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Protein

Glutamyl-tRNA(Gln) amidotransferase subunit A

Gene

gatA

Organism
Desulfococcus oleovorans (strain DSM 6200 / Hxd3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Allows the formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu-tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activated gamma-phospho-Glu-tRNA(Gln).UniRule annotation

Catalytic activityi

ATP + L-glutamyl-tRNA(Gln) + L-glutamine = ADP + phosphate + L-glutaminyl-tRNA(Gln) + L-glutamate.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei78 – 781Charge relay systemUniRule annotation
Active sitei153 – 1531Charge relay systemUniRule annotation
Active sitei177 – 1771Acyl-ester intermediateUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciDOLE96561:GHF3-152-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Glutamyl-tRNA(Gln) amidotransferase subunit AUniRule annotation (EC:6.3.5.7UniRule annotation)
Short name:
Glu-ADT subunit AUniRule annotation
Gene namesi
Name:gatAUniRule annotation
Ordered Locus Names:Dole_0151
OrganismiDesulfococcus oleovorans (strain DSM 6200 / Hxd3)
Taxonomic identifieri96561 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobacteraceaeDesulfococcus
Proteomesi
  • UP000008561 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 486486Glutamyl-tRNA(Gln) amidotransferase subunit APRO_1000095132Add
BLAST

Interactioni

Subunit structurei

Heterotrimer of A, B and C subunits.UniRule annotation

Protein-protein interaction databases

STRINGi96561.Dole_0151.

Structurei

3D structure databases

ProteinModelPortaliA8ZSP8.
SMRiA8ZSP8. Positions 1-486.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the amidase family. GatA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiPOG091H00O9.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8ZSP8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEPYALTIHE ARQLLDKKEI SSVELTRSVF DRIERVEPKV GAYITLARES
60 70 80 90 100
ALSAAAAADT RLQSGGIAPL TGIPLAVKDL ICTTGLRTTC ASRMLETFMP
110 120 130 140 150
PYDATVIEKL KTQGAVITGK VNMDEFAMGS STEHSALGST KNPWNLACIP
160 170 180 190 200
GGSSGGSAAS VAADMCLGSL GSDTGGSIRQ PASHCGVVGI KPTYGRVSRY
210 220 230 240 250
GLVAFASSLD QIGVLAKDVT DSALLLSAIS GHDPSDSTSV PREVPDYAAS
260 270 280 290 300
CEKGLSGLTI GVCREYINAE GLDPEVADTV NRSIEHMKAQ GAACVEVSLP
310 320 330 340 350
HAQYAVAAYY VIAPAEASSN LARYDGVKYG FRDPEADSLL EMYARTRSRG
360 370 380 390 400
FGKEVQRRII IGTYCLSSGY YDAYYRKASQ VRSLIADDFK KAFETCDVIV
410 420 430 440 450
SPVAPTPAFE LGAKTEDPLT MYLSDIFTLS ANLAGVPGMS VPGGFSSTGL
460 470 480
PIGVQLIAGH FNEPALVRCA WNLEQAVNIA NKKPAL
Length:486
Mass (Da):51,404
Last modified:January 15, 2008 - v1
Checksum:i8D294D053CB8598E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000859 Genomic DNA. Translation: ABW65961.1.
RefSeqiWP_012173580.1. NC_009943.1.

Genome annotation databases

EnsemblBacteriaiABW65961; ABW65961; Dole_0151.
KEGGidol:Dole_0151.
PATRICi21690251. VBIDesOle35880_0156.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000859 Genomic DNA. Translation: ABW65961.1.
RefSeqiWP_012173580.1. NC_009943.1.

3D structure databases

ProteinModelPortaliA8ZSP8.
SMRiA8ZSP8. Positions 1-486.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi96561.Dole_0151.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABW65961; ABW65961; Dole_0151.
KEGGidol:Dole_0151.
PATRICi21690251. VBIDesOle35880_0156.

Phylogenomic databases

eggNOGiENOG4105C3P. Bacteria.
COG0154. LUCA.
HOGENOMiHOG000116699.
KOiK02433.
OMAiKEYFGAG.
OrthoDBiPOG091H00O9.

Enzyme and pathway databases

BioCyciDOLE96561:GHF3-152-MONOMER.

Family and domain databases

Gene3Di3.90.1300.10. 1 hit.
HAMAPiMF_00120. GatA. 1 hit.
InterProiIPR000120. Amidase.
IPR020556. Amidase_CS.
IPR023631. Amidase_dom.
IPR004412. GatA.
[Graphical view]
PANTHERiPTHR11895. PTHR11895. 1 hit.
PfamiPF01425. Amidase. 1 hit.
[Graphical view]
SUPFAMiSSF75304. SSF75304. 1 hit.
TIGRFAMsiTIGR00132. gatA. 1 hit.
PROSITEiPS00571. AMIDASES. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiGATA_DESOH
AccessioniPrimary (citable) accession number: A8ZSP8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: January 15, 2008
Last modified: September 7, 2016
This is version 56 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.