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Protein

Sensor protein kinase WalK

Gene

walK

Organism
Staphylococcus aureus (strain USA300 / TCH1516)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Member of the two-component regulatory system WalK/WalR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to WalR.By similarity

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.By similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Two-component regulatory system

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciSAUR451516:GJQ4-21-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein kinase WalKCurated (EC:2.7.13.3By similarity)
Gene namesi
Name:walK
Ordered Locus Names:USA300HOU_0019
OrganismiStaphylococcus aureus (strain USA300 / TCH1516)
Taxonomic identifieri451516 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei14 – 3421HelicalSequence analysisAdd
BLAST
Transmembranei183 – 20321HelicalSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 608608Sensor protein kinase WalKPRO_0000353063Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei385 – 3851Phosphohistidine; by autocatalysisPROSITE-ProRule annotation

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliA8YYU2.
SMRiA8YYU2. Positions 223-599.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini204 – 25653HAMPPROSITE-ProRule annotationAdd
BLAST
Domaini261 – 33171PASPROSITE-ProRule annotationAdd
BLAST
Domaini314 – 37865PACPROSITE-ProRule annotationAdd
BLAST
Domaini382 – 600219Histidine kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 HAMP domain.PROSITE-ProRule annotation
Contains 1 histidine kinase domain.PROSITE-ProRule annotation
Contains 1 PAC (PAS-associated C-terminal) domain.PROSITE-ProRule annotation
Contains 1 PAS (PER-ARNT-SIM) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000223176.
KOiK07652.
OMAiSDGAIND.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8YYU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKWLKQLQSL HTKLVIVYVL LIIIGMQIIG LYFTNNLEKE LLDNFKKNIT
60 70 80 90 100
QYAKQLEISI EKVYDEKGSV NAQKDIQNLL SEYANRQEIG EIRFIDKDQI
110 120 130 140 150
IIATTKQSNR SLINQKANDS SVQKALSLGQ SNDHLILKDY GGGKDRVWVY
160 170 180 190 200
NIPVKVDKKV IGNIYIESKI NDVYNQLNNI NQIFIVGTAI SLLITVILGF
210 220 230 240 250
FIARTITKPI TDMRNQTVEM SRGNYTQRVK IYGNDEIGEL ALAFNNLSKR
260 270 280 290 300
VQEAQANTES EKRRLDSVIT HMSDGIIATD RRGRIRIVND MALKMLGMAK
310 320 330 340 350
EDIIGYYMLS VLSLEDEFKL EEIQENNDSF LLDLNEEEGL IARVNFSTIV
360 370 380 390 400
QETGFVTGYI AVLHDVTEQQ QVERERREFV ANVSHELRTP LTSMNSYIEA
410 420 430 440 450
LEEGAWKDEE LAPQFLSVTR EETERMIRLV NDLLQLSKMD NESDQINKEI
460 470 480 490 500
IDFNMFINKI INRHEMSAKD TTFIRDIPKK TIFTEFDPDK MTQVFDNVIT
510 520 530 540 550
NAMKYSRGDK RVEFHVKQNP LYNRMTIRIK DNGIGIPINK VDKIFDRFYR
560 570 580 590 600
VDKARTRKMG GTGLGLAISK EIVEAHNGRI WANSVEGQGT SIFITLPCEV

IEDGDWDE
Length:608
Mass (Da):69,924
Last modified:January 15, 2008 - v1
Checksum:iFBE26E64D2A37A8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000730 Genomic DNA. Translation: ABX28065.1.
RefSeqiWP_000871607.1. NC_010079.1.

Genome annotation databases

EnsemblBacteriaiABX28065; ABX28065; USA300HOU_0019.
KEGGisax:USA300HOU_0019.
PATRICi19595025. VBIStaAur36919_0052.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000730 Genomic DNA. Translation: ABX28065.1.
RefSeqiWP_000871607.1. NC_010079.1.

3D structure databases

ProteinModelPortaliA8YYU2.
SMRiA8YYU2. Positions 223-599.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX28065; ABX28065; USA300HOU_0019.
KEGGisax:USA300HOU_0019.
PATRICi19595025. VBIStaAur36919_0052.

Phylogenomic databases

HOGENOMiHOG000223176.
KOiK07652.
OMAiSDGAIND.

Enzyme and pathway databases

BioCyciSAUR451516:GJQ4-21-MONOMER.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
Gene3Di1.10.287.130. 1 hit.
3.30.565.10. 1 hit.
InterProiIPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
[Graphical view]
PfamiPF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF13426. PAS_9. 1 hit.
[Graphical view]
PRINTSiPR00344. BCTRLSENSOR.
SMARTiSM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
[Graphical view]
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiPS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiWALK_STAAT
AccessioniPrimary (citable) accession number: A8YYU2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 4, 2008
Last sequence update: January 15, 2008
Last modified: September 7, 2016
This is version 72 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.