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A8Z1R0

- ISDA_STAAT

UniProt

A8Z1R0 - ISDA_STAAT

Protein

Iron-regulated surface determinant protein A

Gene

isdA

Organism
Staphylococcus aureus (strain USA300 / TCH1516)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Transfers its hemin to hemin-free IsdC (apo-IsdC) directly probably through the activated holo-IsdA-apo-IsdC complex and driven by the higher affinity of apo-IsdC for the cofactor. The reaction is reversible. Binds transferrin, lactoferrin, heme, hemoglobin, hemin, fetuin, asialofetuin and protein A. Also binds fibronectin and chains B-beta and gamma of fibrinogen. Could play a role in the removal of heme from hemoglobin. The IsdA-mediated iron-acquisition system from transferrin could play only an ancillary role in the iron uptake whereas the siderophore-mediated iron-acquisition system from transferrin seems to play an essential or dominant role. May function as a reservoir for heme. Involved in adherence of S.aureus to human desquamated nasal epithelial cells and is required for nasal colonization. Protects S.aureus against the bactericidal protease activity of apolactoferrin in vitro and confers resistance to bovine lactoferricin. Also IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei75 – 751HemeBy similarity
    Binding sitei82 – 821HemeBy similarity
    Metal bindingi166 – 1661Iron (heme axial ligand)By similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW

    Keywords - Ligandi

    Heme, Iron, Metal-binding

    Enzyme and pathway databases

    BioCyciSAUR451516:GJQ4-1084-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Iron-regulated surface determinant protein A
    Alternative name(s):
    Fur-regulated protein A
    Staphylococcal transferrin-binding protein A
    Gene namesi
    Name:isdA
    Synonyms:frpA, stbA
    Ordered Locus Names:USA300HOU_1064
    OrganismiStaphylococcus aureus (strain USA300 / TCH1516)
    Taxonomic identifieri451516 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000000793: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    1. cell wall Source: UniProtKB-SubCell
    2. extracellular region Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell wall, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 4646By similarityAdd
    BLAST
    Chaini47 – 316270Iron-regulated surface determinant protein APRO_0000337760Add
    BLAST
    Propeptidei317 – 35034Removed by sortase APROSITE-ProRule annotationPRO_0000337761Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei316 – 3161Pentaglycyl murein peptidoglycan amidated threoninePROSITE-ProRule annotation

    Keywords - PTMi

    Peptidoglycan-anchor

    Expressioni

    Inductioni

    Repressed by fur in the presence of iron.By similarity

    Interactioni

    Subunit structurei

    Monomer. Interacts with IsdC By similarity.By similarity

    Protein-protein interaction databases

    STRINGi451516.USA300HOU_1064.

    Structurei

    3D structure databases

    ProteinModelPortaliA8Z1R0.
    SMRiA8Z1R0. Positions 63-184.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini62 – 184123NEATPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi313 – 3175LPXTG sorting signalPROSITE-ProRule annotation

    Domaini

    The NEAT domain is responsible for binding Fe3+ and Fe2+ heme and fibrinogen. The NEAT domain is an inhibitor of apolactoferrin activity, while the C-domain confers resistance to bovine lactoferricin By similarity.By similarity

    Sequence similaritiesi

    Contains 1 NEAT domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5386.
    HOGENOMiHOG000107381.
    KOiK14193.
    OMAiVNDDKKA.
    OrthoDBiEOG68SVXG.

    Family and domain databases

    InterProiIPR019948. Gram-positive_anchor.
    IPR019931. LPXTG_anchor.
    IPR006635. NEAT_dom.
    [Graphical view]
    PfamiPF00746. Gram_pos_anchor. 1 hit.
    PF05031. NEAT. 1 hit.
    [Graphical view]
    SMARTiSM00725. NEAT. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01167. LPXTG_anchor. 1 hit.
    PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
    PS50978. NEAT. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A8Z1R0-1 [UniParc]FASTAAdd to Basket

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    MTKHYLNSKY QSEQRSSAMK KITMGTASII LGSLVYIGAD SQQVNAATEA    50
    TNATNNQSTQ VSQATSQPIN FQVQKDGSSE KSHMDDYMQH PGKVIKQNNK 100
    YYFQTVLNNA SFWKEYKFYN ANNQELATTV VNDNKKADTR TINVAVEPGY 150
    KSLTTKVHIV VPQINYNHRY TTHLEFEKAI PTLADAAKPN NVKPVQPKPA 200
    QPKTPTEQTK PVQPKVEKVK PTVTTTSKVE DNHSTKVVST DTTKDQTKTQ 250
    TAHTVKTAQT AQEQNKVQTP VKDVATAKSE SNNQAVSDNK SQQTNKVTKH 300
    NETPKQASKA KELPKTGLTS VDNFISTVAF ATLALLGSLS LLLFKRKESK 350
    Length:350
    Mass (Da):38,746
    Last modified:January 15, 2008 - v1
    Checksum:i14882D25C0EA3CA6
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000730 Genomic DNA. Translation: ABX29083.1.
    RefSeqiYP_001574962.1. NC_010079.1.

    Genome annotation databases

    EnsemblBacteriaiABX29083; ABX29083; USA300HOU_1064.
    GeneIDi5776175.
    KEGGisax:USA300HOU_1064.
    PATRICi19597222. VBIStaAur36919_1131.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000730 Genomic DNA. Translation: ABX29083.1 .
    RefSeqi YP_001574962.1. NC_010079.1.

    3D structure databases

    ProteinModelPortali A8Z1R0.
    SMRi A8Z1R0. Positions 63-184.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 451516.USA300HOU_1064.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABX29083 ; ABX29083 ; USA300HOU_1064 .
    GeneIDi 5776175.
    KEGGi sax:USA300HOU_1064.
    PATRICi 19597222. VBIStaAur36919_1131.

    Phylogenomic databases

    eggNOGi COG5386.
    HOGENOMi HOG000107381.
    KOi K14193.
    OMAi VNDDKKA.
    OrthoDBi EOG68SVXG.

    Enzyme and pathway databases

    BioCyci SAUR451516:GJQ4-1084-MONOMER.

    Miscellaneous databases

    PROi A8Z1R0.

    Family and domain databases

    InterProi IPR019948. Gram-positive_anchor.
    IPR019931. LPXTG_anchor.
    IPR006635. NEAT_dom.
    [Graphical view ]
    Pfami PF00746. Gram_pos_anchor. 1 hit.
    PF05031. NEAT. 1 hit.
    [Graphical view ]
    SMARTi SM00725. NEAT. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01167. LPXTG_anchor. 1 hit.
    PROSITEi PS50847. GRAM_POS_ANCHORING. 1 hit.
    PS50978. NEAT. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: USA300 / TCH1516.

    Entry informationi

    Entry nameiISDA_STAAT
    AccessioniPrimary (citable) accession number: A8Z1R0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 20, 2008
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3