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A8Z6P6

- SYI_CAMC1

UniProt

A8Z6P6 - SYI_CAMC1

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Protein
Isoleucine--tRNA ligase
Gene
ileS, Ccon26_16660, CCC13826_0278
Organism
Campylobacter concisus (strain 13826)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei570 – 5701Aminoacyl-adenylate By similarity
Binding sitei614 – 6141ATP By similarity
Metal bindingi893 – 8931Zinc By similarity
Metal bindingi896 – 8961Zinc By similarity
Metal bindingi908 – 9081Zinc By similarity
Metal bindingi911 – 9111Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCCON360104:GHAC-1760-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:Ccon26_16660
ORF Names:CCC13826_0278
OrganismiCampylobacter concisus (strain 13826)
Taxonomic identifieri360104 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaEpsilonproteobacteriaCampylobacteralesCampylobacteraceaeCampylobacter
ProteomesiUP000001121: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 918918Isoleucine--tRNA ligaseUniRule annotation
PRO_1000073707Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA8Z6P6.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi59 – 6911"HIGH" regionUniRule annotation
Add
BLAST
Motifi611 – 6155"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

OMAiERLMLHQ.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8Z6P6-1 [UniParc]FASTAAdd to Basket

« Hide

MDYKETLLLP ETNFPMRGNL PQNEPQRLKS WYEERKVYEK MKKNRQNAVK    50
NFNIHDGPPY ANGHLHIGHA LNKILKDIIT KTHYFYGENV RYVPGWDCHG 100
LPIEQQVEVK LGDKKKELSK VEIRELCRQH AREFIDIQRD EFKTLGIIGD 150
FENPYMTMKF EFEADIYKAL CEIAKKGLLV ERSKPVYWSW AARSALAEAE 200
VEYEEKEDYS IYVAFELDGD ALEKLGVKEA SAVIWTTTPW TLPANQAISL 250
KPDEIYVLTA ENLIFAKPLL ESVVQSGLSK GEIKKEFKSS LLENTHAINP 300
LNGRKSKFLL GDHVMMDGGT GLVHTAPGHG EDDYYVCLKY GFSEILMPVD 350
DGGCYDESIK HHGLFRSDVV DEFVGMHIFK ANEKILELLG KSLLSVSKFR 400
HSYPFCWRTH KPVIYRATKQ WFIAMDESKL GGKTLRQTAL KELEKVKFYP 450
SVGIKRIGSM IENRPDWCIS RQRDWGVPIA FFRDKATKEV IFDSEILDHI 500
AGIFKEKGAD AWWALSIDEL LPKGSKYKAE NLEKVMDILD VWFDSGSTWH 550
AVLQSDNYDA GKYPASMYLE GSDQHRGWFQ SSLLVSTAIN SHAPYESILT 600
HGFTVDAKGE KMSKSKGNVI APQDVAKTHG VEILRLWVGM SDYSSDLKIS 650
EDILKQISEQ YRKIRNTIRF LLANVNDLES LNTEFNILDK WILARAKKVF 700
DEASACFKNY DFSKGFNILL NFLSADLSGV YLDVCKDRLY CDAKDAPRRR 750
SAQSAMAIIT KTLLPLIAPT LTYTVDEVMD YAPKIIKGEA KDAFDLVYEP 800
IKFDLSFEDE LLFASREKFN EIVDVLKKDK KIKSTLELSL ETTNHNITSY 850
DEREVADLYM VSSVRAYDDS EPLAEFELEG DKFKIIASNL HKCPRCWKFN 900
ASKEDALCPR CEEVISAK 918
Length:918
Mass (Da):105,052
Last modified:January 15, 2008 - v1
Checksum:i56BA720A42206B62
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000792 Genomic DNA. Translation: ABW74825.1.
RefSeqiWP_012140300.1. NC_009802.1.
YP_004777023.1. NC_009802.1.

Genome annotation databases

EnsemblBacteriaiABW74825; ABW74825; CCC13826_0278.
GeneIDi5597249.
KEGGicco:CCC13826_0278.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000792 Genomic DNA. Translation: ABW74825.1 .
RefSeqi WP_012140300.1. NC_009802.1.
YP_004777023.1. NC_009802.1.

3D structure databases

ProteinModelPortali A8Z6P6.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABW74825 ; ABW74825 ; CCC13826_0278 .
GeneIDi 5597249.
KEGGi cco:CCC13826_0278.

Phylogenomic databases

OMAi ERLMLHQ.

Enzyme and pathway databases

BioCyci CCON360104:GHAC-1760-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Campylobacter concisus 13826 isolated from human feces."
    Fouts D.E., Mongodin E.F., Puiu D., Sebastian Y., Miller W.G., Mandrell R.E., On S., Nelson K.E.
    Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 13826.

Entry informationi

Entry nameiSYI_CAMC1
AccessioniPrimary (citable) accession number: A8Z6P6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 15, 2008
Last modified: September 3, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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