ID   TRMFO_LACH4             Reviewed;         407 AA.
AC   A8YV48;
DT   02-SEP-2008, integrated into UniProtKB/Swiss-Prot.
DT   15-JAN-2008, sequence version 1.
DT   16-JUN-2009, entry version 13.
DE   RecName: Full=Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO;
DE            EC=2.1.1.74;
DE   AltName: Full=Folate-dependent tRNA (uracil-5-)-methyltransferase;
DE   AltName: Full=Folate-dependent tRNA(M-5-U54)-methyltransferase;
GN   Name=trmFO; OrderedLocusNames=lhv_1075;
OS   Lactobacillus helveticus (strain DPC 4571).
OC   Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=405566;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=17993529; DOI=10.1128/JB.01295-07;
RA   Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K.,
RA   McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F.,
RA   Beresford T., Ross R.P.;
RT   "Genome sequence of Lactobacillus helveticus: an organism
RT   distinguished by selective gene loss and IS element expansion.";
RL   J. Bacteriol. 190:727-735(2008).
CC   -!- FUNCTION: Catalyzes the folate-dependent formation of 5-methyl-
CC       uridine at position 54 (M-5-U54) in all tRNAs (By similarity).
CC   -!- CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + tRNA
CC       containing uridine at position 54 + FADH(2) = tetrahydrofolate +
CC       tRNA containing ribothymidine at position 54 + FAD.
CC   -!- COFACTOR: FAD (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the mnmG family. TrmFO subfamily.
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DR   EMBL; CP000517; ABX27136.1; -; Genomic_DNA.
DR   RefSeq; YP_001577427.1; -.
DR   GeneID; 5771781; -.
DR   GenomeReviews; CP000517_GR; lhv_1075.
DR   KEGG; lhe:lhv_1075; -.
DR   OMA; A8YV48; MKPVGLT.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0050660; F:FAD binding; IEA:HAMAP.
DR   GO; GO:0047151; F:methylenetetrahydrofolate-tRNA-(uracil-5-)-...; IEA:EC.
DR   GO; GO:0009021; F:tRNA (uracil-5-)-methyltransferase activity; IEA:HAMAP.
DR   GO; GO:0008033; P:tRNA processing; IEA:HAMAP.
DR   HAMAP; MF_01037; -; 1.
DR   InterPro; IPR004417; Gid.
DR   InterPro; IPR002218; GIDA-rel.
DR   InterPro; IPR016040; NAD(P)-bd_dom.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   PANTHER; PTHR11806; GIDA; 1.
DR   Pfam; PF01134; GIDA; 1.
DR   ProDom; PD003738; GIDA; 1.
DR   TIGRFAMs; TIGR00137; gid_trmFO; 1.
DR   PROSITE; PS01280; GIDA_1; FALSE_NEG.
DR   PROSITE; PS01281; GIDA_2; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; FAD; Flavoprotein; Methyltransferase;
KW   Transferase; tRNA processing.
FT   CHAIN         1    407       Methylenetetrahydrofolate--tRNA-(uracil-
FT                                5-)-methyltransferase trmFO.
FT                                /FTId=PRO_0000346347.
FT   NP_BIND       9     14       FAD (By similarity).
SQ   SEQUENCE   407 AA;  44505 MW;  F48EA9357797715A CRC64;
     MPKNVTVIGA GLAGSEATWQ LAKRGIHVDL YEMRPQKETP AHETGEFAEL VCTNSMRSNQ
     LSNAVGLLKE EMRHLDSLIM KAADKTQVPA GGALAVDRDS FSKYVTDTLR GLDNVTVHEE
     EITEIPEDGI TIIATGPLTS DALAEQIQKF SGTDSLHFFD AAAPIVAADS IDMNIVYKKS
     RYDRGEAAYL NCPMNKEQYE NFTRELIKAE TAQLHGFEKN DVFEGCMPIE VMAARGAKTM
     LFGPLKPVGL EDPHTGETPY AVVQLRQDNA AASMYNIVGF QTHLKYGEQK RVFSMIPGLE
     NARFVRYGKM HRNTYMASPD VLTASYEAKK RPGLFFAGQM TGVEGYVESA GSGLVAGVNA
     AREALGEEPI AFPKDTALGS MANYVTTTSA KHFPPMNASL HFYLLGK
//