDQTPTY NFAVVVDDIT MGINTIIRGD
210 220 230 240 250
DHVTNTPRQI LMYRALDSEL PLFAHVPMVL GRDRSRLSKR HGAMSVLEYR
260 270 280 290 300
DTGYLPDGLI NALVRLGWSH GDQEFFTRKE LIELFSLEHI GTSAGVFDPD
310 320 330 340 350
KLLAINAEHI KKSDPAALAP HLLPLLKEKG YAAENNDYLH NAIHTLLLRS
360 370 380 390 400
KTLKEMADKA AFYYEDPLSY DPAATAKFLV PENMEILEML AEKLATLDSL
410 420 430 440 450
TEKDQEPAFT AVMEKTGKKF GKIAQPVRVA LTGRTESPGI FETIEALGRR
460
KTLERLADAV EMIKKST
Length:467
Mass (Da):52,586
Last modified:January 15, 2008 - v1
Checksum:iEC2171A4F6E65327
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000859 Genomic DNA. Translation: ABW68337.1.
RefSeqiYP_001530414.1. NC_009943.1.

Genome annotation databases

EnsemblBacteriaiABW68337; ABW68337; Dole_2533.
GeneIDi5695383.
KEGGidol:Dole_2533.
PATRICi21695223. VBIDesOle35880_2607.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000859 Genomic DNA. Translation: ABW68337.1 .
RefSeqi YP_001530414.1. NC_009943.1.

3D structure databases

ProteinModelPortali A8ZWA3.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 96561.Dole_2533.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABW68337 ; ABW68337 ; Dole_2533 .
GeneIDi 5695383.
KEGGi dol:Dole_2533.
PATRICi 21695223. VBIDesOle35880_2607.

Phylogenomic databases

eggNOGi COG0008.
HOGENOMi HOG000252722.
KOi K01885.
OMAi AMGWEVP.
OrthoDBi EOG6DRPF7.

Enzyme and pathway databases

BioCyci DOLE96561:GHF3-2569-MONOMER.

Family and domain databases

Gene3Di 1.10.10.350. 1 hit.
1.10.1160.10. 1 hit.
3.40.50.620. 2 hits.
HAMAPi MF_00022_B. Glu_tRNA_synth_B.
InterProi IPR008925. aa-tRNA-synth_I_codon-bd.
IPR020751. aa-tRNA-synth_I_codon-bd_sub2.
IPR001412. aa-tRNA-synth_I_CS.
IPR004527. Glu-tRNA-ligase_bac/mito.
IPR000924. Glu/Gln-tRNA-synth.
IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl.
IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view ]
PANTHERi PTHR10119. PTHR10119. 1 hit.
Pfami PF00749. tRNA-synt_1c. 1 hit.
[Graphical view ]
PRINTSi PR00987. TRNASYNTHGLU.
SUPFAMi SSF48163. SSF48163. 1 hit.
TIGRFAMsi TIGR00464. gltX_bact. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 6200 / Hxd3.

Entry informationi

Entry nameiSYE_DESOH
AccessioniPrimary (citable) accession number: A8ZWA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: January 15, 2008
Last modified: November 26, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3