A8Z1D4 (AROD_STAAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-dehydroquinate dehydratase
Short name=3-dehydroquinase
EC=4.2.1.10

Alternative name(s):
Type I DHQase

Gene names

Name:	aroD
Ordered Locus Names:	USA300HOU_0836

Organism

Staphylococcus aureus (strain USA300 / TCH1516) [Complete proteome] [HAMAP]

Taxonomic identifier

451516 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Staphylococcus

Protein attributes

Sequence length	238 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	3-dehydroquinate = 3-dehydroshikimate + H2O. HAMAP MF_00214
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 3/7. HAMAP MF_00214
Subunit structure	Homodimer By similarity. HAMAP MF_00214
Sequence similarities	Belongs to the type-I 3-dehydroquinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-dehydroquinate dehydratase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 238	238	3-dehydroquinate dehydratase HAMAP MF_00214		PRO_1000078050
Sites
	Active site	133	1	Proton acceptor By similarity
	Active site	160	1	Schiff-base intermediate with substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8Z1D4 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 108F24B67BDD76A6	FASTA	238	26,869
	10 20 30 40 50 60 MTHVEVVATI APQLSIEETL IQKINHRIDA IDVLELRIDQ IENVTVDQVA EMITKLKVMQ 70 80 90 100 110 120 DSFKLLVTYR TKLQGGYGQF TNDSYLNLIS DLANINGIDM IDIEWQADID IEKHQRIITH 130 140 150 160 170 180 LQQYNKEVVI SHHNFESTPP LDELQFIFFK MQKFNPEYVK LAVMPHNKND VLNLLQAMST 190 200 210 220 230 FSDTMDCKVV GISMSKLGLI SRTAQGVFGG ALTYGCIGVP QAPGQIDVTD LKAQVTLY

References

[1]

"Subtle genetic changes enhance virulence of methicillin resistant and sensitive Staphylococcus aureus." Highlander S.K., Hulten K.G., Qin X., Jiang H., Yerrapragada S., Mason E.O. Jr., Shang Y., Williams T.M., Fortunov R.M., Liu Y., Igboeli O., Petrosino J., Tirumalai M., Uzman A., Fox G.E., Cardenas A.M., Muzny D.M., Hemphill L. , Ding Y., Dugan S., Blyth P.R., Buhay C.J., Dinh H.H., Hawes A.C., Holder M., Kovar C.L., Lee S.L., Liu W., Nazareth L.V., Wang Q., Zhou J., Kaplan S.L., Weinstock G.M. BMC Microbiol. 7:99-99(2007) [PubMed: 17986343] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: USA300 / TCH1516.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000730 Genomic DNA. Translation: ABX28857.1.
RefSeq	YP_001574736.1. NC_010079.1.
3D structure databases
ProteinModelPortal	A8Z1D4.
SMR	A8Z1D4. Positions 3-238.
ModBase	Search...
Protein-protein interaction databases
STRING	A8Z1D4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTAT00000034497; EBSTAP00000033286; EBSTAG00000034495.
GeneID	5777057.
GenomeReviews	Gene locus USA300HOU_0836 in contig CP000730_GR.
KEGG	sax:USA300HOU_0836.
PATRIC	19596712. VBIStaAur36919_0876.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000025297.
HOGENOM	HBG732926.
OMA	IAKIAVM.
ProtClustDB	PRK13575.
Family and domain databases
HAMAP	MF_00214. AroD. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR001381. DHquinase_I. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K03785.
Pfam	PF01487. DHquinase_I. 1 hit. [Graphical view]
TIGRFAMs	TIGR01093. AroD. 1 hit.
PROSITE	PS01028. DEHYDROQUINASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROD_STAAT

Accession

Primary (citable) accession number: A8Z1D4

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program