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A8YWH0

- TRMB_LACH4

UniProt

A8YWH0 - TRMB_LACH4

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Protein
tRNA (guanine-N(7)-)-methyltransferase
Gene
trmB, lhv_1653
Organism
Lactobacillus helveticus (strain DPC 4571)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei45 – 451S-adenosyl-L-methionine By similarity
Binding sitei70 – 701S-adenosyl-L-methionine By similarity
Binding sitei97 – 971S-adenosyl-L-methionine By similarity
Active sitei119 – 1191 By similarity
Binding sitei119 – 1191S-adenosyl-L-methionine By similarity
Binding sitei123 – 1231Substrate By similarity
Binding sitei155 – 1551Substrate By similarity

GO - Molecular functioni

  1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciLHEL405566:GJEN-1455-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferase (EC:2.1.1.33)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene namesi
Name:trmB
Ordered Locus Names:lhv_1653
OrganismiLactobacillus helveticus (strain DPC 4571)
Taxonomic identifieri405566 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000790: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 217217tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation
PRO_1000072997Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi405566.lhv_1653.

Structurei

3D structure databases

ProteinModelPortaliA8YWH0.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni125 – 1306Interaction with RNA Reviewed prediction
Regioni195 – 1984Substrate binding By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000251689.
KOiK03439.
OMAiDNVMTEY.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8YWH0-1 [UniParc]FASTAAdd to Basket

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MRLRNKPWAV KLVNEHPESV LQNPDPEKKI DWAARFGNDN TIEIEVGSGK    50
GHFITTLAEN NPDKNYVALE LQTTAAGIIL RTKLEKGLDN LQILRGDAAD 100
INCFFPENST NVIYLNFSDP WPKTRHEKRR LTYKSFLAKY QQVLTKDGHI 150
EFKTDNSGLF AYSVQSMNNF GMHFDFVSVD LHHEKPEIVE KNIETEYEHK 200
FAAKGNPIYA LHADFEA 217
Length:217
Mass (Da):24,894
Last modified:January 15, 2008 - v1
Checksum:i7E9CA979625A0A91
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000517 Genomic DNA. Translation: ABX27556.1.
RefSeqiYP_001577857.1. NC_010080.1.

Genome annotation databases

EnsemblBacteriaiABX27556; ABX27556; lhv_1653.
GeneIDi5771306.
KEGGilhe:lhv_1653.
PATRICi22235582. VBILacHel91643_1740.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000517 Genomic DNA. Translation: ABX27556.1 .
RefSeqi YP_001577857.1. NC_010080.1.

3D structure databases

ProteinModelPortali A8YWH0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 405566.lhv_1653.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX27556 ; ABX27556 ; lhv_1653 .
GeneIDi 5771306.
KEGGi lhe:lhv_1653.
PATRICi 22235582. VBILacHel91643_1740.

Phylogenomic databases

eggNOGi COG0220.
HOGENOMi HOG000251689.
KOi K03439.
OMAi DNVMTEY.
OrthoDBi EOG6K6VBC.

Enzyme and pathway databases

UniPathwayi UPA00989 .
BioCyci LHEL405566:GJEN-1455-MONOMER.

Family and domain databases

Gene3Di 3.40.50.150. 1 hit.
HAMAPi MF_01057. tRNA_methyltr_TrmB.
InterProi IPR029063. SAM-dependent_MTases-like.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view ]
Pfami PF02390. Methyltransf_4. 1 hit.
[Graphical view ]
SUPFAMi SSF53335. SSF53335. 1 hit.
TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Lactobacillus helveticus: an organism distinguished by selective gene loss and IS element expansion."
    Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K., McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F., Beresford T., Ross R.P.
    J. Bacteriol. 190:727-735(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DPC 4571.

Entry informationi

Entry nameiTRMB_LACH4
AccessioniPrimary (citable) accession number: A8YWH0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 15, 2008
Last modified: June 11, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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