A8YW78 (SERC_LACH4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoserine aminotransferase
EC=2.6.1.52

Alternative name(s):
Phosphohydroxythreonine aminotransferase
Short name=PSAT

Gene names

Name:	serC
Ordered Locus Names:	lhv_0048

Organism

Lactobacillus helveticus (strain DPC 4571) [Complete proteome] [HAMAP]

Taxonomic identifier

405566 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	373 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine By similarity. HAMAP MF_00160
Catalytic activity	O-phospho-L-serine + 2-oxoglutarate = 3-phosphonooxypyruvate + L-glutamate. HAMAP MF_00160 4-phosphonooxy-L-threonine + 2-oxoglutarate = (3R)-3-hydroxy-2-oxo-4-phosphonooxybutanoate + L-glutamate. HAMAP MF_00160
Cofactor	Binds 1 pyridoxal phosphate per subunit By similarity. HAMAP MF_00160
Pathway	Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 2/3. HAMAP MF_00160
Subunit structure	Homodimer By similarity. HAMAP MF_00160
Subcellular location	Cytoplasm By similarity HAMAP MF_00160.
Sequence similarities	Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. SerC subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Serine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Aminotransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	L-serine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	O-phospho-L-serine:2-oxoglutarate aminotransferase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 373	373	Phosphoserine aminotransferase HAMAP MF_00160		PRO_1000071543
Regions
	Region	75 – 76	2	Pyridoxal phosphate binding By similarity
	Region	236 – 237	2	Pyridoxal phosphate binding By similarity
Sites
	Binding site	41	1	L-glutamate By similarity
	Binding site	101	1	Pyridoxal phosphate By similarity
	Binding site	152	1	Pyridoxal phosphate By similarity
	Binding site	172	1	Pyridoxal phosphate By similarity
	Binding site	195	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	196	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8YW78 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 9090F84548F80786	FASTA	373	41,162
	10 20 30 40 50 60 MTVYNFAAGP ATLPDPVIKQ IQEELPSLQG SGMSILEISH RSQMFDKIID TAKQDIKDLM 70 80 90 100 110 120 HVPDNYHILF FQGGGTGQFA AVPMNLATKH KRIALLDSGH WATRAGDEAA NLGVTVDVLD 130 140 150 160 170 180 STKDKHYQEL PHMPHAISAS DYDYLHITTN NTIEGTAYHT LPEHGDVTLV GDLSSNFMAE 190 200 210 220 230 240 EYQVSDFGLI FGGVQKNLGP AGVTVVIVRD DLVNHVDHIP SILNYELFVK KNSMFNTPPV 250 260 270 280 290 300 FAIYATGLVL KWLKQQGGIA GIEALNKKKS ALLYDFLDQS TLFHNDIKKT DRSLTNIPFK 310 320 330 340 350 360 TTDPVLDKQV IAEADQAGLK NLKGHRSVGG LRASLYNAMP LAGVQALVDF LYNFEKQHKN 370 NTMGKRYVSS KNI

References

[1]

"Genome sequence of Lactobacillus helveticus: an organism distinguished by selective gene loss and IS element expansion." Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K., McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F., Beresford T., Ross R.P. J. Bacteriol. 190:727-735(2008) [PubMed: 17993529] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DPC 4571.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000517 Genomic DNA. Translation: ABX26333.1.
RefSeq	YP_001576620.1. NC_010080.1.
3D structure databases
ProteinModelPortal	A8YW78.
ModBase	Search...
Protein-protein interaction databases
STRING	A8YW78.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	5772750.
GenomeReviews	Gene locus lhv_0048 in contig CP000517_GR.
KEGG	lhe:lhv_0048.
PATRIC	22232068. VBILacHel91643_0051.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG289982.
OMA	YEVLFLQ.
ProtClustDB	PRK05355.
Enzyme and pathway databases
BioCyc	LHEL405566:LHV_0048-MONOMER.
Family and domain databases
HAMAP	MF_00160. SerC_aminotrans_5. [Tree]
InterPro	IPR000192. Aminotrans_V/Cys_dSase. IPR022278. Pser_aminoTfrase. IPR003248. Pser_aminoTfrase_subgr. IPR015424. PyrdxlP-dep_Trfase_major_dom. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
KO	K00831.
PANTHER	PTHR21152:SF1. PTHR21152:SF1. 1 hit.
Pfam	PF00266. Aminotran_5. 1 hit. [Graphical view]
PIRSF	PIRSF000525. SerC. 1 hit.
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 1 hit.
TIGRFAMs	TIGR01364. SerC_1. 1 hit.
PROSITE	PS00595. AA_TRANSFER_CLASS_5. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SERC_LACH4

Accession

Primary (citable) accession number: A8YW78

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	January 15, 2008
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program