A8YXS0 (FABH_LACH4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase 3
EC=2.3.1.41

Alternative name(s):
3-oxoacyl-[acyl-carrier-protein] synthase III
Beta-ketoacyl-ACP synthase III
Short name=KAS III

Gene names

Name:	fabH
Ordered Locus Names:	lhv_1932

Organism

Lactobacillus helveticus (strain DPC 4571) [Complete proteome] [HAMAP]

Taxonomic identifier

405566 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	327 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Catalyzes the first condensation reaction which initiates fatty acid synthesis and may therefore play a role in governing the total rate of fatty acid production. Possesses both acetoacetyl-ACP synthase and acetyl transacylase activities. Its substrate specificity determines the biosynthesis of branched-chain and/or straight-chain of fatty acids By similarity. HAMAP MF_01815
Catalytic activity	Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein]. HAMAP MF_01815
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP MF_01815
Subunit structure	Homodimer By similarity. HAMAP MF_01815
Subcellular location	Cytoplasm Probable HAMAP MF_01815.
Domain	The last Arg residue of the ACP-binding site is essential for the weak association between ACP/AcpP and FabH By similarity. HAMAP MF_01815
Sequence similarities	Belongs to the FabH family.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Lipid synthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	fatty acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxoacyl-[acyl-carrier-protein] synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 327	327	3-oxoacyl-[acyl-carrier-protein] synthase 3 HAMAP MF_01815		PRO_1000073681
Regions
	Region	255 – 259	5	ACP-binding By similarity
Sites
	Active site	114	1	By similarity
	Active site	254	1	By similarity
	Active site	284	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8YXS0 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 94722533A6DBD1D7	FASTA	327	35,344
	10 20 30 40 50 60 MKFEDFKIMA TASSAPDHVV TNDELATMMD TSDEWITQRT GIKRRRIATE ETTSSMCTDV 70 80 90 100 110 120 ATQLIAQSDL TAKDIDLIAV ATMSPDYLTP SVSAMVQGNI GADHAIAFDI DAACSGFVYG 130 140 150 160 170 180 LHLVKQMLIA NQQKNAILIG GETLSKLLDW SDRSTAVLFG DGAGGVLIRN TAVDKGSFIS 190 200 210 220 230 240 EDLRTLGNLG QYLTAGQTGN PSPFATDQQP FSPFFKMSGR RVYSFAVKNV PESINDALKQ 250 260 270 280 290 300 ANLTADEVDC FVLHQANRRI VERIADELAV SMAKFPINID EYGNTAAASE PILLDQLVKQ 310 320 KIIKRGDVIA LSGFGGGLTV GTMIMKY

References

[1]

"Genome sequence of Lactobacillus helveticus: an organism distinguished by selective gene loss and IS element expansion." Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K., McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F., Beresford T., Ross R.P. J. Bacteriol. 190:727-735(2008) [PubMed: 17993529] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DPC 4571.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000517 Genomic DNA. Translation: ABX27770.1.
RefSeq	YP_001578074.1. NC_010080.1.
3D structure databases
ProteinModelPortal	A8YXS0.
ModBase	Search...
Protein-protein interaction databases
STRING	A8YXS0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5771555.
GenomeReviews	Gene locus lhv_1932 in contig CP000517_GR.
KEGG	lhe:lhv_1932.
PATRIC	22236212. VBILacHel91643_2047.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG649927.
OMA	NDAWIVE.
ProtClustDB	PRK09352.
Enzyme and pathway databases
BioCyc	LHEL405566:LHV_1932-MONOMER.
Family and domain databases
HAMAP	MF_01815. FabH. [Tree]
InterPro	IPR013751. ACP_syn_III. IPR013747. ACP_syn_III_C. IPR004655. FabH_synth. IPR016039. Thiolase-like. IPR016038. Thiolase-like_subgr. [Graphical view]
Gene3D	G3DSA:3.40.47.10. Thiolase-like_subgr. 2 hits.
KO	K00648.
Pfam	PF08545. ACP_syn_III. 1 hit. PF08541. ACP_syn_III_C. 1 hit. [Graphical view]
SUPFAM	SSF53901. Thiolase-like. 1 hit.
TIGRFAMs	TIGR00747. FabH. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABH_LACH4

Accession

Primary (citable) accession number: A8YXS0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program