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Protein

Probable endonuclease 4

Gene

nfo

Organism
Staphylococcus aureus (strain USA300 / TCH1516)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 3 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi68Zinc 1UniRule annotation1
Metal bindingi109Zinc 1UniRule annotation1
Metal bindingi144Zinc 1UniRule annotation1
Metal bindingi144Zinc 2UniRule annotation1
Metal bindingi178Zinc 2UniRule annotation1
Metal bindingi181Zinc 3UniRule annotation1
Metal bindingi213Zinc 2UniRule annotation1
Metal bindingi226Zinc 3UniRule annotation1
Metal bindingi228Zinc 3UniRule annotation1
Metal bindingi258Zinc 2UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endonuclease 4UniRule annotation (EC:3.1.21.2UniRule annotation)
Alternative name(s):
Endodeoxyribonuclease IVUniRule annotation
Endonuclease IVUniRule annotation
Gene namesi
Name:nfoUniRule annotation
Ordered Locus Names:USA300HOU_1559
OrganismiStaphylococcus aureus (strain USA300 / TCH1516)
Taxonomic identifieri451516 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000768111 – 296Probable endonuclease 4Add BLAST296

Structurei

3D structure databases

ProteinModelPortaliA8Z497.
SMRiA8Z497.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000224895.
KOiK01151.
OMAiMKYVGAH.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo. 1 hit.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8Z497-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLLGSHVSMS GKKMLEGSAI EAHEYGETTF MIYTGAPQNT RRKSIEDLNI
60 70 80 90 100
TKGHEVMEKY GLSNIVVHAP YIINIANTTK PETFNLGVDF LQQEIERTQA
110 120 130 140 150
IGAKDIVLHP GAHVGAGVDA GINKIIEGLN EVLTNDNNVR IALETMAGKG
160 170 180 190 200
TEIGRSFEEL ARIIDGVHNN ERLSVCFDTC HTHDAGYNVK EDFDGVLNEF
210 220 230 240 250
DKIIGVDRIK VVHVNDSKND RGAQKDRHEN IGFGYIGFDA LNYIVHHDSF
260 270 280 290
KDIPKILETP YVGEDKKNKK PPYKLEIEML KQQQFDPELK NKVMQQ
Length:296
Mass (Da):33,159
Last modified:January 15, 2008 - v1
Checksum:i6B35AF57AF97914D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000730 Genomic DNA. Translation: ABX29566.1.
RefSeqiWP_000924214.1. NC_010079.1.

Genome annotation databases

EnsemblBacteriaiABX29566; ABX29566; USA300HOU_1559.
GeneIDi28380855.
KEGGisax:USA300HOU_1559.
PATRICi19598256. VBIStaAur36919_1648.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000730 Genomic DNA. Translation: ABX29566.1.
RefSeqiWP_000924214.1. NC_010079.1.

3D structure databases

ProteinModelPortaliA8Z497.
SMRiA8Z497.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX29566; ABX29566; USA300HOU_1559.
GeneIDi28380855.
KEGGisax:USA300HOU_1559.
PATRICi19598256. VBIStaAur36919_1648.

Phylogenomic databases

HOGENOMiHOG000224895.
KOiK01151.
OMAiMKYVGAH.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo. 1 hit.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEND4_STAAT
AccessioniPrimary (citable) accession number: A8Z497
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 15, 2008
Last modified: November 2, 2016
This is version 60 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.