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A8YUP7 (SYI_LACH4) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Isoleucine--tRNA ligase

EC=6.1.1.5
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name=IleRS
Gene names
Name:ileS
Ordered Locus Names:lhv_0863
OrganismLactobacillus helveticus (strain DPC 4571) [Complete proteome] [HAMAP]
Taxonomic identifier405566 [NCBI]
Taxonomic lineageBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus

Protein attributes

Sequence length927 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity. HAMAP-Rule MF_02002

Catalytic activity

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile). HAMAP-Rule MF_02002

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP-Rule MF_02002

Subunit structure

Monomer By similarity. HAMAP-Rule MF_02002

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_02002.

Domain

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity. HAMAP-Rule MF_02002

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processisoleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

isoleucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 927927Isoleucine--tRNA ligase HAMAP-Rule MF_02002
PRO_1000073710

Regions

Motif57 – 6711"HIGH" region HAMAP-Rule MF_02002
Motif594 – 5985"KMSKS" region HAMAP-Rule MF_02002

Sites

Metal binding8861Zinc By similarity
Metal binding8891Zinc By similarity
Metal binding9061Zinc By similarity
Metal binding9091Zinc By similarity
Binding site5531Aminoacyl-adenylate By similarity
Binding site5971ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8YUP7 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: 820EF079C2C2EC87

FASTA927106,225
        10         20         30         40         50         60 
MKIKDTLNLG KTKFKMRGNL PVREAEWQKQ WRENKLYEQR LKLNEGKPRF DLHDGPPFAN 

        70         80         90        100        110        120 
GNIHMGHALN KISKDIIVRY KNMNGYYAPY VPGWDTHGLP VEQQLAKKGV DRKTMDRAKY 

       130        140        150        160        170        180 
RELCRQYAEE QVQKQMTDFK RLGVMADWDH PYITLQHDFE AQEIRVFGEM FKKGYIYKGK 

       190        200        210        220        230        240 
KPVYWSWSSE STLAEAEVEY KDVEANSIFV AFPVTDGKGI IDPKDTYFVI WTTTPWTIPA 

       250        260        270        280        290        300 
NEAICVNPKF DYSVVQVGDK RYVVASGLLD KVAEEIGWDD YKVVQTVKGA DMEYMKAKHP 

       310        320        330        340        350        360 
IYDKESLVIE GFHVTLDDGT GLVHTAPGFG EDDFNVGQRY NLPIFSPVDA HGCYTDEVPE 

       370        380        390        400        410        420 
LKGMFYQDVD KLMVQKLKDA GALLKLKIFT HSYPHDWRTK KPVIFRATTQ WFASIAPFRD 

       430        440        450        460        470        480 
QILDQIEKAS FIPSWGKTRL YNMIKDRGDW VISRQRAWGV PLPIFYAEDG TPIVTPETIE 

       490        500        510        520        530        540 
HIAKIFEKEG SNAWYTHTAK ELLPEGFTSE HSPNGEFTKE KDILDVWFDS GSSWSGVMEK 

       550        560        570        580        590        600 
RDGLHYPADL YLEGSDQYRG WFNSSLITSV AVTGQAPYKQ VLSQGFVLDD KGHKMSKSLG 

       610        620        630        640        650        660 
NVISPNDVIK KMGAEIIRLW VAQADTTSDV AVSMGILQQS AESYRKIRNT FRYMLANTSD 

       670        680        690        700        710        720 
FDPKENRVAY DDLRSVDQYM EVKLNDLIKE CLAAYDKFDF TTVFKKVFNF ISNDLSAFYL 

       730        740        750        760        770        780 
DFAKDVLYIE VENSHARRSM QTVIYDAAVK LAKILTPILP HTMEEIWGFL KEDEDYVQLA 

       790        800        810        820        830        840 
NMPEVENYAN HDELLENWAK FMKLRDDVLK ALEDARNKKL IGKSFEAAVT IYPDKETKAM 

       850        860        870        880        890        900 
LDELDADFRE ILIVSKLTIS DDEAPVDAEQ LANAAVVVKH AEGEVCPRCR MIRTDIGADP 

       910        920 
KLPMLCGRCA EIVEANYPEV VQEGLEK 

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References

[1]"Genome sequence of Lactobacillus helveticus: an organism distinguished by selective gene loss and IS element expansion."
Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K., McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F., Beresford T., Ross R.P.
J. Bacteriol. 190:727-735(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DPC 4571.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000517 Genomic DNA. Translation: ABX26985.1.
RefSeqYP_001577276.1. NC_010080.1.

3D structure databases

ProteinModelPortalA8YUP7.
SMRA8YUP7. Positions 1-915.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING405566.lhv_0863.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABX26985; ABX26985; lhv_0863.
GeneID5771930.
KEGGlhe:lhv_0863.
PATRIC22233900. VBILacHel91643_0904.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0060.
HOGENOMHOG000246402.
KOK01870.
OMALGHRVHY.
OrthoDBEOG644ZM1.

Enzyme and pathway databases

BioCycLHEL405566:GJEN-808-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_02002. Ile_tRNA_synth_type1.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSPR00984. TRNASYNTHILE.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00392. ileS. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYI_LACH4
AccessionPrimary (citable) accession number: A8YUP7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 15, 2008
Last modified: May 14, 2014
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries