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A8Z4H5 (TRMB_STAAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
Name:trmB
Ordered Locus Names:USA300HOU_1738
OrganismStaphylococcus aureus (strain USA300 / TCH1516) [Complete proteome] [HAMAP]
Taxonomic identifier451516 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliBacillalesStaphylococcus

Protein attributes

Sequence length214 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 214214tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057
PRO_1000136365

Regions

Region190 – 1934Substrate binding By similarity

Sites

Active site1171 By similarity
Binding site431S-adenosyl-L-methionine By similarity
Binding site681S-adenosyl-L-methionine By similarity
Binding site951S-adenosyl-L-methionine By similarity
Binding site1171S-adenosyl-L-methionine By similarity
Binding site1211Substrate By similarity
Binding site1531Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
A8Z4H5 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: 92987FE746A9DDD4

FASTA21425,276
        10         20         30         40         50         60 
MRVRYKPWAE DYLKDHPELV DMDGQHAGKM TEWFDKTQPI HIEIGSGMGQ FITTLAAQNP 

        70         80         90        100        110        120 
HINYISMERE KSIVYKVLDK VKEMGLTNLK IICNDAIELN EYFKDGEVSR IYLNFSDPWP 

       130        140        150        160        170        180 
KNRHAKRRLT YHTFLALYQQ ILNDEGDLHF KTDNRGLFAY SLESMSQFGM YFTKINLNLH 

       190        200        210 
QEDDGSNILT EYEKKFSDKG SRIYRMEAKF HSQK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000730 Genomic DNA. Translation: ABX29745.1.
RefSeqYP_001575624.1. NC_010079.1.

3D structure databases

ProteinModelPortalA8Z4H5.
SMRA8Z4H5. Positions 9-210.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING451516.USA300HOU_1738.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX29745; ABX29745; USA300HOU_1738.
GeneID5775448.
KEGGsax:USA300HOU_1738.
PATRIC19598626. VBIStaAur36919_1834.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000251689.
KOK03439.
OMAKTDDDEL.
OrthoDBEOG6K6VBC.
ProtClustDBPRK00121.

Enzyme and pathway databases

BioCycSAUR451516:GJQ4-1759-MONOMER.
UniPathwayUPA00989.

Family and domain databases

HAMAPMF_01057. tRNA_methyltr_TrmB.
InterProIPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_STAAT
AccessionPrimary (citable) accession number: A8Z4H5
Entry history
Integrated into UniProtKB/Swiss-Prot: April 14, 2009
Last sequence update: January 15, 2008
Last modified: April 16, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways