Reviewed, UniProtKB/Swiss-Prot A8Z4K0 (ARSC_STAAT)

Names and origin

Protein names

Recommended name:
    Protein arsC
Alternative name(s):
    Arsenate reductase
    EC=1.20.4.-
    Arsenical pump modifier
    Low molecular weight protein-tyrosine-phosphatase
    EC=3.1.3.48

Gene names

Name:	arsC
Ordered Locus Names:	USA300HOU_1764

Organism

Staphylococcus aureus (strain USA300 / TCH1516) [Complete proteome] [HAMAP]

Taxonomic identifier

451516 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	131 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces arsenate [As(V)] to arsenite [As(III)] and dephosphorylates tyrosine phosphorylated proteins, low-MW aryl phosphates and natural and synthetic acyl phosphates. Could switch between different functions in different circumstances By similarity.
Catalytic activity	Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. HAMAP MF_01624 Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H2O. HAMAP MF_01624
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the low molecular weight phosphotyrosine protein phosphatase superfamily. ArsC family.

Ontologies

Keywords
Biological process	Arsenical resistance
Domain	Redox-active center
Molecular function	Hydrolase Oxidoreductase
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid dephosphorylation Inferred from electronic annotation. Source: InterPro response to arsenic Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arsenate reductase (thioredoxin) activity Inferred from electronic annotation. Source: HAMAP protein tyrosine phosphatase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 131	131	Protein arsC HAMAP MF_01624		PRO_1000088096
Sites
	Active site	10	1	Nucleophile; for reductase activity and phosphatase activity By similarity
	Active site	82	1	Nucleophile; for reductase activity By similarity
	Active site	89	1	Nucleophile; for reductase activity By similarity
Amino acid modifications
	Disulfide bond	10 ↔ 82		Redox-active; alternate By similarity
	Disulfide bond	82 ↔ 89		Redox-active; alternate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8Z4K0-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 9B6AA5A854D66C89	FASTA	131	14,687
	10 20 30 40 50 60 MTKKTIYFIC TGNSCRSQMA EGWAKQILAD DWNVYSAGIE THGVNPKAIE AMKEVGIDIS 70 80 90 100 110 120 NHTSDLIDNN IIKNSNLVVT LCSDADVNCP SLPTNVKKEH WGFDDPAGKP WSEFQRVRDE 130 IKIAIENFKS R

References

[1]

"Subtle genetic changes enhance virulence of methicillin resistant and sensitive Staphylococcus aureus." Highlander S.K., Hulten K.G., Qin X., Jiang H., Yerrapragada S., Mason E.O. Jr., Shang Y., Williams T.M., Fortunov R.M., Liu Y., Igboeli O., Petrosino J., Tirumalai M., Uzman A., Fox G.E., Cardenas A.M., Muzny D.M., Hemphill L. , Ding Y., Dugan S., Blyth P.R., Buhay C.J., Dinh H.H., Hawes A.C., Holder M., Kovar C.L., Lee S.L., Liu W., Nazareth L.V., Wang Q., Zhou J., Kaplan S.L., Weinstock G.M. BMC Microbiol. 7:99-99(2007) [PubMed: 17986343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000730 Genomic DNA. Translation: ABX29770.1.
RefSeq	YP_001575649.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5776201.
GenomeReviews	Gene locus USA300HOU_1764 in contig CP000730_GR.
KEGG	sax:USA300HOU_1764.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	EMRIVIT.
Family and domain databases
HAMAP	MF_01624. [Tree]
InterPro	IPR014064. Arsenate_reductase_StaphA. IPR017867. Tyr_phospatase_low_mol_wt. [Graphical view]
PANTHER	PTHR11717. Low_mwt_PTPase. 1 hit.
Pfam	PF01451. LMWPc. 1 hit. [Graphical view]
SMART	SM00226. LMWPc. 1 hit. [Graphical view]
TIGRFAMs	TIGR02691. arsC_pI258_fam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARSC_STAAT

Accession

Primary (citable) accession number: A8Z4K0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	January 15, 2008
Last modified:	November 3, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)