Reviewed, UniProtKB/Swiss-Prot A8ZQ66 (SYM1_ACAM1)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase 1
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase 1
      Short name=MetRS 1

Gene names

Name:	metG1
Ordered Locus Names:	AM1_F0085

Encoded on

Plasmid pREB6

Organism

Acaryochloris marina (strain MBIC 11017) [Complete proteome] [HAMAP]

Taxonomic identifier

329726 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Acaryochloris

Protein attributes

Sequence length	558 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_00098
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Plasmid
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 558	558	Methionyl-tRNA synthetase 1 HAMAP MF_00098		PRO_0000331770
Regions
	Motif	10 – 20	11	"HIGH" region HAMAP MF_00098
	Motif	332 – 336	5	"KMSKS" region HAMAP MF_00098
Sites
	Metal binding	142	1	Zinc By similarity
	Metal binding	145	1	Zinc By similarity
	Metal binding	155	1	Zinc By similarity
	Metal binding	158	1	Zinc By similarity
	Binding site	335	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8ZQ66-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: A6481D3ED2FF1293	FASTA	558	64,196
	10 20 30 40 50 60 MRYLITSALP YINGIKHLGN LVGSMLPADI YARFLRQEGE EVLYICATDE HGTPAEIAAI 70 80 90 100 110 120 DAGLEVAEFC AKQYHKQKEI YKRFGLSFDY FGRTSAPENH ELTQYFYQQL AKQNFIEERE 130 140 150 160 170 180 ISQFYALDDQ RFLPDRYVTG TCPHCGYEQA RGDQCENCTK VLTPTELIKP RSTISGSTHL 190 200 210 220 230 240 ELRTSRHLFL RLDKLSDEVR NWVDKQTQWS TLTKSIALKW LNEGLKSRCI TRDLVWGVPV 250 260 270 280 290 300 PTEGFERKVF YVWFDAPIGY ISATKAWGDI TNNDWECWWK ESDDVHYTQF MAKDNLPFHT 310 320 330 340 350 360 IMWPATILGS REPWKMVDYI KGFNWLNYYG GKFSTSSQRG VFLDQALEIA SADNWRYMLI 370 380 390 400 410 420 ANAPESADSA FTWEQFQKQV NKELADNLGN FVNRILKFTA SRFGMTLPEG GTPGDAEAEL 430 440 450 460 470 480 QVTCNELVEK LRKYLHNLEF RRATETLNAL WRTGNQYIDV RAPWVLFKTD QDETAMVIRT 490 500 510 520 530 540 CVNLIRLYAI SSAPFIPHTT QALFDALQLT DVERRHTMTE ASDLTLLAAG RSFMVPAPLF 550 QKIDDDLVAE LKAQYGGE

References

[1]

"Niche adaptation and genome expansion in the chlorophyll d-producing cyanobacterium Acaryochloris marina." Swingley W.D., Chen M., Cheung P.C., Conrad A.L., Dejesa L.C., Hao J., Honchak B.M., Karbach L.E., Kurdoglu A., Lahiri S., Mastrian S.D., Miyashita H., Page L., Ramakrishna P., Satoh S., Sattley W.M., Shimada Y., Taylor H.L. , Tomo T., Tsuchiya T., Wang Z.T., Raymond J., Mimuro M., Blankenship R.E., Touchman J.W. Proc. Natl. Acad. Sci. U.S.A. 105:2005-2010(2008) [PubMed: 18252824] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000843 Genomic DNA. Translation: ABW33099.1.
RefSeq	YP_001522389.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5687006.
GenomeReviews	Gene locus AM1_F0085 in contig CP000843_GR.
KEGG	amr:AM1_F0085.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8ZQ66. YINGIKH.
Family and domain databases
HAMAP	MF_00098. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR001412. aa-tRNA-synth_I_CS. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
TIGRFAMs	TIGR00398. metG. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM1_ACAM1

Accession

Primary (citable) accession number: A8ZQ66

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 29, 2008
Last sequence update:	January 15, 2008
Last modified:	June 16, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)