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Protein

Sensor protein kinase WalK

Gene

walK

Organism
Staphylococcus aureus (strain USA300 / TCH1516)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Member of the two-component regulatory system WalK/WalR that regulates genes involved in autolysis, biofilm formation and cell wall metabolism. WalK functions as a sensor protein kinase which is autophosphorylated at a histidine residue and transfers its phosphate group to WalR.By similarity

Catalytic activityi

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine.By similarity

GO - Molecular functioni

Keywordsi

Molecular functionKinase, Transferase
Biological processTwo-component regulatory system
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sensor protein kinase WalKCurated (EC:2.7.13.3By similarity)
Gene namesi
Name:walK
Ordered Locus Names:USA300HOU_0019
OrganismiStaphylococcus aureus (strain USA300 / TCH1516)
Taxonomic identifieri451516 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcaceaeStaphylococcus

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei14 – 34HelicalSequence analysisAdd BLAST21
Transmembranei183 – 203HelicalSequence analysisAdd BLAST21

GO - Cellular componenti

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003530631 – 608Sensor protein kinase WalKAdd BLAST608

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei385Phosphohistidine; by autocatalysisPROSITE-ProRule annotation1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliA8YYU2.
SMRiA8YYU2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini204 – 256HAMPPROSITE-ProRule annotationAdd BLAST53
Domaini261 – 331PASPROSITE-ProRule annotationAdd BLAST71
Domaini314 – 378PACPROSITE-ProRule annotationAdd BLAST65
Domaini382 – 600Histidine kinasePROSITE-ProRule annotationAdd BLAST219

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000223176.
KOiK07652.
OMAiLTFWAVN.

Family and domain databases

CDDicd06225. HAMP. 1 hit.
cd00082. HisKA. 1 hit.
cd00130. PAS. 1 hit.
Gene3Di3.30.565.10. 1 hit.
InterProiView protein in InterPro
IPR003660. HAMP_dom.
IPR003594. HATPase_C.
IPR005467. His_kinase_dom.
IPR003661. HisK_dim/P.
IPR000014. PAS.
IPR000700. PAS-assoc_C.
IPR029151. Sensor-like.
IPR004358. Sig_transdc_His_kin-like_C.
PfamiView protein in Pfam
PF00672. HAMP. 1 hit.
PF02518. HATPase_c. 1 hit.
PF00512. HisKA. 1 hit.
PF13426. PAS_9. 1 hit.
PRINTSiPR00344. BCTRLSENSOR.
SMARTiView protein in SMART
SM00304. HAMP. 1 hit.
SM00387. HATPase_c. 1 hit.
SM00388. HisKA. 1 hit.
SM00091. PAS. 1 hit.
SUPFAMiSSF103190. SSF103190. 1 hit.
SSF47384. SSF47384. 1 hit.
SSF55785. SSF55785. 1 hit.
SSF55874. SSF55874. 1 hit.
TIGRFAMsiTIGR00229. sensory_box. 1 hit.
PROSITEiView protein in PROSITE
PS50885. HAMP. 1 hit.
PS50109. HIS_KIN. 1 hit.
PS50113. PAC. 1 hit.
PS50112. PAS. 1 hit.

Sequencei

Sequence statusi: Complete.

A8YYU2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKWLKQLQSL HTKLVIVYVL LIIIGMQIIG LYFTNNLEKE LLDNFKKNIT
60 70 80 90 100
QYAKQLEISI EKVYDEKGSV NAQKDIQNLL SEYANRQEIG EIRFIDKDQI
110 120 130 140 150
IIATTKQSNR SLINQKANDS SVQKALSLGQ SNDHLILKDY GGGKDRVWVY
160 170 180 190 200
NIPVKVDKKV IGNIYIESKI NDVYNQLNNI NQIFIVGTAI SLLITVILGF
210 220 230 240 250
FIARTITKPI TDMRNQTVEM SRGNYTQRVK IYGNDEIGEL ALAFNNLSKR
260 270 280 290 300
VQEAQANTES EKRRLDSVIT HMSDGIIATD RRGRIRIVND MALKMLGMAK
310 320 330 340 350
EDIIGYYMLS VLSLEDEFKL EEIQENNDSF LLDLNEEEGL IARVNFSTIV
360 370 380 390 400
QETGFVTGYI AVLHDVTEQQ QVERERREFV ANVSHELRTP LTSMNSYIEA
410 420 430 440 450
LEEGAWKDEE LAPQFLSVTR EETERMIRLV NDLLQLSKMD NESDQINKEI
460 470 480 490 500
IDFNMFINKI INRHEMSAKD TTFIRDIPKK TIFTEFDPDK MTQVFDNVIT
510 520 530 540 550
NAMKYSRGDK RVEFHVKQNP LYNRMTIRIK DNGIGIPINK VDKIFDRFYR
560 570 580 590 600
VDKARTRKMG GTGLGLAISK EIVEAHNGRI WANSVEGQGT SIFITLPCEV

IEDGDWDE
Length:608
Mass (Da):69,924
Last modified:January 15, 2008 - v1
Checksum:iFBE26E64D2A37A8D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000730 Genomic DNA. Translation: ABX28065.1.
RefSeqiWP_000871607.1. NC_010079.1.

Genome annotation databases

EnsemblBacteriaiABX28065; ABX28065; USA300HOU_0019.
GeneIDi31212846.
KEGGisax:USA300HOU_0019.

Similar proteinsi

Entry informationi

Entry nameiWALK_STAAT
AccessioniPrimary (citable) accession number: A8YYU2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 4, 2008
Last sequence update: January 15, 2008
Last modified: September 27, 2017
This is version 78 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program