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A8Z6P6

- SYI_CAMC1

UniProt

A8Z6P6 - SYI_CAMC1

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Campylobacter concisus (strain 13826)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 46 (01 Oct 2014)
      Sequence version 1 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei570 – 5701Aminoacyl-adenylateUniRule annotation
    Binding sitei614 – 6141ATPUniRule annotation
    Metal bindingi893 – 8931ZincUniRule annotation
    Metal bindingi896 – 8961ZincUniRule annotation
    Metal bindingi908 – 9081ZincUniRule annotation
    Metal bindingi911 – 9111ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCCON360104:GHAC-1760-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:Ccon26_16660
    ORF Names:CCC13826_0278
    OrganismiCampylobacter concisus (strain 13826)
    Taxonomic identifieri360104 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
    ProteomesiUP000001121: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 918918Isoleucine--tRNA ligasePRO_1000073707Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Structurei

    3D structure databases

    ProteinModelPortaliA8Z6P6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi59 – 6911"HIGH" regionAdd
    BLAST
    Motifi611 – 6155"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    OMAiERLMLHQ.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8Z6P6-1 [UniParc]FASTAAdd to Basket

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    MDYKETLLLP ETNFPMRGNL PQNEPQRLKS WYEERKVYEK MKKNRQNAVK    50
    NFNIHDGPPY ANGHLHIGHA LNKILKDIIT KTHYFYGENV RYVPGWDCHG 100
    LPIEQQVEVK LGDKKKELSK VEIRELCRQH AREFIDIQRD EFKTLGIIGD 150
    FENPYMTMKF EFEADIYKAL CEIAKKGLLV ERSKPVYWSW AARSALAEAE 200
    VEYEEKEDYS IYVAFELDGD ALEKLGVKEA SAVIWTTTPW TLPANQAISL 250
    KPDEIYVLTA ENLIFAKPLL ESVVQSGLSK GEIKKEFKSS LLENTHAINP 300
    LNGRKSKFLL GDHVMMDGGT GLVHTAPGHG EDDYYVCLKY GFSEILMPVD 350
    DGGCYDESIK HHGLFRSDVV DEFVGMHIFK ANEKILELLG KSLLSVSKFR 400
    HSYPFCWRTH KPVIYRATKQ WFIAMDESKL GGKTLRQTAL KELEKVKFYP 450
    SVGIKRIGSM IENRPDWCIS RQRDWGVPIA FFRDKATKEV IFDSEILDHI 500
    AGIFKEKGAD AWWALSIDEL LPKGSKYKAE NLEKVMDILD VWFDSGSTWH 550
    AVLQSDNYDA GKYPASMYLE GSDQHRGWFQ SSLLVSTAIN SHAPYESILT 600
    HGFTVDAKGE KMSKSKGNVI APQDVAKTHG VEILRLWVGM SDYSSDLKIS 650
    EDILKQISEQ YRKIRNTIRF LLANVNDLES LNTEFNILDK WILARAKKVF 700
    DEASACFKNY DFSKGFNILL NFLSADLSGV YLDVCKDRLY CDAKDAPRRR 750
    SAQSAMAIIT KTLLPLIAPT LTYTVDEVMD YAPKIIKGEA KDAFDLVYEP 800
    IKFDLSFEDE LLFASREKFN EIVDVLKKDK KIKSTLELSL ETTNHNITSY 850
    DEREVADLYM VSSVRAYDDS EPLAEFELEG DKFKIIASNL HKCPRCWKFN 900
    ASKEDALCPR CEEVISAK 918
    Length:918
    Mass (Da):105,052
    Last modified:January 15, 2008 - v1
    Checksum:i56BA720A42206B62
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000792 Genomic DNA. Translation: ABW74825.1.
    RefSeqiWP_012140300.1. NC_009802.1.
    YP_004777023.1. NC_009802.1.

    Genome annotation databases

    EnsemblBacteriaiABW74825; ABW74825; CCC13826_0278.
    GeneIDi5597249.
    KEGGicco:CCC13826_0278.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000792 Genomic DNA. Translation: ABW74825.1 .
    RefSeqi WP_012140300.1. NC_009802.1.
    YP_004777023.1. NC_009802.1.

    3D structure databases

    ProteinModelPortali A8Z6P6.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABW74825 ; ABW74825 ; CCC13826_0278 .
    GeneIDi 5597249.
    KEGGi cco:CCC13826_0278.

    Phylogenomic databases

    OMAi ERLMLHQ.

    Enzyme and pathway databases

    BioCyci CCON360104:GHAC-1760-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of Campylobacter concisus 13826 isolated from human feces."
      Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., On S., Nelson K.E.
      Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: 13826.

    Entry informationi

    Entry nameiSYI_CAMC1
    AccessioniPrimary (citable) accession number: A8Z6P6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 46 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3