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Protein

Chaperone protein DnaJ

Gene

dnaJ

Organism
Sulcia muelleri (strain GWSS)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 2 Zn2+ ions per monomer.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi156Zinc 2UniRule annotation1
Metal bindingi159Zinc 2UniRule annotation1
Metal bindingi182Zinc 2UniRule annotation1
Metal bindingi185Zinc 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri120 – 208CR-typeUniRule annotationAdd BLAST89

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

ChaperoneUniRule annotation

Keywords - Biological processi

DNA replicationUniRule annotation, Stress responseUniRule annotation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Chaperone protein DnaJUniRule annotation
Gene namesi
Name:dnaJUniRule annotationImported
Ordered Locus Names:SMGWSS_101Imported
OrganismiSulcia muelleri (strain GWSS)Imported
Taxonomic identifieri444179 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesFlavobacteriiaFlavobacterialesCandidatus Sulcia
Proteomesi
  • UP000000781 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliA8Z5W5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini5 – 69JInterPro annotationAdd BLAST65

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi76 – 118Gly-richUniRule annotationAdd BLAST43

Domaini

The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity.UniRule annotation

Sequence similaritiesi

Belongs to the DnaJ family.UniRule annotation
Contains 1 CR-type zinc finger.UniRule annotation
Contains 1 J domain.UniRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri120 – 208CR-typeUniRule annotationAdd BLAST89

Keywords - Domaini

RepeatUniRule annotation, Zinc-fingerUniRule annotation

Phylogenomic databases

HOGENOMiHOG000226717.
KOiK03686.
OMAiMKEDYYE.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8Z5W5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIKKDYYEIL GISRDASTDE IKKAYRKLAI KYHPDKNKEK QAEEKFKEAA
60 70 80 90 100
EAYDILSNPE KKKRYDQFGH SSSQGDSGGM NMEDIFTNFG DIFGDAFTFN
110 120 130 140 150
GSFSGFGFSN NKKKNIKGSD LRIRVKLTLN EILKGIEKKI KVQRMKIAKG
160 170 180 190 200
VEFIFCKNCN GTGQITRVTN TILGIMHTTT TCSICGGLGK KSERLPKGVN
210 220 230 240 250
NKGLKKKEEL VSITIPPGVT EGIQLKISGK GNDAPFGNGI PGDLIVVIEE
260 270 280 290 300
IPHDQLKREG KNLHYDLYIS FPDAVLGAYE EVPTGDGKAR LKIDAGTQSG
310 320 330 340 350
KTLRLKGKGL PSLEGYGRGD LLIHINVWTP KKLNEEQKKI FKQMKNEENF
360 370
IPSPQKYEKS FFEKVREMFS SER
Length:373
Mass (Da):41,819
Last modified:January 15, 2008 - v1
Checksum:i826D3D6A3736FEA5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000770 Genomic DNA. Translation: ABS30516.1.

Genome annotation databases

EnsemblBacteriaiABS30516; ABS30516; SMGWSS_101.
KEGGismg:SMGWSS_101.
PATRICi32018901. VBICanSul12747_0081.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000770 Genomic DNA. Translation: ABS30516.1.

3D structure databases

ProteinModelPortaliA8Z5W5.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABS30516; ABS30516; SMGWSS_101.
KEGGismg:SMGWSS_101.
PATRICi32018901. VBICanSul12747_0081.

Phylogenomic databases

HOGENOMiHOG000226717.
KOiK03686.
OMAiMKEDYYE.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
2.10.230.10. 1 hit.
HAMAPiMF_01152. DnaJ. 1 hit.
InterProiIPR012724. DnaJ.
IPR002939. DnaJ_C.
IPR001623. DnaJ_domain.
IPR018253. DnaJ_domain_CS.
IPR008971. HSP40/DnaJ_pept-bd.
IPR001305. HSP_DnaJ_Cys-rich_dom.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF01556. DnaJ_C. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
SSF49493. SSF49493. 3 hits.
SSF57938. SSF57938. 1 hit.
PROSITEiPS00636. DNAJ_1. 1 hit.
PS50076. DNAJ_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiA8Z5W5_SULMW
AccessioniPrimary (citable) accession number: A8Z5W5
Entry historyi
Integrated into UniProtKB/TrEMBL: January 15, 2008
Last sequence update: January 15, 2008
Last modified: November 30, 2016
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.