Reviewed, UniProtKB/Swiss-Prot A8YV48 (TRMFO_LACH4)

Names and origin

Protein names

Recommended name:
    Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO
    EC=2.1.1.74
Alternative name(s):
    Folate-dependent tRNA (uracil-5-)-methyltransferase
    Folate-dependent tRNA(M-5-U54)-methyltransferase

Gene names

Name:	trmFO
Ordered Locus Names:	lhv_1075

Organism

Lactobacillus helveticus (strain DPC 4571) [Complete proteome] [HAMAP]

Taxonomic identifier

405566 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	407 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs By similarity.
Catalytic activity	5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH2 = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD. HAMAP MF_01037
Cofactor	FAD By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the mnmG family. TrmFO subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	FAD binding Inferred from electronic annotation. Source: HAMAP methylenetetrahydrofolate-tRNA-(uracil-5-)-methyltransferase (FADH2-oxidizing) activity Inferred from electronic annotation. Source: EC tRNA (uracil-5-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 407	407	Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase trmFO HAMAP MF_01037		PRO_0000346347
Regions
	Nucleotide binding	9 – 14	6	FAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8YV48-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: F48EA9357797715A	FASTA	407	44,505
	10 20 30 40 50 60 MPKNVTVIGA GLAGSEATWQ LAKRGIHVDL YEMRPQKETP AHETGEFAEL VCTNSMRSNQ 70 80 90 100 110 120 LSNAVGLLKE EMRHLDSLIM KAADKTQVPA GGALAVDRDS FSKYVTDTLR GLDNVTVHEE 130 140 150 160 170 180 EITEIPEDGI TIIATGPLTS DALAEQIQKF SGTDSLHFFD AAAPIVAADS IDMNIVYKKS 190 200 210 220 230 240 RYDRGEAAYL NCPMNKEQYE NFTRELIKAE TAQLHGFEKN DVFEGCMPIE VMAARGAKTM 250 260 270 280 290 300 LFGPLKPVGL EDPHTGETPY AVVQLRQDNA AASMYNIVGF QTHLKYGEQK RVFSMIPGLE 310 320 330 340 350 360 NARFVRYGKM HRNTYMASPD VLTASYEAKK RPGLFFAGQM TGVEGYVESA GSGLVAGVNA 370 380 390 400 AREALGEEPI AFPKDTALGS MANYVTTTSA KHFPPMNASL HFYLLGK

References

[1]

"Genome sequence of Lactobacillus helveticus: an organism distinguished by selective gene loss and IS element expansion." Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K., McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F., Beresford T., Ross R.P. J. Bacteriol. 190:727-735(2008) [PubMed: 17993529] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000517 Genomic DNA. Translation: ABX27136.1.
RefSeq	YP_001577427.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	5771781.
GenomeReviews	Gene locus lhv_1075 in contig CP000517_GR.
KEGG	lhe:lhv_1075.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	A8YV48. MKPVGLT.
Family and domain databases
HAMAP	MF_01037. [Tree]
InterPro	IPR004417. Gid. IPR002218. GIDA-rel. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR11806. GIDA. 1 hit.
Pfam	PF01134. GIDA. 1 hit. [Graphical view]
ProDom	PD003738. GIDA. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00137. gid_trmFO. 1 hit.
PROSITE	PS01280. GIDA_1. False negative. PS01281. GIDA_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TRMFO_LACH4

Accession

Primary (citable) accession number: A8YV48

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	January 15, 2008
Last modified:	June 16, 2009
This is version 13 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)