Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

A8Z4I6 (SYL_STAAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:USA300HOU_1749
OrganismStaphylococcus aureus (strain USA300 / TCH1516) [Complete proteome] [HAMAP]
Taxonomic identifier451516 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length805 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 805805Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000074847

Regions

Motif41 – 5212"HIGH" region HAMAP-Rule MF_00049
Motif577 – 5815"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5801ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8Z4I6 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: CCEF7840099E907F

FASTA80591,786
        10         20         30         40         50         60 
MLNYNHNQIE KKWQDYWDEN KTFKTNDNLG QKKFYALDMF PYPSGAGLHV GHPEGYTATD 

        70         80         90        100        110        120 
IISRYKRMQG YNVLHPMGWD AFGLPAEQYA LDTGNDPREF TKKNIQTFKR QIKELGFSYD 

       130        140        150        160        170        180 
WDREVNTTDP EYYKWTQWIF IQLYNKGLAY VDEVAVNWCP ALGTVLSNEE VIDGVSERGG 

       190        200        210        220        230        240 
HPVYRKPMKQ WVLKITEYAD QLLADLDDLD WPESLKDMQR NWIGRSEGAK VSFDVDNTEG 

       250        260        270        280        290        300 
KVEVFTTRPD TIYGASFLVL SPEHALVNSI TTDEYKEKVK AYQTEASKKS DLERTDLAKD 

       310        320        330        340        350        360 
KSGVFTGAYA TNPLSGEKVQ IWIADYVLST YGTGAIMAVP AHDDRDYEFA KKFDLPIIEV 

       370        380        390        400        410        420 
IEGGNVEEAA YTGEGKHINS GELDGLENEA AITKAIQLLE QKGAGEKKVN YKLRDWLFSR 

       430        440        450        460        470        480 
QRYWGEPIPV IHWEDGTMTT VPEEELPLLL PETDEIKPSG TGESPLANID SFVNVVDEKT 

       490        500        510        520        530        540 
GMKGRRETNT MPQWAGSCWY YLRYIDPKNE NMLADPEKLK HWLPVDLYIG GVEHAVLHLL 

       550        560        570        580        590        600 
YARFWHKVLY DLAIVPTKEP FQKLFNQGMI LGEGNEKMSK SKGNVINPDD IVQSHGADTL 

       610        620        630        640        650        660 
RLYEMFMGPL DAAIAWSEKG LDGSRRFLDR VWRLMVNEDG TLSSKIVTTN NKSLDKVYNQ 

       670        680        690        700        710        720 
TVKKVTEDFE TLGFNTAISQ LMVFINECYK VDEVYKPYIE GFVKMLAPIA PHIGEELWSK 

       730        740        750        760        770        780 
LGHEESITYQ PWPTYDEALL VDDEVEIVVQ VNGKLRAKIK IAKDTSKEEM QEIALSNDNV 

       790        800 
KASIEGKDIM KVIAVPQKLV NIVAK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000730 Genomic DNA. Translation: ABX29756.1.
RefSeqYP_001575635.1. NC_010079.1.

3D structure databases

ProteinModelPortalA8Z4I6.
SMRA8Z4I6. Positions 1-803.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING451516.USA300HOU_1749.

Proteomic databases

PRIDEA8Z4I6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX29756; ABX29756; USA300HOU_1749.
GeneID5776110.
KEGGsax:USA300HOU_1749.
PATRIC19598646. VBIStaAur36919_1844.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycSAUR451516:GJQ4-1770-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_STAAT
AccessionPrimary (citable) accession number: A8Z4I6
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 15, 2008
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries