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A8YYH5

- BIOB_STAAT

UniProt

A8YYH5 - BIOB_STAAT

Protein

Biotin synthase

Gene

bioB

Organism
Staphylococcus aureus (strain USA300 / TCH1516)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 48 (01 Oct 2014)
      Sequence version 1 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi61 – 611Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi65 – 651Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi68 – 681Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi104 – 1041Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi137 – 1371Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi197 – 1971Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi267 – 2671Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciSAUR451516:GJQ4-2498-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:USA300HOU_2409
    OrganismiStaphylococcus aureus (strain USA300 / TCH1516)
    Taxonomic identifieri451516 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesStaphylococcus
    ProteomesiUP000000793: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 335335Biotin synthasePRO_0000381654Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi451516.USA300HOU_2409.

    Structurei

    3D structure databases

    ProteinModelPortaliA8YYH5.
    SMRiA8YYH5. Positions 30-315.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiRIMMPAS.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    A8YYH5-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNLAKRILQG EQLTKETVLK IYEDTNIDTL DLLNEAYILR KHYFGKKVKL    50
    NMILNAKSGI CPENCGYCGQ SRDIKQKQRY ALIPEEQIID GAKVAHDNHI 100
    GTYCIVMSGR GPSDKEVDHI SNTVRTIKSQ HPQLKICACL GLTNDEQAKK 150
    LKSAGVDRYN HNINTSENYH DNVVTTHSYK DRTDTIELMK ANNISPCSGV 200
    ICGMGESNQD IVDMAFALKE MDADSIPINF LHPIKGTKFG SMDDLTPMKC 250
    LRIVALFRLI NPTKEIRIAG GREVNLRSLQ PLALKAANSI FVGDYLITGG 300
    QPNQLDYDMI NDLGFEIDYD TCENKENKNE VSRAN 335
    Length:335
    Mass (Da):37,559
    Last modified:January 15, 2008 - v1
    Checksum:i0C7DAB4ECCB68A5B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000730 Genomic DNA. Translation: ABX30399.1.
    RefSeqiYP_001576278.1. NC_010079.1.

    Genome annotation databases

    EnsemblBacteriaiABX30399; ABX30399; USA300HOU_2409.
    GeneIDi5775333.
    KEGGisax:USA300HOU_2409.
    PATRICi19600102. VBIStaAur36919_2519.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000730 Genomic DNA. Translation: ABX30399.1 .
    RefSeqi YP_001576278.1. NC_010079.1.

    3D structure databases

    ProteinModelPortali A8YYH5.
    SMRi A8YYH5. Positions 30-315.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 451516.USA300HOU_2409.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABX30399 ; ABX30399 ; USA300HOU_2409 .
    GeneIDi 5775333.
    KEGGi sax:USA300HOU_2409.
    PATRICi 19600102. VBIStaAur36919_2519.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi RIMMPAS.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci SAUR451516:GJQ4-2498-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: USA300 / TCH1516.

    Entry informationi

    Entry nameiBIOB_STAAT
    AccessioniPrimary (citable) accession number: A8YYH5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 48 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3