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A8Y236

- GLT10_CAEBR

UniProt

A8Y236 - GLT10_CAEBR

Protein

Putative polypeptide N-acetylgalactosaminyltransferase 10

Gene

gly-10

Organism
Caenorhabditis briggsae
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 2 (13 Oct 2009)
      Previous versions | rss
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    Functioni

    May catalyze the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.By similarity

    Catalytic activityi

    UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

    Cofactori

    Manganese.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei204 – 2041SubstrateBy similarity
    Binding sitei236 – 2361SubstrateBy similarity
    Metal bindingi259 – 2591ManganeseBy similarity
    Metal bindingi261 – 2611ManganeseBy similarity
    Binding sitei362 – 3621SubstrateBy similarity
    Metal bindingi390 – 3901ManganeseBy similarity
    Binding sitei393 – 3931SubstrateBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC

    GO - Biological processi

    1. protein glycosylation Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Ligandi

    Lectin, Manganese, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00378.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative polypeptide N-acetylgalactosaminyltransferase 10 (EC:2.4.1.41)
    Short name:
    pp-GaNTase 10
    Alternative name(s):
    Protein-UDP acetylgalactosaminyltransferase 10
    UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10
    Gene namesi
    Name:gly-10By similarity
    ORF Names:CBG22373
    OrganismiCaenorhabditis briggsae
    Taxonomic identifieri6238 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000008549: Chromosome IV, UP000008549: Unassembled WGS sequence

    Organism-specific databases

    WormBaseiCBG22373; CBP28929; WBGene00040946; Cbr-gly-10.

    Subcellular locationi

    Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 629629Putative polypeptide N-acetylgalactosaminyltransferase 10PRO_0000328999Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi143 – 1431N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi154 ↔ 385PROSITE-ProRule annotation
    Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi376 ↔ 454PROSITE-ProRule annotation
    Disulfide bondi493 ↔ 510PROSITE-ProRule annotation
    Disulfide bondi539 ↔ 556PROSITE-ProRule annotation
    Disulfide bondi582 ↔ 598PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Interactioni

    Protein-protein interaction databases

    STRINGi6238.CBG22373.

    Structurei

    3D structure databases

    ProteinModelPortaliA8Y236.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini34 – 629596LumenalSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei13 – 3321Helical; Signal-anchor for type II membrane proteinAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini526 – 629104Ricin B-type lectinPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni163 – 275113Catalytic subdomain AAdd
    BLAST
    Regioni331 – 39363Catalytic subdomain BAdd
    BLAST
    Regioni393 – 40614Flexible loopBy similarityAdd
    BLAST

    Domaini

    There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
    The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

    Sequence similaritiesi

    Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.Sequence Analysis
    Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG239675.
    OrthoDBiEOG7J9VP2.

    Family and domain databases

    Gene3Di3.90.550.10. 1 hit.
    InterProiIPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR000772. Ricin_B_lectin.
    [Graphical view]
    PfamiPF00535. Glycos_transf_2. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view]
    SMARTiSM00458. RICIN. 1 hit.
    [Graphical view]
    SUPFAMiSSF50370. SSF50370. 1 hit.
    SSF53448. SSF53448. 1 hit.
    PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8Y236-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGLSRYLSRR HHWVIQYCAL LLFLYFIYSY VAVSNDAPRL NEEIPVFREF    50
    SEDLPQGSRK FPEQKPAAAL GDEALDPFEK YRGHEKIKWE DEVAYEKDKA 100
    REGPGEWGKP VKLPDDKETE KEALSLYKAN GYNAYISDMI SLNRSIKDIR 150
    HKECKKMTYS AKLPTVSVIF PFHEEHNSTL LRSVYSVINR SPPELLKEII 200
    LVDDFSEKPA LRQPLEDFLK KNKIDHIVKI LRTKKREGLI RGRQLGAQEA 250
    TGEILIFLDA HSECNYNWLP PLLDPIADDY RTVVCPFVDV IDCETYEIRP 300
    QDEGARGSFD WAFNYKRLPL TKKDRENPTK PFDSPVMAGG YFAISAKWFW 350
    ELGGYDEGLD IWGGEQYELS FKVWQCHGKM VDAPCSRVAH IYRCKYAPFK 400
    NAGMGDFVSR NYKRVAEVWM DEYKETLYKH RPGIGNADAG DLKLMKGIRE 450
    KLQCKSFDWF MKEIAFDQDK YYPAIEPKAS AEGEIRHVAS NLCIDTQFKE 500
    QNQRFGLRKC ASEDKDGGGE QDLRLTRWHD IRPKGRKICF DVSTSVDKAP 550
    IILFDCHSMK GNQLFKYRMP QKQIYHPVSG QCLTADENGK GFLHMKKCDS 600
    SSKLQQWAWQ TIDQELLDQR QANEHKELE 629
    Length:629
    Mass (Da):73,008
    Last modified:October 13, 2009 - v2
    Checksum:iFCD8FD9F61829238
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HE600912 Genomic DNA. Translation: CAP38976.2.

    Genome annotation databases

    EnsemblMetazoaiCBG22373; CBG22373; CBG22373.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HE600912 Genomic DNA. Translation: CAP38976.2 .

    3D structure databases

    ProteinModelPortali A8Y236.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6238.CBG22373.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai CBG22373 ; CBG22373 ; CBG22373 .

    Organism-specific databases

    WormBasei CBG22373 ; CBP28929 ; WBGene00040946 ; Cbr-gly-10.

    Phylogenomic databases

    eggNOGi NOG239675.
    OrthoDBi EOG7J9VP2.

    Enzyme and pathway databases

    UniPathwayi UPA00378 .

    Family and domain databases

    Gene3Di 3.90.550.10. 1 hit.
    InterProi IPR001173. Glyco_trans_2-like.
    IPR029044. Nucleotide-diphossugar_trans.
    IPR000772. Ricin_B_lectin.
    [Graphical view ]
    Pfami PF00535. Glycos_transf_2. 1 hit.
    PF00652. Ricin_B_lectin. 1 hit.
    [Graphical view ]
    SMARTi SM00458. RICIN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50370. SSF50370. 1 hit.
    SSF53448. SSF53448. 1 hit.
    PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AF16.

    Entry informationi

    Entry nameiGLT10_CAEBR
    AccessioniPrimary (citable) accession number: A8Y236
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 8, 2008
    Last sequence update: October 13, 2009
    Last modified: October 1, 2014
    This is version 45 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3