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A8ZUS9 (BIOB_DESOH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 45. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Biotin synthase

EC=2.8.1.6
Gene names
Name:bioB
Ordered Locus Names:Dole_0682
OrganismDesulfococcus oleovorans (strain DSM 6200 / Hxd3) [Complete proteome] [HAMAP]
Taxonomic identifier96561 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobacteraceaeDesulfococcus

Protein attributes

Sequence length329 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity. HAMAP-Rule MF_01694

Catalytic activity

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_01694

Cofactor

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathway

Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 2/2. HAMAP-Rule MF_01694

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01694

Sequence similarities

Belongs to the radical SAM superfamily. Biotin synthase family.

Sequence caution

The sequence ABW66492.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 329329Biotin synthase HAMAP-Rule MF_01694
PRO_0000381348

Sites

Metal binding681Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding721Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding751Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal binding1121Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding1431Iron-sulfur 2 (2Fe-2S) By similarity
Metal binding2031Iron-sulfur 2 (2Fe-2S) By similarity

Sequences

Sequence LengthMass (Da)Tools
A8ZUS9 [UniParc].

Last modified July 28, 2009. Version 2.
Checksum: 0F196227C053809C

FASTA32935,358
        10         20         30         40         50         60 
MLHPIQLTDT ARRIQAGETP DDDTLLSLIR MPDDRTFLLF PGADLLREHF FGTSVHLCVI 

        70         80         90        100        110        120 
RNGKSGRCSE DCAFCSQSVR AKADIDAYPL ISEIDLAEPG RRLESTPVNR YSVVTSGRGL 

       130        140        150        160        170        180 
SSAEVSTVSR ALAAIDTTAV STCASLGILD PGQLAMLRDA GVTRYHHNLE SAESLFANVC 

       190        200        210        220        230        240 
TTHTYEERVQ TVKAAKAAGL SVCCGGIFGL GETDDQVAEL AFALRDLDVD SVPVNFLVPI 

       250        260        270        280        290        300 
KGTAMEKASR LTPLRCLRII AFLRHVLPDK EIIVCGGRVH NLGDLHPLVF YAGASGIMTG 

       310        320 
DYLTTSGRNL ADDLALLATL GMTPREKAG 

« Hide

References

[1]"Complete sequence of Desulfococcus oleovorans Hxd3."
US DOE Joint Genome Institute
Copeland A., Lucas S., Lapidus A., Barry K., Glavina del Rio T., Dalin E., Tice H., Pitluck S., Kiss H., Brettin T., Bruce D., Detter J.C., Han C., Schmutz J., Larimer F., Land M., Hauser L., Kyrpides N. expand/collapse author list , Kim E., Wawrik B., Richardson P.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 6200 / Hxd3.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000859 Genomic DNA. Translation: ABW66492.1. Different initiation.
RefSeqYP_001528569.1. NC_009943.1.

3D structure databases

ProteinModelPortalA8ZUS9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING96561.Dole_0682.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABW66492; ABW66492; Dole_0682.
GeneID5693512.
KEGGdol:Dole_0682.
PATRIC21691365. VBIDesOle35880_0699.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0502.
HOGENOMHOG000239958.
KOK01012.
OrthoDBEOG622PMP.

Enzyme and pathway databases

BioCycDOLE96561:GHF3-698-MONOMER.
UniPathwayUPA00078; UER00162.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01694. BioB.
InterProIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF001619. Biotin_synth. 1 hit.
SMARTSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00433. bioB. 1 hit.
ProtoNetSearch...

Entry information

Entry nameBIOB_DESOH
AccessionPrimary (citable) accession number: A8ZUS9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: July 28, 2009
Last modified: May 14, 2014
This is version 45 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways