Reviewed, UniProtKB/Swiss-Prot A8Y432 (KMO_CAEBR)

Names and origin

Protein names

Recommended name:
    Kynurenine 3-monooxygenase
    EC=1.14.13.9
Alternative name(s):
    Kynurenine 3-hydroxylase

Gene names

ORF Names:

CBG23368

Organism

Caenorhabditis briggsae [Complete proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	461 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid By similarity.
Catalytic activity	L-kynurenine + NADPH + O2 = 3-hydroxy-L-kynurenine + NADP+ + H2O.
Cofactor	FAD By similarity.
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; quinolinate from L-kynurenine: step 1/3.
Subcellular location	Mitochondrion By similarity. Membrane; Multi-pass membrane protein Potential.
Sequence similarities	Belongs to the aromatic-ring hydroxylase family. KMO subfamily.

Ontologies

Keywords
Biological process	Pyridine nucleotide biosynthesis
Cellular component	Membrane Mitochondrion
Domain	Transmembrane
Ligand	FAD Flavoprotein NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD metabolic process Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW pyridine nucleotide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrial outer membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	kynurenine 3-monooxygenase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 461	461	Kynurenine 3-monooxygenase		PRO_0000361910
Regions
	Transmembrane	395 – 415	21	Potential
	Transmembrane	432 – 461	30	Potential

Sequences

	Sequence		Length	Mass (Da)
	A8Y432-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 719D25F54A5B0B46	FASTA	461	52,555
	10 20 30 40 50 60 MPSVAIAGAG LVGALNACFF AQKGWDVSVY EFRKDIRTMK HVQGRSINLA LSQRGKSALE 70 80 90 100 110 120 AVGLKEYIVN QGVPLYARLV HNKDGKTYSR QPYGKPGEHI VSINRRHLNE VMITQAEKSP 130 140 150 160 170 180 NVKFFFEHKV KSVDYDKKQL VVQCTSQPSR IPTFGTKSPP AEHEEFHVEA DLIIACDGAY 190 200 210 220 230 240 SAVRRSLMTI PRFDFSQEYI EHGYVELNIM ANNNEFAFEE NVFHLWPRGH FTLIALANRD 250 260 270 280 290 300 KTFTVTIFAP FTEFEKHMST TEEVLSFFEE NFPDAYLLLG KEHIADTFNR VKPQSLVSIK 310 320 330 340 350 360 CSPHSFFNNL VLMGDAAHAM VPFYGQGMNC GFEDCLVFSE TLEEQNNDIA SAVQVYSERR 370 380 390 400 410 420 VNDAHTINDL AMYNYEELKD LVNKNSYKLR KKFDGFMNAI FPKSWIPLYS MVTFTRIPYS 430 440 450 460 EVVDRRRKQD RILSNLWKTT STLALIGAAI GIYSNRGRLG L

References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed: 14624247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CAAC02000606 Genomic DNA. Translation: CAP39652.1.
3D structure databases
ModBase	Search...
Organism-specific databases
WormBase	WBGene00041735. CBG23368.
Phylogenomic databases
OMA	HTDVLAD.
Family and domain databases
InterPro	IPR002938. mOase_FAD_bd. IPR003042. Rng_hydrolase-like. [Graphical view]
Pfam	PF01494. FAD_binding_3. 1 hit. [Graphical view]
PRINTS	PR00420. RNGMNOXGNASE.
ProtoNet	Search...

Entry information

Entry name

KMO_CAEBR

Accession

Primary (citable) accession number: A8Y432

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 10, 2009
Last sequence update:	January 15, 2008
Last modified:	November 3, 2009
This is version 18 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project