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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Campylobacter concisus (strain 13826)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei570 – 5701Aminoacyl-adenylateUniRule annotation
Binding sitei614 – 6141ATPUniRule annotation
Metal bindingi893 – 8931ZincUniRule annotation
Metal bindingi896 – 8961ZincUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCCON360104:GHAC-1760-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:Ccon26_16660
ORF Names:CCC13826_0278
OrganismiCampylobacter concisus (strain 13826)
Taxonomic identifieri360104 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000001121 Componenti: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 918918Isoleucine--tRNA ligasePRO_1000073707Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA8Z6P6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionAdd
BLAST
Motifi611 – 6155"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

OMAiVLGDWDN.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8Z6P6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYKETLLLP ETNFPMRGNL PQNEPQRLKS WYEERKVYEK MKKNRQNAVK
60 70 80 90 100
NFNIHDGPPY ANGHLHIGHA LNKILKDIIT KTHYFYGENV RYVPGWDCHG
110 120 130 140 150
LPIEQQVEVK LGDKKKELSK VEIRELCRQH AREFIDIQRD EFKTLGIIGD
160 170 180 190 200
FENPYMTMKF EFEADIYKAL CEIAKKGLLV ERSKPVYWSW AARSALAEAE
210 220 230 240 250
VEYEEKEDYS IYVAFELDGD ALEKLGVKEA SAVIWTTTPW TLPANQAISL
260 270 280 290 300
KPDEIYVLTA ENLIFAKPLL ESVVQSGLSK GEIKKEFKSS LLENTHAINP
310 320 330 340 350
LNGRKSKFLL GDHVMMDGGT GLVHTAPGHG EDDYYVCLKY GFSEILMPVD
360 370 380 390 400
DGGCYDESIK HHGLFRSDVV DEFVGMHIFK ANEKILELLG KSLLSVSKFR
410 420 430 440 450
HSYPFCWRTH KPVIYRATKQ WFIAMDESKL GGKTLRQTAL KELEKVKFYP
460 470 480 490 500
SVGIKRIGSM IENRPDWCIS RQRDWGVPIA FFRDKATKEV IFDSEILDHI
510 520 530 540 550
AGIFKEKGAD AWWALSIDEL LPKGSKYKAE NLEKVMDILD VWFDSGSTWH
560 570 580 590 600
AVLQSDNYDA GKYPASMYLE GSDQHRGWFQ SSLLVSTAIN SHAPYESILT
610 620 630 640 650
HGFTVDAKGE KMSKSKGNVI APQDVAKTHG VEILRLWVGM SDYSSDLKIS
660 670 680 690 700
EDILKQISEQ YRKIRNTIRF LLANVNDLES LNTEFNILDK WILARAKKVF
710 720 730 740 750
DEASACFKNY DFSKGFNILL NFLSADLSGV YLDVCKDRLY CDAKDAPRRR
760 770 780 790 800
SAQSAMAIIT KTLLPLIAPT LTYTVDEVMD YAPKIIKGEA KDAFDLVYEP
810 820 830 840 850
IKFDLSFEDE LLFASREKFN EIVDVLKKDK KIKSTLELSL ETTNHNITSY
860 870 880 890 900
DEREVADLYM VSSVRAYDDS EPLAEFELEG DKFKIIASNL HKCPRCWKFN
910
ASKEDALCPR CEEVISAK
Length:918
Mass (Da):105,052
Last modified:January 15, 2008 - v1
Checksum:i56BA720A42206B62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000792 Genomic DNA. Translation: ABW74825.1.
RefSeqiYP_004777023.1. NC_009802.1.

Genome annotation databases

EnsemblBacteriaiABW74825; ABW74825; CCC13826_0278.
GeneIDi5597249.
KEGGicco:CCC13826_0278.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000792 Genomic DNA. Translation: ABW74825.1.
RefSeqiYP_004777023.1. NC_009802.1.

3D structure databases

ProteinModelPortaliA8Z6P6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABW74825; ABW74825; CCC13826_0278.
GeneIDi5597249.
KEGGicco:CCC13826_0278.

Phylogenomic databases

OMAiVLGDWDN.

Enzyme and pathway databases

BioCyciCCON360104:GHAC-1760-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Campylobacter concisus 13826 isolated from human feces."
    Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., On S., Nelson K.E.
    Submitted (SEP-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13826.

Entry informationi

Entry nameiSYI_CAMC1
AccessioniPrimary (citable) accession number: A8Z6P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 15, 2008
Last modified: April 1, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.