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A8YWU4 (SYL_LACH4) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:lhv_1726
OrganismLactobacillus helveticus (strain DPC 4571) [Complete proteome] [HAMAP]
Taxonomic identifier405566 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length804 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 804804Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000091328

Regions

Motif39 – 5012"HIGH" region HAMAP-Rule MF_00049
Motif573 – 5775"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5761ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8YWU4 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: 430DC21235A47AEB

FASTA80492,396
        10         20         30         40         50         60 
MYNHKVVEKK WQDYWAKHDT FKTGTDPKKK NYYALDMFPF PSGKGLHVGH PEGYTATDIV 

        70         80         90        100        110        120 
SRMKRAQGYN VLHPMGWDAF GLPTEQYALK TGEDPAVVTK NNIANFKRQL NKLGFSYDWD 

       130        140        150        160        170        180 
REITTSDPNY YKWTQWVFEQ MYKKGLAYEA EVPVNWSPDL GTVVANEEVI DGKTERGGYP 

       190        200        210        220        230        240 
VYRRNMRQWM LKMTAYADRL LEDLDDLDWP EPVKEMQRNW IGRSVGAQVT FKIKDSDKTF 

       250        260        270        280        290        300 
DIFTTRPDTL FGCSYTVLAP ENKLVQEITT DGHRDEVNAY IKKIESKSDL ERTDLNKDKT 

       310        320        330        340        350        360 
GVFTGAYAIN PVNGKEVPIW ISDYVLASYG TGAVMAVPAH DDRDYAFAKK FDLPINQVLE 

       370        380        390        400        410        420 
GGDLSKAAFT EDGPHINSEF LNGLNIKDAK KKMVDWLEEH NCGEKKVNYK LRDWDFSRQR 

       430        440        450        460        470        480 
YWGEPIPVIH WEDGTTSLVP EDQLPLRLPH ATDIKPSGTP ESPLANLTDW VNVVDEMGRK 

       490        500        510        520        530        540 
GKRETNTMPN WAGSSWYYLR YVDPHNDKEL ADYDLLKQWL PVDLYIGGAE HAVRHLLYAR 

       550        560        570        580        590        600 
FWHKVLYDLG VVPTKEPFQR LYNQGLILKN HEKMSKSKGN VVNPDEVIDE YGADSLRMYE 

       610        620        630        640        650        660 
MFMGPLDASI DWDDNGPAST KKFLDRVWRL FVNDLDLKAI PQERIVDKND GELDKVYAET 

       670        680        690        700        710        720 
VKKVTEDFDA LHFNTAISQM MVFINAAQKA KTIPREYVEG FVKLLAPVAP HMMEEIWQVF 

       730        740        750        760        770        780 
GHDESITYAK WPTYDPAKLV ESTVEIMVQV NGKLRGKFEA AKDADRDDVQ KQAMALPHVQ 

       790        800 
KFLEGKDVKK VIVVPNKIVN IVAK 

« Hide

References

[1]"Genome sequence of Lactobacillus helveticus: an organism distinguished by selective gene loss and IS element expansion."
Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K., McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F., Beresford T., Ross R.P.
J. Bacteriol. 190:727-735(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DPC 4571.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000517 Genomic DNA. Translation: ABX27605.1.
RefSeqYP_001577906.1. NC_010080.1.

3D structure databases

ProteinModelPortalA8YWU4.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING405566.lhv_1726.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX27605; ABX27605; lhv_1726.
GeneID5771243.
KEGGlhe:lhv_1726.
PATRIC22235748. VBILacHel91643_1822.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200748.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycLHEL405566:GJEN-1513-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_LACH4
AccessionPrimary (citable) accession number: A8YWU4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 24, 2009
Last sequence update: January 15, 2008
Last modified: April 16, 2014
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries