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Reviewed, UniProtKB/Swiss-Prot A8YVZ5 (PYRC_LACH4)

Last modified September 2, 2008. Version 7. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Dihydroorotase
      Short name=DHOase
    EC=3.5.2.3
Gene names
Name: pyrC
Ordered Locus Names: lhv_1445
OrganismLactobacillus helveticus (strain DPC 4571) [Complete proteome] [HAMAP]
Taxonomic identifier405566 [NCBI]
Taxonomic lineageBacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

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Protein attributes

Sequence length425 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceInferred from homology.

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General annotation (Comments)

Catalytic activity

(S)-dihydroorotate + H(2)O = N-carbamoyl-L-aspartate.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Pathway

Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 3/6.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the DHOase family. Type 2 subfamily.

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Ontologies

Keywords

   Biological processPyrimidine biosynthesis
   LigandMetal-binding
Zinc
   Molecular functionHydrolase
   Technical termComplete proteome

Gene Ontology (GO)

   Biological processpyrimidine nucleotide biosynthetic process

Inferred from electronic annotation. Source: HAMAP

   Molecular functiondihydroorotase activity

Inferred from electronic annotation. Source: HAMAP

zinc ion binding

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 425425Dihydroorotase
PRO_1000071755

Sites

Metal binding561Zinc 1 By similarity
Metal binding581Zinc 1 By similarity
Metal binding1381Zinc 1; via carbamate group By similarity
Metal binding1381Zinc 2; via carbamate group By similarity
Metal binding1751Zinc 2 By similarity
Metal binding2281Zinc 2 By similarity
Metal binding3011Zinc 1 By similarity

Amino acid modifications

Modified residue1381N6-carboxylysine By similarity

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Sequences

Sequence LengthMass (Da)Tools
A8YVZ5-1 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: 187D96B072910B33

FASTA42546,292
        10         20         30         40         50         60 
MQTVIKNGTV YQNGRLIHAD VLIEDQKIKA IGTDLTGDKV IDATGKLVSP GLVDVHVHYR 

        70         80         90        100        110        120 
DPGQTYKEDI ETGSKAAAHG GFTTVGAMPN VTPVPDTPDL MKKMVQENKQ KGIVHIFQYG 

       130        140        150        160        170        180 
PITKNETTDE LPDYAALKKA GAFALSNDGH GVQTAQTMYL AMQEAKKNDL IVAAHAQDDS 

       190        200        210        220        230        240 
LFNHGIVNEG EKAKELNLPP VTELAETTQI ARDLLLAEKT GVHYHICHVS TKTSVELVRM 

       250        260        270        280        290        300 
AKACGINVTC EAAPHHLLLT EDDIPKDNGY YKMNPPLRSK EDQAALLVGL LDGTIDLIAT 

       310        320        330        340        350        360 
DHAPHAKQEK QGGMQNAAFG ITGSETAFST LYTKFVKEDK VFTLEQLLSW LSDQPAKVFG 

       370        380        390        400        410        420 
LKKAGVLEPG CPADVAIFDL EHETELKEKD YQSKGINTPF TGQKIYGATV MTMVDGEVVY 


QRGEK

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References

[1]"Genome sequence of Lactobacillus helveticus: an organism distinguished by selective gene loss and IS element expansion."
Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K., McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F., Beresford T., Ross R.P.
J. Bacteriol. 190:727-735(2008) [PubMed: 17993529] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

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Cross-references

Sequence databases

CP000517 Genomic DNA. Translation: ABX27424.1.
RefSeqYP_001577723.1.

3D structure databases

ModBaseSearch...

Protein family/group databases

MEROPSM38.972.

Genome annotation databases

GeneID5771450.
GenomeReviewsGene locus lhv_1445 in contig CP000517_GR.
KEGGlhe:lhv_1445.

Organism-specific databases

CMRSearch...

Family and domain databases

HAMAPMF_00220.
[Tree]
InterProIPR006680. Amidohydro_1.
IPR004722. DHOmult.
IPR002195. Dihydroorotase_CS.
[Graphical view]
PfamPF01979. Amidohydro_1. 1 hit.
[Graphical view]
ProDomPD000518. DHOase. 1 hit.
[Graphical view] [Entries sharing at least one domain]
TIGRFAMsTIGR00857. pyrC_multi. 1 hit.
PROSITEPS00482. DIHYDROOROTASE_1. False negative.
PS00483. DIHYDROOROTASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePYRC_LACH4
AccessionPrimary (citable) accession number: A8YVZ5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 15, 2008
Last modified: September 2, 2008
This is version 7 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents