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Protein

Phosphoenolpyruvate carboxykinase [ATP]

Gene

pckA

Organism
Staphylococcus aureus (strain USA300 / TCH1516)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the gluconeogenesis. Catalyzes the conversion of oxaloacetate (OAA) to phosphoenolpyruvate (PEP) through direct phosphoryl transfer between the nucleoside triphosphate and OAA.UniRule annotation

Catalytic activityi

ATP + oxaloacetate = ADP + phosphoenolpyruvate + CO2.UniRule annotation

Cofactori

Mn2+UniRule annotationNote: Binds 1 Mn2+ ion per subunit.UniRule annotation

Pathwayi: gluconeogenesis

This protein is involved in the pathway gluconeogenesis, which is part of Carbohydrate biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway gluconeogenesis and in Carbohydrate biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei58SubstrateUniRule annotation1
Binding sitei195SubstrateUniRule annotation1
Metal bindingi201ManganeseUniRule annotation1
Binding sitei201ATPUniRule annotation1
Binding sitei201SubstrateUniRule annotation1
Metal bindingi220Manganese; via tele nitrogenUniRule annotation1
Binding sitei220ATPUniRule annotation1
Metal bindingi257ManganeseUniRule annotation1
Binding sitei285ATPUniRule annotation1
Binding sitei321ATPUniRule annotation1
Binding sitei321SubstrateUniRule annotation1
Binding sitei446ATPUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi236 – 244ATPUniRule annotation9
Nucleotide bindingi440 – 441ATPUniRule annotation2

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Gluconeogenesis

Keywords - Ligandi

ATP-binding, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00138.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoenolpyruvate carboxykinase [ATP]UniRule annotation (EC:4.1.1.49UniRule annotation)
Short name:
PCKUniRule annotation
Short name:
PEP carboxykinaseUniRule annotation
Short name:
PEPCKUniRule annotation
Gene namesi
Name:pckAUniRule annotation
Ordered Locus Names:USA300HOU_1778
OrganismiStaphylococcus aureus (strain USA300 / TCH1516)
Taxonomic identifieri451516 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000810071 – 530Phosphoenolpyruvate carboxykinase [ATP]Add BLAST530

Structurei

3D structure databases

ProteinModelPortaliA8Z4L3.
SMRiA8Z4L3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoenolpyruvate carboxykinase [ATP] family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.

Family and domain databases

CDDicd00484. PEPCK_ATP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8Z4L3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVDTYTETT KIDKLLKKPT SHFQLSTTQL YNKILDNNEG VLTELGAVNA
60 70 80 90 100
STGKYTGRSP KDKFFVSEPS YRDNIDWGEI NQPIDEETFL KLYHKVLDYL
110 120 130 140 150
DKKDELYVFK GYAGSDKDTM LKLTVINELA WHNLFAKNMF IRPESKEEAT
160 170 180 190 200
KIKPNFTIVS APHFKADPEV DGTKSETFVI ISFKHKVILI GGTEYAGEMK
210 220 230 240 250
KGIFSVMNYL LPMQDIMSMH CSANVGEKGD VALFFGLSGT GKTTLSADPH
260 270 280 290 300
RKLIGDDEHG WNKNGVFNIE GGCYAKAINL SKEKEPQIFD AIKYGAILEN
310 320 330 340 350
TVVAEDGSVD FEDNRYTENT RAAYPINHID NIVVPSKAAH PNTIIFLTAD
360 370 380 390 400
AFGVIPPISK LNKDQAMYHF LSGFTSKLAG TERGVTEPEP SFSTCFGAPF
410 420 430 440 450
FPLHPTVYAD LLGELIDLHD VDVYLVNTGW TGGKYGVGRR ISLHYTRQMV
460 470 480 490 500
NQAISGKLKN AEYTKDSTFG LSIPVEIEDV PKTILNPINA WSDKEKYKAQ
510 520 530
AEDLIQRFEK NFEKFGEKVE HIAEKGSFNK
Length:530
Mass (Da):59,377
Last modified:January 15, 2008 - v1
Checksum:iCFB0CAEAFFA7DD65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000730 Genomic DNA. Translation: ABX29783.1.
RefSeqiWP_000109906.1. NC_010079.1.

Genome annotation databases

EnsemblBacteriaiABX29783; ABX29783; USA300HOU_1778.
GeneIDi28380615.
KEGGisax:USA300HOU_1778.
PATRICi19598710. VBIStaAur36919_1876.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000730 Genomic DNA. Translation: ABX29783.1.
RefSeqiWP_000109906.1. NC_010079.1.

3D structure databases

ProteinModelPortaliA8Z4L3.
SMRiA8Z4L3.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX29783; ABX29783; USA300HOU_1778.
GeneIDi28380615.
KEGGisax:USA300HOU_1778.
PATRICi19598710. VBIStaAur36919_1876.

Phylogenomic databases

HOGENOMiHOG000271471.
KOiK01610.
OMAiRFIVKEP.

Enzyme and pathway databases

UniPathwayiUPA00138.

Family and domain databases

CDDicd00484. PEPCK_ATP. 1 hit.
Gene3Di3.40.449.10. 1 hit.
3.90.228.20. 2 hits.
HAMAPiMF_00453. PEPCK_ATP. 1 hit.
InterProiIPR001272. PEP_carboxykinase_ATP.
IPR013035. PEP_carboxykinase_C.
IPR008210. PEP_carboxykinase_N.
IPR015994. PEPCK_ATP_CS.
[Graphical view]
PfamiPF01293. PEPCK_ATP. 1 hit.
[Graphical view]
PIRSFiPIRSF006294. PEP_crbxkin. 1 hit.
SUPFAMiSSF68923. SSF68923. 1 hit.
TIGRFAMsiTIGR00224. pckA. 1 hit.
PROSITEiPS00532. PEPCK_ATP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPCKA_STAAT
AccessioniPrimary (citable) accession number: A8Z4L3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 15, 2008
Last modified: November 2, 2016
This is version 58 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.