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A8YUP7

- SYI_LACH4

UniProt

A8YUP7 - SYI_LACH4

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Protein
Isoleucine--tRNA ligase
Gene
ileS, lhv_0863
Organism
Lactobacillus helveticus (strain DPC 4571)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei553 – 5531Aminoacyl-adenylate By similarity
Binding sitei597 – 5971ATP By similarity
Metal bindingi886 – 8861Zinc By similarity
Metal bindingi889 – 8891Zinc By similarity
Metal bindingi906 – 9061Zinc By similarity
Metal bindingi909 – 9091Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciLHEL405566:GJEN-808-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:lhv_0863
OrganismiLactobacillus helveticus (strain DPC 4571)
Taxonomic identifieri405566 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000790: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 927927Isoleucine--tRNA ligaseUniRule annotation
PRO_1000073710Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi405566.lhv_0863.

Structurei

3D structure databases

ProteinModelPortaliA8YUP7.
SMRiA8YUP7. Positions 1-915.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi594 – 5985"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiLGHRVHY.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8YUP7-1 [UniParc]FASTAAdd to Basket

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MKIKDTLNLG KTKFKMRGNL PVREAEWQKQ WRENKLYEQR LKLNEGKPRF    50
DLHDGPPFAN GNIHMGHALN KISKDIIVRY KNMNGYYAPY VPGWDTHGLP 100
VEQQLAKKGV DRKTMDRAKY RELCRQYAEE QVQKQMTDFK RLGVMADWDH 150
PYITLQHDFE AQEIRVFGEM FKKGYIYKGK KPVYWSWSSE STLAEAEVEY 200
KDVEANSIFV AFPVTDGKGI IDPKDTYFVI WTTTPWTIPA NEAICVNPKF 250
DYSVVQVGDK RYVVASGLLD KVAEEIGWDD YKVVQTVKGA DMEYMKAKHP 300
IYDKESLVIE GFHVTLDDGT GLVHTAPGFG EDDFNVGQRY NLPIFSPVDA 350
HGCYTDEVPE LKGMFYQDVD KLMVQKLKDA GALLKLKIFT HSYPHDWRTK 400
KPVIFRATTQ WFASIAPFRD QILDQIEKAS FIPSWGKTRL YNMIKDRGDW 450
VISRQRAWGV PLPIFYAEDG TPIVTPETIE HIAKIFEKEG SNAWYTHTAK 500
ELLPEGFTSE HSPNGEFTKE KDILDVWFDS GSSWSGVMEK RDGLHYPADL 550
YLEGSDQYRG WFNSSLITSV AVTGQAPYKQ VLSQGFVLDD KGHKMSKSLG 600
NVISPNDVIK KMGAEIIRLW VAQADTTSDV AVSMGILQQS AESYRKIRNT 650
FRYMLANTSD FDPKENRVAY DDLRSVDQYM EVKLNDLIKE CLAAYDKFDF 700
TTVFKKVFNF ISNDLSAFYL DFAKDVLYIE VENSHARRSM QTVIYDAAVK 750
LAKILTPILP HTMEEIWGFL KEDEDYVQLA NMPEVENYAN HDELLENWAK 800
FMKLRDDVLK ALEDARNKKL IGKSFEAAVT IYPDKETKAM LDELDADFRE 850
ILIVSKLTIS DDEAPVDAEQ LANAAVVVKH AEGEVCPRCR MIRTDIGADP 900
KLPMLCGRCA EIVEANYPEV VQEGLEK 927
Length:927
Mass (Da):106,225
Last modified:January 15, 2008 - v1
Checksum:i820EF079C2C2EC87
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000517 Genomic DNA. Translation: ABX26985.1.
RefSeqiYP_001577276.1. NC_010080.1.

Genome annotation databases

EnsemblBacteriaiABX26985; ABX26985; lhv_0863.
GeneIDi5771930.
KEGGilhe:lhv_0863.
PATRICi22233900. VBILacHel91643_0904.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000517 Genomic DNA. Translation: ABX26985.1 .
RefSeqi YP_001577276.1. NC_010080.1.

3D structure databases

ProteinModelPortali A8YUP7.
SMRi A8YUP7. Positions 1-915.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 405566.lhv_0863.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABX26985 ; ABX26985 ; lhv_0863 .
GeneIDi 5771930.
KEGGi lhe:lhv_0863.
PATRICi 22233900. VBILacHel91643_0904.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi LGHRVHY.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci LHEL405566:GJEN-808-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of Lactobacillus helveticus: an organism distinguished by selective gene loss and IS element expansion."
    Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K., McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F., Beresford T., Ross R.P.
    J. Bacteriol. 190:727-735(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DPC 4571.

Entry informationi

Entry nameiSYI_LACH4
AccessioniPrimary (citable) accession number: A8YUP7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 15, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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