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A8Z1R0

- ISDA_STAAT

UniProt

A8Z1R0 - ISDA_STAAT

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Protein
Iron-regulated surface determinant protein A
Gene
isdA, frpA, stbA, USA300HOU_1064
Organism
Staphylococcus aureus (strain USA300 / TCH1516)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Transfers its hemin to hemin-free IsdC (apo-IsdC) directly probably through the activated holo-IsdA-apo-IsdC complex and driven by the higher affinity of apo-IsdC for the cofactor. The reaction is reversible. Binds transferrin, lactoferrin, heme, hemoglobin, hemin, fetuin, asialofetuin and protein A. Also binds fibronectin and chains B-beta and gamma of fibrinogen. Could play a role in the removal of heme from hemoglobin. The IsdA-mediated iron-acquisition system from transferrin could play only an ancillary role in the iron uptake whereas the siderophore-mediated iron-acquisition system from transferrin seems to play an essential or dominant role. May function as a reservoir for heme. Involved in adherence of S.aureus to human desquamated nasal epithelial cells and is required for nasal colonization. Protects S.aureus against the bactericidal protease activity of apolactoferrin in vitro and confers resistance to bovine lactoferricin. Also IsdA and/or IsdB promote resistance to hydrogen peroxide and killing by neutrophils By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei75 – 751Heme By similarity
Binding sitei82 – 821Heme By similarity
Metal bindingi166 – 1661Iron (heme axial ligand) By similarity

GO - Molecular functioni

  1. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

Keywords - Ligandi

Heme, Iron, Metal-binding

Enzyme and pathway databases

BioCyciSAUR451516:GJQ4-1084-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Iron-regulated surface determinant protein A
Alternative name(s):
Fur-regulated protein A
Staphylococcal transferrin-binding protein A
Gene namesi
Name:isdA
Synonyms:frpA, stbA
Ordered Locus Names:USA300HOU_1064
OrganismiStaphylococcus aureus (strain USA300 / TCH1516)
Taxonomic identifieri451516 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
ProteomesiUP000000793: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. cell wall Source: UniProtKB-SubCell
  2. extracellular region Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell wall, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 4646 By similarity
Add
BLAST
Chaini47 – 316270Iron-regulated surface determinant protein A
PRO_0000337760Add
BLAST
Propeptidei317 – 35034Removed by sortase A Reviewed prediction
PRO_0000337761Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei316 – 3161Pentaglycyl murein peptidoglycan amidated threonine Reviewed prediction

Keywords - PTMi

Peptidoglycan-anchor

Expressioni

Inductioni

Repressed by fur in the presence of iron By similarity.

Interactioni

Subunit structurei

Monomer. Interacts with IsdC By similarity.

Protein-protein interaction databases

STRINGi451516.USA300HOU_1064.

Structurei

3D structure databases

ProteinModelPortaliA8Z1R0.
SMRiA8Z1R0. Positions 63-184.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini62 – 184123NEAT
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi313 – 3175LPXTG sorting signal Reviewed prediction

Domaini

The NEAT domain is responsible for binding Fe3+ and Fe2+ heme and fibrinogen. The NEAT domain is an inhibitor of apolactoferrin activity, while the C-domain confers resistance to bovine lactoferricin By similarity.

Sequence similaritiesi

Contains 1 NEAT domain.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiCOG5386.
HOGENOMiHOG000107381.
KOiK14193.
OMAiVNDDKKA.
OrthoDBiEOG68SVXG.

Family and domain databases

InterProiIPR019948. Gram-positive_anchor.
IPR019931. LPXTG_anchor.
IPR006635. NEAT_dom.
[Graphical view]
PfamiPF00746. Gram_pos_anchor. 1 hit.
PF05031. NEAT. 1 hit.
[Graphical view]
SMARTiSM00725. NEAT. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01167. LPXTG_anchor. 1 hit.
PROSITEiPS50847. GRAM_POS_ANCHORING. 1 hit.
PS50978. NEAT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A8Z1R0-1 [UniParc]FASTAAdd to Basket

« Hide

MTKHYLNSKY QSEQRSSAMK KITMGTASII LGSLVYIGAD SQQVNAATEA    50
TNATNNQSTQ VSQATSQPIN FQVQKDGSSE KSHMDDYMQH PGKVIKQNNK 100
YYFQTVLNNA SFWKEYKFYN ANNQELATTV VNDNKKADTR TINVAVEPGY 150
KSLTTKVHIV VPQINYNHRY TTHLEFEKAI PTLADAAKPN NVKPVQPKPA 200
QPKTPTEQTK PVQPKVEKVK PTVTTTSKVE DNHSTKVVST DTTKDQTKTQ 250
TAHTVKTAQT AQEQNKVQTP VKDVATAKSE SNNQAVSDNK SQQTNKVTKH 300
NETPKQASKA KELPKTGLTS VDNFISTVAF ATLALLGSLS LLLFKRKESK 350
Length:350
Mass (Da):38,746
Last modified:January 15, 2008 - v1
Checksum:i14882D25C0EA3CA6
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000730 Genomic DNA. Translation: ABX29083.1.
RefSeqiYP_001574962.1. NC_010079.1.

Genome annotation databases

EnsemblBacteriaiABX29083; ABX29083; USA300HOU_1064.
GeneIDi5776175.
KEGGisax:USA300HOU_1064.
PATRICi19597222. VBIStaAur36919_1131.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000730 Genomic DNA. Translation: ABX29083.1 .
RefSeqi YP_001574962.1. NC_010079.1.

3D structure databases

ProteinModelPortali A8Z1R0.
SMRi A8Z1R0. Positions 63-184.
ModBasei Search...

Protein-protein interaction databases

STRINGi 451516.USA300HOU_1064.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX29083 ; ABX29083 ; USA300HOU_1064 .
GeneIDi 5776175.
KEGGi sax:USA300HOU_1064.
PATRICi 19597222. VBIStaAur36919_1131.

Phylogenomic databases

eggNOGi COG5386.
HOGENOMi HOG000107381.
KOi K14193.
OMAi VNDDKKA.
OrthoDBi EOG68SVXG.

Enzyme and pathway databases

BioCyci SAUR451516:GJQ4-1084-MONOMER.

Miscellaneous databases

PROi A8Z1R0.

Family and domain databases

InterProi IPR019948. Gram-positive_anchor.
IPR019931. LPXTG_anchor.
IPR006635. NEAT_dom.
[Graphical view ]
Pfami PF00746. Gram_pos_anchor. 1 hit.
PF05031. NEAT. 1 hit.
[Graphical view ]
SMARTi SM00725. NEAT. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01167. LPXTG_anchor. 1 hit.
PROSITEi PS50847. GRAM_POS_ANCHORING. 1 hit.
PS50978. NEAT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: USA300 / TCH1516.

Entry informationi

Entry nameiISDA_STAAT
AccessioniPrimary (citable) accession number: A8Z1R0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 15, 2008
Last modified: September 3, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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