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Protein

Hydroxyethylthiazole kinase

Gene

thiM

Organism
Desulfococcus oleovorans (strain DSM 6200 / Hxd3)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the hydroxyl group of 4-methyl-5-beta-hydroxyethylthiazole (THZ).UniRule annotation

Catalytic activityi

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Hydroxyethylthiazole kinase (thiM)
This subpathway is part of the pathway thiamine diphosphate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 4-methyl-5-(2-phosphoethyl)-thiazole from 5-(2-hydroxyethyl)-4-methylthiazole, the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei49Substrate; via amide nitrogenUniRule annotation1
Binding sitei125ATPUniRule annotation1
Binding sitei173ATPUniRule annotation1
Binding sitei200Substrate; via amide nitrogenUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processThiamine biosynthesis
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDOLE96561:G1GAC-785-MONOMER
UniPathwayiUPA00060; UER00139

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyethylthiazole kinaseUniRule annotation (EC:2.7.1.50UniRule annotation)
Alternative name(s):
4-methyl-5-beta-hydroxyethylthiazole kinaseUniRule annotation
Short name:
TH kinaseUniRule annotation
Short name:
Thz kinaseUniRule annotation
Gene namesi
Name:thiMUniRule annotation
Ordered Locus Names:Dole_0749
OrganismiDesulfococcus oleovorans (strain DSM 6200 / Hxd3)
Taxonomic identifieri96561 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobacteraceaeDesulfococcus
Proteomesi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003838511 – 274Hydroxyethylthiazole kinaseAdd BLAST274

Proteomic databases

PRIDEiA8ZV98

Interactioni

Protein-protein interaction databases

STRINGi96561.Dole_0749

Structurei

3D structure databases

ProteinModelPortaliA8ZV98
SMRiA8ZV98
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Thz kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4U Bacteria
COG2145 LUCA
HOGENOMiHOG000114352
KOiK00878
OMAiKRPLVHN
OrthoDBiPOG091H004S

Family and domain databases

CDDicd01170 THZ_kinase, 1 hit
Gene3Di3.40.1190.20, 1 hit
HAMAPiMF_00228 Thz_kinase, 1 hit
InterProiView protein in InterPro
IPR000417 Hyethyz_kinase
IPR029056 Ribokinase-like
PANTHERiPTHR44423 PTHR44423, 1 hit
PfamiView protein in Pfam
PF02110 HK, 1 hit
PIRSFiPIRSF000513 Thz_kinase, 1 hit
PRINTSiPR01099 HYETHTZKNASE
SUPFAMiSSF53613 SSF53613, 1 hit
TIGRFAMsiTIGR00694 thiM, 1 hit

Sequencei

Sequence statusi: Complete.

A8ZV98-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNEHEFAQKT IAIRRKVREQ RPLVHHITNF VVMNITANVT LAVGASPVMA
60 70 80 90 100
HAHEEVKEMA AFAGALNLNI GTLTPYWIDA MVMAGKVAEQ RGIPIVLDPV
110 120 130 140 150
GSGATPLRTG AAKKILAEVG VNVIRGNASE VMSLFADKET VNIRGVDSLE
160 170 180 190 200
TVDAVRHEAG LLAGELEKVV AVTGPVDIVT DGTRTLEVHN GTPMFGRVTG
210 220 230 240 250
TGCSATTVIS CFCAVEPDLL VASAAALGYY GLAGEEAARI SNGPGSFQAA
260 270
LLDTLYNLPE KIIQEKLRIR EVLP
Length:274
Mass (Da):29,060
Last modified:January 15, 2008 - v1
Checksum:i14C6D7D04ACA763D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000859 Genomic DNA Translation: ABW66559.1
RefSeqiWP_012174177.1, NC_009943.1

Genome annotation databases

EnsemblBacteriaiABW66559; ABW66559; Dole_0749
KEGGidol:Dole_0749

Similar proteinsi

Entry informationi

Entry nameiTHIM_DESOH
AccessioniPrimary (citable) accession number: A8ZV98
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: January 15, 2008
Last modified: May 23, 2018
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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