A8YW85 (PURL_LACH4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	lhv_1529

Organism

Lactobacillus helveticus (strain DPC 4571) [Complete proteome] [HAMAP]

Taxonomic identifier

405566 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Bacilli › Lactobacillales › Lactobacillaceae › Lactobacillus › Lactobacillus helveticus

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP-Rule MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP-Rule MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 743	743	Phosphoribosylformylglycinamidine synthase 2 HAMAP-Rule MF_00420		PRO_1000072298
Regions
	Nucleotide binding	110 – 121	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A8YW85 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 18AFA1E955ACD49B	FASTA	743	81,845
	10 20 30 40 50 60 MKQAMTPEEI KEKKPYLDWS LTEHEYDYIC DKLLKRLPNY TEIGLFSAMW SEHCSYKKSK 70 80 90 100 110 120 PVLRLFPTKG KRVVQGPGEG AGVVDIDDGQ AVVFKAESHN HPTTVEPYQG AATGVGGILR 130 140 150 160 170 180 DVFSMGARPV AILDSLHFGE LKNNPTMRYK MEETIRGVGD YGNCIGIPNL GGETTFDSCY 190 200 210 220 230 240 NGNILLNAMC VGLMNIKDME HGKAVGLGNS IMYVGAKTGR DGIHGATFAS ADFSKEHETQ 250 260 270 280 290 300 RSAVQVGDPF MEKLLMEACL ELILKHRDWL VGIQDMGAAG IVSSSAEMAT EGNAGMELNL 310 320 330 340 350 360 DLVPQREPNM SAYEIMLSES QERMLLCVKK GHEEDVKKIF KDYNLDAVTI GRVIEGENYV 370 380 390 400 410 420 LYHEGEKVCD IPVRSLTEDV LEEPSKEIKP QYIIDAEKMP AWRPSFESSG EILKRLLNQP 430 440 450 460 470 480 TIANDQFITQ QYDSQVRSNT IVKPGSDAGV LRIRHTKKAI AMCTDTNGRF VYLNPKIGGQ 490 500 510 520 530 540 RSVIESVCNI VASGAKPLAI TDCLNYGDPN DPEIFWSLRE SCQGIADACR IFETPVVSGN 550 560 570 580 590 600 VSLYNENDGQ AIYPSPMIGM VGLIKNTDYV IPMHVQEADD IVYLVGKTTA DFAGSEVQKM 610 620 630 640 650 660 LQGKISGLPE APNLELIHDY LKNLHKAMTQ GLVTSAHDLS EGGLAVSLAE SVFDTEFGLN 670 680 690 700 710 720 INLSEFDKTL LFSETPGRLI VSVKPENKSA FESLLGSAVQ EIGRVTDQRK LSIKLANDEL 730 740 NTDVASLEKI WKESIPCLMK SKA

References

[1]

"Genome sequence of Lactobacillus helveticus: an organism distinguished by selective gene loss and IS element expansion." Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K., McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F., Beresford T., Ross R.P. J. Bacteriol. 190:727-735(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DPC 4571.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000517 Genomic DNA. Translation: ABX27471.1.
RefSeq	YP_001577772.1. NC_010080.1.
3D structure databases
ProteinModelPortal	A8YW85.
ModBase	Search...
Protein-protein interaction databases
STRING	405566.lhv_1529.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ABX27471; ABX27471; lhv_1529.
GeneID	5771397.
KEGG	lhe:lhv_1529.
PATRIC	22235325. VBILacHel91643_1612.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0046.
HOGENOM	HOG000238227.
KO	K01952.
OMA	WSEHCCY.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	LHEL405566:GJEN-1362-MONOMER.
UniPathway	UPA00074; UER00128.
Family and domain databases
HAMAP	MF_00420. PurL_2.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_LACH4

Accession

Primary (citable) accession number: A8YW85

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	January 15, 2008
Last modified:	May 1, 2013
This is version 37 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program