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A8Y236

- GLT10_CAEBR

UniProt

A8Y236 - GLT10_CAEBR

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Protein

Putative polypeptide N-acetylgalactosaminyltransferase 10

Gene

gly-10

Organism
Caenorhabditis briggsae
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

May catalyze the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

Cofactori

Mn2+By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei204 – 2041SubstrateBy similarity
Binding sitei236 – 2361SubstrateBy similarity
Metal bindingi259 – 2591ManganeseBy similarity
Metal bindingi261 – 2611ManganeseBy similarity
Binding sitei362 – 3621SubstrateBy similarity
Metal bindingi390 – 3901ManganeseBy similarity
Binding sitei393 – 3931SubstrateBy similarity

GO - Molecular functioni

  1. carbohydrate binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. polypeptide N-acetylgalactosaminyltransferase activity Source: UniProtKB-EC

GO - Biological processi

  1. protein glycosylation Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Lectin, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative polypeptide N-acetylgalactosaminyltransferase 10 (EC:2.4.1.41)
Short name:
pp-GaNTase 10
Alternative name(s):
Protein-UDP acetylgalactosaminyltransferase 10
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10
Gene namesi
Name:gly-10By similarity
ORF Names:CBG22373
OrganismiCaenorhabditis briggsae
Taxonomic identifieri6238 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000008549: Chromosome IV, UP000008549: Unassembled WGS sequence

Organism-specific databases

WormBaseiCBG22373; CBP28929; WBGene00040946; Cbr-gly-10.

Subcellular locationi

Golgi apparatus membrane By similarity; Single-pass type II membrane protein By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1212CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei13 – 3321Helical; Signal-anchor for type II membrane proteinAdd
BLAST
Topological domaini34 – 629596LumenalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. Golgi apparatus Source: UniProtKB-KW
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 629629Putative polypeptide N-acetylgalactosaminyltransferase 10PRO_0000328999Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi143 – 1431N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi154 ↔ 385PROSITE-ProRule annotation
Glycosylationi177 – 1771N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi376 ↔ 454PROSITE-ProRule annotation
Disulfide bondi493 ↔ 510PROSITE-ProRule annotation
Disulfide bondi539 ↔ 556PROSITE-ProRule annotation
Disulfide bondi582 ↔ 598PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Protein-protein interaction databases

STRINGi6238.CBG22373.

Structurei

3D structure databases

ProteinModelPortaliA8Y236.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini526 – 629104Ricin B-type lectinPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni163 – 275113Catalytic subdomain AAdd
BLAST
Regioni331 – 39363Catalytic subdomain BAdd
BLAST
Regioni393 – 40614Flexible loopBy similarityAdd
BLAST

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.Sequence Analysis
Contains 1 ricin B-type lectin domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG239675.
InParanoidiA8Y236.
OrthoDBiEOG7J9VP2.

Family and domain databases

Gene3Di3.90.550.10. 1 hit.
InterProiIPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamiPF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTiSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMiSSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEiPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8Y236-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLSRYLSRR HHWVIQYCAL LLFLYFIYSY VAVSNDAPRL NEEIPVFREF
60 70 80 90 100
SEDLPQGSRK FPEQKPAAAL GDEALDPFEK YRGHEKIKWE DEVAYEKDKA
110 120 130 140 150
REGPGEWGKP VKLPDDKETE KEALSLYKAN GYNAYISDMI SLNRSIKDIR
160 170 180 190 200
HKECKKMTYS AKLPTVSVIF PFHEEHNSTL LRSVYSVINR SPPELLKEII
210 220 230 240 250
LVDDFSEKPA LRQPLEDFLK KNKIDHIVKI LRTKKREGLI RGRQLGAQEA
260 270 280 290 300
TGEILIFLDA HSECNYNWLP PLLDPIADDY RTVVCPFVDV IDCETYEIRP
310 320 330 340 350
QDEGARGSFD WAFNYKRLPL TKKDRENPTK PFDSPVMAGG YFAISAKWFW
360 370 380 390 400
ELGGYDEGLD IWGGEQYELS FKVWQCHGKM VDAPCSRVAH IYRCKYAPFK
410 420 430 440 450
NAGMGDFVSR NYKRVAEVWM DEYKETLYKH RPGIGNADAG DLKLMKGIRE
460 470 480 490 500
KLQCKSFDWF MKEIAFDQDK YYPAIEPKAS AEGEIRHVAS NLCIDTQFKE
510 520 530 540 550
QNQRFGLRKC ASEDKDGGGE QDLRLTRWHD IRPKGRKICF DVSTSVDKAP
560 570 580 590 600
IILFDCHSMK GNQLFKYRMP QKQIYHPVSG QCLTADENGK GFLHMKKCDS
610 620
SSKLQQWAWQ TIDQELLDQR QANEHKELE
Length:629
Mass (Da):73,008
Last modified:October 13, 2009 - v2
Checksum:iFCD8FD9F61829238
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE600912 Genomic DNA. Translation: CAP38976.2.

Genome annotation databases

EnsemblMetazoaiCBG22373; CBG22373; CBG22373.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE600912 Genomic DNA. Translation: CAP38976.2 .

3D structure databases

ProteinModelPortali A8Y236.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 6238.CBG22373.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai CBG22373 ; CBG22373 ; CBG22373 .

Organism-specific databases

WormBasei CBG22373 ; CBP28929 ; WBGene00040946 ; Cbr-gly-10.

Phylogenomic databases

eggNOGi NOG239675.
InParanoidi A8Y236.
OrthoDBi EOG7J9VP2.

Enzyme and pathway databases

UniPathwayi UPA00378 .

Family and domain databases

Gene3Di 3.90.550.10. 1 hit.
InterProi IPR001173. Glyco_trans_2-like.
IPR029044. Nucleotide-diphossugar_trans.
IPR000772. Ricin_B_lectin.
[Graphical view ]
Pfami PF00535. Glycos_transf_2. 1 hit.
PF00652. Ricin_B_lectin. 1 hit.
[Graphical view ]
SMARTi SM00458. RICIN. 1 hit.
[Graphical view ]
SUPFAMi SSF50370. SSF50370. 1 hit.
SSF53448. SSF53448. 1 hit.
PROSITEi PS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AF16.

Entry informationi

Entry nameiGLT10_CAEBR
AccessioniPrimary (citable) accession number: A8Y236
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 13, 2009
Last modified: November 26, 2014
This is version 47 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3