Reviewed, UniProtKB/Swiss-Prot A8YTK7 (ASNA_LACH4)

Names and origin

Protein names

Recommended name:
    Aspartate--ammonia ligase
    EC=6.3.1.1
Alternative name(s):
    Asparagine synthetase A

Gene names

Name:	asnA
Ordered Locus Names:	lhv_2034

Organism

Lactobacillus helveticus (strain DPC 4571) [Complete proteome] [HAMAP]

Taxonomic identifier

405566 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	337 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + NH3 = AMP + diphosphate + L-asparagine. HAMAP MF_00555
Pathway	Amino-acid biosynthesis; L-asparagine biosynthesis; L-asparagine from L-aspartate (ammonia route): step 1/1. HAMAP MF_00555
Subcellular location	Cytoplasm By similarity HAMAP MF_00555.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. AsnA subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Asparagine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	asparagine biosynthetic process Inferred from electronic annotation. Source: HAMAP tRNA aminoacylation for protein translation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aminoacyl-tRNA ligase activity Inferred from electronic annotation. Source: InterPro aspartate-ammonia ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 337	337	Aspartate--ammonia ligase HAMAP MF_00555		PRO_1000072560

Sequences

	Sequence		Length	Mass (Da)
	A8YTK7-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: F061389FA507E426	FASTA	337	38,900
	10 20 30 40 50 60 MTLILPKDYR PTLTVRDTEA AIVFIRETFQ DKLASILGVQ RMSAPMFVEQ KTGLNDNLNG 70 80 90 100 110 120 VERPVAFDMK AMPKEDTIEI VHSLAKWKRL ALKKYGFGIH EGLYTNMNAI RRDEDLDNFH 130 140 150 160 170 180 SIYVDQWDWE KIIRKEERNT DTLEITVNKI FRAIKETEKL VSARHPGSTY RLGDHVSFIT 190 200 210 220 230 240 TQELEDRWPD LSPDERENQI AKEEKVVFIM KIGDKLKRSG KPHDGRAPDY DDWQLNGDLV 250 260 270 280 290 300 FWYEPLQSKI EISSMGIRVS EESLKEQLKK AHCEERAKLP FHQMILKGEL PYSIGGGIGQ 310 320 330 SRLCMLLLGK AHIGEVQASI WPEDMVEKCE ADNIELL

References

[1]

"Genome sequence of Lactobacillus helveticus: an organism distinguished by selective gene loss and IS element expansion." Callanan M., Kaleta P., O'Callaghan J., O'Sullivan O., Jordan K., McAuliffe O., Sangrador-Vegas A., Slattery L., Fitzgerald G.F., Beresford T., Ross R.P. J. Bacteriol. 190:727-735(2008) [PubMed: 17993529] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000517 Genomic DNA. Translation: ABX27831.1.
RefSeq	YP_001578139.1.
3D structure databases
SMR	A8YTK7. Positions 16-331.
ModBase	Search...
Genome annotation databases
GeneID	5771478.
GenomeReviews	Gene locus lhv_2034 in contig CP000517_GR.
KEGG	lhe:lhv_2034.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG288146.
OMA	LNDNLNG.
Family and domain databases
HAMAP	MF_00555. AsnA. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-dom. IPR004618. AsnA. [Graphical view]
Pfam	PF03590. AsnA. 1 hit. [Graphical view]
PIRSF	PIRSF001555. Asp_ammon_ligase. 1 hit.
TIGRFAMs	TIGR00669. asnA. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ASNA_LACH4

Accession

Primary (citable) accession number: A8YTK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 26, 2008
Last sequence update:	January 15, 2008
Last modified:	February 9, 2010
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)