A8Y0L5 (GMD1_CAEBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
GDP-mannose 4,6 dehydratase 1
EC=4.2.1.47

Alternative name(s):
Bacillus thuringiensis toxin-resistant protein 1
Short name=Bt toxin-resistant protein 1
GDP-D-mannose dehydratase
Short name=GMD

Gene names

Name:	bre-1
ORF Names:	CBG21737

Organism

Caenorhabditis briggsae

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Conversion of GDP-D-mannose to GDP-4-keto-6-D-deoxymannose. Involved in susceptibility to pore-forming crystal toxins in conjunction with bre-2, bre-3 and bre-4. Has a role in determining brood size By similarity. UniProtKB Q18801
Catalytic activity	GDP-mannose = GDP-4-dehydro-6-deoxy-D-mannose + H2O. UniProtKB Q18801
Cofactor	NADP By similarity. UniProtKB P93031
Pathway	Nucleotide-sugar biosynthesis; GDP-L-fucose biosynthesis via de novo pathway; GDP-L-fucose from GDP-alpha-D-mannose: step 1/2. UniProtKB Q18801
Sequence similarities	Belongs to the GDP-mannose 4,6-dehydratase family.

Ontologies

Keywords
Biological process	Insecticide resistance
Ligand	NADP
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	GDP-mannose metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	GDP-mannose 4,6-dehydratase activity Inferred from electronic annotation. Source: EC coenzyme binding Inferred from electronic annotation. Source: InterPro nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 377	377	GDP-mannose 4,6 dehydratase 1		PRO_0000324672
Sites
	Active site	161	1	By similarity
	Active site	163	1	Nucleophile By similarity
	Active site	185	1	Nucleophile By similarity
	Binding site	189	1	NADP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8Y0L5 [UniParc]. Last modified September 22, 2009. Version 2. Checksum: 998825857EBA2804	FASTA	377	42,216
	10 20 30 40 50 60 MEGLEACIGQ SHEVMTTPAA ELAAFRARKV ALITGISGQD GSYLAELLLS KGYKVHGIIR 70 80 90 100 110 120 RSSSFNTARI EHLYSNPMTH NGDSSFSLHY GDMTDSSCLI KLISTIEPTE VYHLAAQSHV 130 140 150 160 170 180 KVSFDLPEYT AEVDAVGTLR LLDAIHACRL TEKVRFYQAS TSELYGKVQE IPQSEKTPFY 190 200 210 220 230 240 PRSPYAVAKM YGYWIVVNYR EAYKMFACNG ILFNHESPRR GETFVTRKIT RSVAKISLGQ 250 260 270 280 290 300 QESIELGNLS ALRDWGHARE YVEAMWRILQ HDAPDDFVIA TGKQFSVREF CNLAFAEIGE 310 320 330 340 350 360 VLEWEGEGVE EVGKNKDGIV RVKVSPKYYR PTEVETLLGN PEKAKKTLGW EAKVTVPELV 370 KEMVASDIAL MKANPMA

References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed: 14624247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CAAC02000584 Genomic DNA. Translation: CAP38434.2.
3D structure databases
ProteinModelPortal	A8Y0L5.
ModBase	Search...
Protein-protein interaction databases
STRING	A8Y0L5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
WormBase	CBG21737; CBP34236; WBGene00040436.
Phylogenomic databases
HOGENOM	HBG755066.
Family and domain databases
InterPro	IPR001509. Epimerase_deHydtase. IPR006368. GDP_Man_deHydtase. IPR016040. NAD(P)-bd_dom. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
PANTHER	PTHR10366:SF32. GDP_mann_dehyd. 1 hit.
Pfam	PF01370. Epimerase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01472. Gmd. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GMD1_CAEBR

Accession

Primary (citable) accession number: A8Y0L5

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	September 22, 2009
Last modified:	December 14, 2011
This is version 26 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project