A8YXI3 (ALR_LACH4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	lhv_0285

Organism

Lactobacillus helveticus (strain DPC 4571) [Complete proteome] [HAMAP]

Taxonomic identifier

405566 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	376 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Provides the D-alanine required for cell wall biosynthesis By similarity. HAMAP MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP MF_01201 Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	alanine metabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 376	376	Alanine racemase HAMAP MF_01201		PRO_1000073098
Sites
	Active site	40	1	Proton acceptor; specific for D-alanine By similarity
	Active site	270	1	Proton acceptor; specific for L-alanine By similarity
Amino acid modifications
	Modified residue	40	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8YXI3 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: AD4E0A2C88616D48	FASTA	376	41,636
	10 20 30 40 50 60 MVPGYYRPAV VKVNLGAIRR NIKNEMQHLE PNQKMLAVVK ANGYGHGAVE VAKVAEEEGA 70 80 90 100 110 120 AGFCVAILDE ALELRRADIV KPILVLGVVS PEYAPIAAVN NVSLTVPNLE WLKDAEKYLA 130 140 150 160 170 180 KENLQLKIHL GIDSGMGRIG FNEDDEFVAA NKFLENNNNF FIEGMFAHFA SADSDDETYF 190 200 210 220 230 240 KHQCEKFKHM KSLLTVKPKW IHVDNTAASI FHSGIKSDLV RFGIGIYGLN PSSNPSSPDL 250 260 270 280 290 300 TSAIELEPAL SFESELTHVK TIHKGDGVGY GSTFMAEEDT IIGTVPVGYA DGWIRKYQGF 310 320 330 340 350 360 KVKVGDEYCS IVGRICMDQF MVKMPKKMLV GTKVVIISNN PSDPNNIKAA ADYVDTIHYE 370 VACLLNDRLP RVYYEK

References

[1]

"Genome sequence of Lactobacillus helveticus: an organism distinguished by selective gene loss and IS element expansion." Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K., McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F., Beresford T., Ross R.P. J. Bacteriol. 190:727-735(2008) [PubMed: 17993529] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DPC 4571.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000517 Genomic DNA. Translation: ABX26514.1.
RefSeq	YP_001576805.1. NC_010080.1.
3D structure databases
ProteinModelPortal	A8YXI3.
ModBase	Search...
Protein-protein interaction databases
STRING	A8YXI3.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5772528.
GenomeReviews	Gene locus lhv_0285 in contig CP000517_GR.
KEGG	lhe:lhv_0285.
PATRIC	22232578. VBILacHel91643_0295.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG712172.
OMA	TRKEDAN.
ProtClustDB	CLSK925011.
Enzyme and pathway databases
BioCyc	LHEL405566:LHV_0285-MONOMER.
Family and domain databases
HAMAP	MF_01201. Ala_racemase. [Tree]
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. IPR020622. Ala_racemase_pyridoxalP-BS. [Graphical view]
Gene3D	G3DSA:2.40.37.10. Ala_racemase/Decarboxylase_C. 1 hit.
KO	K01775.
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. Alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_LACH4

Accession

Primary (citable) accession number: A8YXI3

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program