A8Z3K5 (ALF1_STAAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Fructose-bisphosphate aldolase class 1
EC=4.1.2.13

Alternative name(s):
Fructose-bisphosphate aldolase class I
Short name=FBP aldolase

Gene names

Name:	fda
Ordered Locus Names:	USA300HOU_2599

Organism

Staphylococcus aureus (strain USA300 / TCH1516) [Complete proteome] [HAMAP]

Taxonomic identifier

451516 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Bacillales › Staphylococcaceae › Staphylococcus › Staphylococcus aureus › Staphylococcus aureus subsp. aureus › Staphylococcus aureus (strain USA300)

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate. HAMAP-Rule MF_00729
Pathway	Carbohydrate degradation; glycolysis; D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose: step 4/4. HAMAP-Rule MF_00729
Sequence similarities	Belongs to the class I fructose-bisphosphate aldolase family.

Ontologies

Keywords
Biological process	Glycolysis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycolysis Inferred from electronic annotation. Source: HAMAP
Molecular_function	fructose-bisphosphate aldolase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Fructose-bisphosphate aldolase class 1 HAMAP-Rule MF_00729		PRO_1000083326
Sites
	Active site	175	1	Proton acceptor By similarity
	Active site	212	1	Schiff-base intermediate with dihydroxyacetone-P By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8Z3K5 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 610A2E2FBDA7EB5F	FASTA	296	33,055
	10 20 30 40 50 60 MNKEQLEKMK NGKGFIAALD QSGGSTPKAL KEYGVNEDQY SNEDEMFQLV HDMRTRVVTS 70 80 90 100 110 120 PSFSPDKILG AILFEQTMDR EVESKYTADY LADKGVVPFL KVDKGLAEEQ NGVQLMKPID 130 140 150 160 170 180 NLDNLLDRAN ERHIFGTKMR SNILELNEQG IKDVVEQQFE VAKQIIAKGL VPIIEPEVNI 190 200 210 220 230 240 NAKDKAEIEK VLKAELKKGL DSLNADQLVM LKLTIPTEPN LYKELAEHPN VVRVVVLSGG 250 260 270 280 290 YSREKANELL KDNAELIASF SRALASDLRA DQSKEEFDKA LGDAVESIYD ASVNKN

References

[1]

"Subtle genetic changes enhance virulence of methicillin resistant and sensitive Staphylococcus aureus." Highlander S.K., Hulten K.G., Qin X., Jiang H., Yerrapragada S., Mason E.O. Jr., Shang Y., Williams T.M., Fortunov R.M., Liu Y., Igboeli O., Petrosino J., Tirumalai M., Uzman A., Fox G.E., Cardenas A.M., Muzny D.M., Hemphill L. , Ding Y., Dugan S., Blyth P.R., Buhay C.J., Dinh H.H., Hawes A.C., Holder M., Kovar C.L., Lee S.L., Liu W., Nazareth L.V., Wang Q., Zhou J., Kaplan S.L., Weinstock G.M. BMC Microbiol. 7:99-99(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USA300 / TCH1516.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000730 Genomic DNA. Translation: ABX30585.1.
RefSeq	YP_001576464.1. NC_010079.1.
3D structure databases
ProteinModelPortal	A8Z3K5.
SMR	A8Z3K5. Positions 1-293.
ModBase	Search...
Protein-protein interaction databases
STRING	451516.USA300HOU_2599.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABX30585; ABX30585; USA300HOU_2599.
GeneID	5775914.
KEGG	sax:USA300HOU_2599.
PATRIC	19600486. VBIStaAur36919_2711.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG3588.
HOGENOM	HOG000073502.
KO	K01623.
OMA	GTKMRSV.
ProtClustDB	PRK05377.
Enzyme and pathway databases
BioCyc	SAUR451516:GJQ4-2688-MONOMER.
UniPathway	UPA00109; UER00183.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_00729. FBP_aldolase_1.
InterPro	IPR000741. Aldolase_I. IPR013785. Aldolase_TIM. IPR023014. FBP_aldolase_I_bac. [Graphical view]
Pfam	PF00274. Glycolytic. 1 hit. [Graphical view]
PROSITE	PS00158. ALDOLASE_CLASS_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALF1_STAAT

Accession

Primary (citable) accession number: A8Z3K5

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	January 15, 2008
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program