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Protein

Putative polypeptide N-acetylgalactosaminyltransferase 10

Gene

gly-10

Organism
Caenorhabditis briggsae
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

May catalyze the initial reaction in O-linked oligosaccharide biosynthesis, the transfer of an N-acetyl-D-galactosamine residue to a serine or threonine residue on the protein receptor.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-galactosamine + polypeptide = UDP + N-acetyl-alpha-D-galactosaminyl-polypeptide.

Cofactori

Mn2+By similarity

Pathwayi: protein glycosylation

This protein is involved in the pathway protein glycosylation, which is part of Protein modification.By similarity
View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei204SubstrateBy similarity1
Binding sitei236SubstrateBy similarity1
Metal bindingi259ManganeseBy similarity1
Metal bindingi261ManganeseBy similarity1
Binding sitei362SubstrateBy similarity1
Metal bindingi390ManganeseBy similarity1
Binding sitei393SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosyltransferase, Transferase
LigandLectin, Manganese, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00378

Names & Taxonomyi

Protein namesi
Recommended name:
Putative polypeptide N-acetylgalactosaminyltransferase 10 (EC:2.4.1.41)
Short name:
pp-GaNTase 10
Alternative name(s):
Protein-UDP acetylgalactosaminyltransferase 10
UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase 10
Gene namesi
Name:gly-10By similarity
ORF Names:CBG22373
OrganismiCaenorhabditis briggsae
Taxonomic identifieri6238 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000008549 Componentsi: Chromosome IV, Unassembled WGS sequence

Organism-specific databases

WormBaseiCBG22373 ; CBP40797 ; WBGene00040946 ; Cbr-gly-10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 12CytoplasmicSequence analysisAdd BLAST12
Transmembranei13 – 33Helical; Signal-anchor for type II membrane proteinAdd BLAST21
Topological domaini34 – 629LumenalSequence analysisAdd BLAST596

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003289991 – 629Putative polypeptide N-acetylgalactosaminyltransferase 10Add BLAST629

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi143N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi154 ↔ 385PROSITE-ProRule annotation
Glycosylationi177N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi376 ↔ 454PROSITE-ProRule annotation
Disulfide bondi493 ↔ 510PROSITE-ProRule annotation
Disulfide bondi539 ↔ 556PROSITE-ProRule annotation
Disulfide bondi582 ↔ 598PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiA8Y236

Interactioni

Protein-protein interaction databases

STRINGi6238.CBG22373

Structurei

3D structure databases

ProteinModelPortaliA8Y236
SMRiA8Y236
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini526 – 629Ricin B-type lectinPROSITE-ProRule annotationAdd BLAST104

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni163 – 275Catalytic subdomain AAdd BLAST113
Regioni331 – 393Catalytic subdomain BAdd BLAST63
Regioni393 – 406Flexible loopBy similarityAdd BLAST14

Domaini

There are two conserved domains in the glycosyltransferase region: the N-terminal domain (domain A, also called GT1 motif), which is probably involved in manganese coordination and substrate binding and the C-terminal domain (domain B, also called Gal/GalNAc-T motif), which is probably involved in catalytic reaction and UDP-Gal binding.By similarity
The ricin B-type lectin domain binds to GalNAc and contributes to the glycopeptide specificity.By similarity

Sequence similaritiesi

Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.Sequence analysis

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3736 Eukaryota
ENOG410XPMK LUCA
InParanoidiA8Y236
OMAiNYRAQDE
OrthoDBiEOG091G085O

Family and domain databases

CDDicd00161 RICIN, 1 hit
Gene3Di3.90.550.10, 1 hit
InterProiView protein in InterPro
IPR001173 Glyco_trans_2-like
IPR029044 Nucleotide-diphossugar_trans
IPR035992 Ricin_B-like_lectins
IPR000772 Ricin_B_lectin
PfamiView protein in Pfam
PF00535 Glycos_transf_2, 1 hit
PF00652 Ricin_B_lectin, 1 hit
SMARTiView protein in SMART
SM00458 RICIN, 1 hit
SUPFAMiSSF50370 SSF50370, 1 hit
SSF53448 SSF53448, 1 hit
PROSITEiView protein in PROSITE
PS50231 RICIN_B_LECTIN, 1 hit

Sequencei

Sequence statusi: Complete.

A8Y236-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGLSRYLSRR HHWVIQYCAL LLFLYFIYSY VAVSNDAPRL NEEIPVFREF
60 70 80 90 100
SEDLPQGSRK FPEQKPAAAL GDEALDPFEK YRGHEKIKWE DEVAYEKDKA
110 120 130 140 150
REGPGEWGKP VKLPDDKETE KEALSLYKAN GYNAYISDMI SLNRSIKDIR
160 170 180 190 200
HKECKKMTYS AKLPTVSVIF PFHEEHNSTL LRSVYSVINR SPPELLKEII
210 220 230 240 250
LVDDFSEKPA LRQPLEDFLK KNKIDHIVKI LRTKKREGLI RGRQLGAQEA
260 270 280 290 300
TGEILIFLDA HSECNYNWLP PLLDPIADDY RTVVCPFVDV IDCETYEIRP
310 320 330 340 350
QDEGARGSFD WAFNYKRLPL TKKDRENPTK PFDSPVMAGG YFAISAKWFW
360 370 380 390 400
ELGGYDEGLD IWGGEQYELS FKVWQCHGKM VDAPCSRVAH IYRCKYAPFK
410 420 430 440 450
NAGMGDFVSR NYKRVAEVWM DEYKETLYKH RPGIGNADAG DLKLMKGIRE
460 470 480 490 500
KLQCKSFDWF MKEIAFDQDK YYPAIEPKAS AEGEIRHVAS NLCIDTQFKE
510 520 530 540 550
QNQRFGLRKC ASEDKDGGGE QDLRLTRWHD IRPKGRKICF DVSTSVDKAP
560 570 580 590 600
IILFDCHSMK GNQLFKYRMP QKQIYHPVSG QCLTADENGK GFLHMKKCDS
610 620
SSKLQQWAWQ TIDQELLDQR QANEHKELE
Length:629
Mass (Da):73,008
Last modified:October 13, 2009 - v2
Checksum:iFCD8FD9F61829238
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE600912 Genomic DNA Translation: CAP38976.2

Similar proteinsi

Entry informationi

Entry nameiGLT10_CAEBR
AccessioniPrimary (citable) accession number: A8Y236
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 8, 2008
Last sequence update: October 13, 2009
Last modified: May 23, 2018
This is version 60 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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