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A8YUW0 (TYSY_LACH4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 44. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:lhv_0955
OrganismLactobacillus helveticus (strain DPC 4571) [Complete proteome] [HAMAP]
Taxonomic identifier405566 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length318 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 318318Thymidylate synthase HAMAP-Rule MF_00008
PRO_1000070925

Sites

Active site2001 By similarity

Sequences

Sequence LengthMass (Da)Tools
A8YUW0 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: D0BDE5676277A7AA

FASTA31836,882
        10         20         30         40         50         60 
MAILEQPYLD LLQKIMSEGH GKDDRTGTGT RSYFGAQMRF DLSQGFPLLT TKKVPFGLIK 

        70         80         90        100        110        120 
SELLWFLRGD TNIRFLLEHN NHIWDEWAFK NWVNSSEYQG PDMTDFGLRS QSDPEFNKIY 

       130        140        150        160        170        180 
QAEMKKFDQR ILDDEDFAKK YGNLGDVYGA QWRHWEKREG GFIDQIADVI KQIKETPDSR 

       190        200        210        220        230        240 
RMIVTAWNPE DVPTSALPPC HVMFQFYVVD GKISVQLYQR SGDMFLGVPF NIASYSLLLN 

       250        260        270        280        290        300 
LIARETGLQV GEFIHTLGDA HIYRNHLKQV EELLSRKPYD SPQLWLNPEK KNIADFEMKD 

       310 
IKVVDYQHHG TIKAPVAV 

« Hide

References

[1]"Genome sequence of Lactobacillus helveticus: an organism distinguished by selective gene loss and IS element expansion."
Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K., McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F., Beresford T., Ross R.P.
J. Bacteriol. 190:727-735(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DPC 4571.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000517 Genomic DNA. Translation: ABX27048.1.
RefSeqYP_001577339.1. NC_010080.1.

3D structure databases

ProteinModelPortalA8YUW0.
SMRA8YUW0. Positions 3-318.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING405566.lhv_0955.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX27048; ABX27048; lhv_0955.
GeneID5771864.
KEGGlhe:lhv_0955.
PATRIC22234087. VBILacHel91643_0996.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257899.
KOK00560.
OMAAQWRHWE.
OrthoDBEOG6K6V53.

Enzyme and pathway databases

BioCycLHEL405566:GJEN-886-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 2 hits.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_LACH4
AccessionPrimary (citable) accession number: A8YUW0
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 15, 2008
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways