Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

NAD kinase

Gene

nadK

Organism
Lactobacillus helveticus (strain DPC 4571)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

Catalytic activityi

ATP + NAD+ = ADP + NADP+.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei45 – 451Proton acceptorUniRule annotation
Binding sitei147 – 1471NADUniRule annotation
Binding sitei149 – 1491NADUniRule annotation
Binding sitei184 – 1841NAD; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi45 – 462NADUniRule annotation
Nucleotide bindingi121 – 1222NADUniRule annotation
Nucleotide bindingi160 – 1656NADUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Ligandi

ATP-binding, NAD, NADP, Nucleotide-binding

Enzyme and pathway databases

BioCyciLHEL405566:GJEN-651-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
Alternative name(s):
ATP-dependent NAD kinaseUniRule annotation
Gene namesi
Name:nadKUniRule annotation
Ordered Locus Names:lhv_0694
OrganismiLactobacillus helveticus (strain DPC 4571)
Taxonomic identifieri405566 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliLactobacillalesLactobacillaceaeLactobacillus
ProteomesiUP000000790 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 270270NAD kinasePRO_1000072084Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi405566.lhv_0694.

Structurei

3D structure databases

ProteinModelPortaliA8YUA3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD kinase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
KOiK00858.
OMAiHRYENQV.
OrthoDBiEOG6PZXDR.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.

Sequencei

Sequence statusi: Complete.

A8YUA3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVTIAHNNY DKTLETVAYL KKLLQKKNVI FDAKYPDIVI TVGGDGTLIN
60 70 80 90 100
AFHRYENQVD SVRFIGVHTG HLGFYTDWRN YDIDKMVDAL LLTKGQPAKY
110 120 130 140 150
PLLEIKMLTE SGDTRYHLAV NESAVKRISH TLEADVYIDD ELFENFRGDG
160 170 180 190 200
LCVSTPTGST AYSKSLGGAV IHPRLKALQM TEIASINNRV FRTLSAPIVI
210 220 230 240 250
APDQWITIVP NVDHFVMTVD GARIDVRNAK KIEYRISKHS IQFDQFGHHH
260 270
FWSRVQNAFI KDTEDNDKFI
Length:270
Mass (Da):30,742
Last modified:January 15, 2008 - v1
Checksum:i4A6B3B1B687F93B6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000517 Genomic DNA. Translation: ABX26841.1.
RefSeqiWP_012211595.1. NC_010080.1.
YP_001577132.1. NC_010080.1.

Genome annotation databases

EnsemblBacteriaiABX26841; ABX26841; lhv_0694.
KEGGilhe:lhv_0694.
PATRICi22233529. VBILacHel91643_0722.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000517 Genomic DNA. Translation: ABX26841.1.
RefSeqiWP_012211595.1. NC_010080.1.
YP_001577132.1. NC_010080.1.

3D structure databases

ProteinModelPortaliA8YUA3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi405566.lhv_0694.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABX26841; ABX26841; lhv_0694.
KEGGilhe:lhv_0694.
PATRICi22233529. VBILacHel91643_0722.

Phylogenomic databases

eggNOGiCOG0061.
HOGENOMiHOG000275803.
KOiK00858.
OMAiHRYENQV.
OrthoDBiEOG6PZXDR.

Enzyme and pathway databases

BioCyciLHEL405566:GJEN-651-MONOMER.

Family and domain databases

Gene3Di2.60.200.30. 1 hit.
3.40.50.10330. 1 hit.
HAMAPiMF_00361. NAD_kinase.
InterProiIPR017438. ATP-NAD_kinase_dom_1.
IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
IPR016064. NAD/diacylglycerol_kinase.
IPR002504. NADK.
[Graphical view]
PANTHERiPTHR20275. PTHR20275. 1 hit.
PfamiPF01513. NAD_kinase. 1 hit.
[Graphical view]
SUPFAMiSSF111331. SSF111331. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of Lactobacillus helveticus: an organism distinguished by selective gene loss and IS element expansion."
    Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K., McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F., Beresford T., Ross R.P.
    J. Bacteriol. 190:727-735(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DPC 4571.

Entry informationi

Entry nameiNADK_LACH4
AccessioniPrimary (citable) accession number: A8YUA3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: January 15, 2008
Last modified: May 27, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.