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A8XY95

- METH_CAEBR

UniProt

A8XY95 - METH_CAEBR

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Protein
Probable methionine synthase
Gene
metr-1, CBG20636
Organism
Caenorhabditis briggsae
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate By similarity.

Catalytic activityi

5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine.

Cofactori

Methylcobalamin (MeCBL) By similarity.
Binds 1 zinc ion per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi249 – 2491Zinc By similarity
Metal bindingi312 – 3121Zinc By similarity
Metal bindingi313 – 3131Zinc By similarity
Metal bindingi775 – 7751Cobalt (cobalamin axial ligand) By similarity
Binding sitei820 – 8201Cobalamin By similarity
Binding sitei977 – 9771S-adenosyl-L-methionine By similarity
Binding sitei1171 – 11711S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding sitei1175 – 11751Cobalamin; via carbonyl oxygen By similarity

GO - Molecular functioni

  1. S-adenosylmethionine-homocysteine S-methyltransferase activity Source: InterPro
  2. cobalamin binding Source: UniProtKB-KW
  3. methionine synthase activity Source: UniProtKB-EC
  4. zinc ion binding Source: InterPro

GO - Biological processi

  1. pteridine-containing compound metabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Cobalamin, Cobalt, Metal-binding, S-adenosyl-L-methionine, Zinc

Enzyme and pathway databases

UniPathwayiUPA00051; UER00081.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable methionine synthase (EC:2.1.1.13)
Alternative name(s):
5-methyltetrahydrofolate--homocysteine methyltransferase
Vitamin-B12 dependent methionine synthase
Short name:
MS
Gene namesi
Name:metr-1
ORF Names:CBG20636
OrganismiCaenorhabditis briggsae
Taxonomic identifieri6238 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000008549: Chromosome II, UP000008549: Unassembled WGS sequence

Organism-specific databases

WormBaseiCBG20636; CBP26766; WBGene00039583; Cbr-metr-1.

Subcellular locationi

GO - Cellular componenti

  1. intracellular Source: InterPro
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12731273Probable methionine synthase
PRO_0000412913Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi6238.CBG20636.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini7 – 327321Hcy-binding
Add
BLAST
Domaini360 – 621262Pterin-binding
Add
BLAST
Domaini652 – 74998B12-binding N-terminal
Add
BLAST
Domaini762 – 897136B12-binding
Add
BLAST
Domaini927 – 1273347AdoMet activation
Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni850 – 8512Cobalamin-binding By similarity
Regioni1225 – 12262S-adenosyl-L-methionine binding By similarity

Domaini

Modular enzyme with four functionally distinct domains. The isolated Hcy-binding domain catalyzes methyl transfer from free methylcobalamin to homocysteine. The Hcy-binding domain in association with the pterin-binding domain catalyzes the methylation of cob(I)alamin by methyltetrahydrofolate and the methylation of homocysteine. The B12-binding domain binds the cofactor. The AdoMet activation domain binds S-adenosyl-L-methionine. Under aerobic conditions cob(I)alamin can be converted to inactive cob(II)alamin. Reductive methylation by S-adenosyl-L-methionine and flavodoxin regenerates methylcobalamin By similarity.

Sequence similaritiesi

Contains 1 B12-binding domain.
Contains 1 Hcy-binding domain.

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG1410.
HOGENOMiHOG000251409.
OrthoDBiEOG7TF786.

Family and domain databases

Gene3Di1.10.1240.10. 1 hit.
3.10.196.10. 1 hit.
3.20.20.20. 1 hit.
3.20.20.330. 1 hit.
3.40.50.280. 1 hit.
InterProiIPR003759. Cbl-bd_cap.
IPR006158. Cobalamin-bd.
IPR011005. Dihydropteroate_synth-like.
IPR011822. MetH.
IPR000489. Pterin-binding.
IPR003726. S_MeTrfase.
IPR004223. VitB12-dep_Met_synth_activ_dom.
[Graphical view]
PfamiPF02310. B12-binding. 1 hit.
PF02607. B12-binding_2. 1 hit.
PF02965. Met_synt_B12. 1 hit.
PF00809. Pterin_bind. 1 hit.
PF02574. S-methyl_trans. 1 hit.
[Graphical view]
PIRSFiPIRSF000381. MetH. 1 hit.
SMARTiSM01018. B12-binding_2. 1 hit.
[Graphical view]
SUPFAMiSSF47644. SSF47644. 1 hit.
SSF51717. SSF51717. 1 hit.
SSF52242. SSF52242. 1 hit.
SSF56507. SSF56507. 1 hit.
SSF82282. SSF82282. 1 hit.
TIGRFAMsiTIGR02082. metH. 1 hit.
PROSITEiPS50974. ADOMET_ACTIVATION. 1 hit.
PS51332. B12_BINDING. 1 hit.
PS51337. B12_BINDING_NTER. 1 hit.
PS50970. HCY. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8XY95-1 [UniParc]FASTAAdd to Basket

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MTRSSLFKEL ADIAKERIMI IDGAMGTMIQ REYMEEHDFR GEILKDHDKP     50
LKGNNDLLSI TRPDIIYKIH KLYLEAGADF IETNTFSGTT IAQADYHCEH 100
LVHEINYQSA LVARRACDDV GAATGIRRYV CGAIGPTNRT LSISPSVEKP 150
DFRNVTFQEL VKAYGDQARS LIQGGVDVLL VETVFDSANA KAALFAIRTL 200
FEDEGVPEIP VFLSGTIVDM SGRTLSGQTG EAFLVSTKQG KPIAVGLNCA 250
LGAKDMRQFV QNMSLWSDTL ILCYPNAGLP NALGGYDETP EEMAEVLREF 300
AQDGLVNIIG GCCGTTPDHI NAMYKAVQGI SPRVPPADPH AGKMLLSGLE 350
PSIVGPETNF VNIGERCNVA GSRRFCNLIK NENYDTAIDV ARVQVDSGAQ 400
ILDVNMDDGL LDGPYAMSKF LRLISSEPDV AKIPVCIDSS DFDVIIAGLE 450
STQGKCVVNS ISLKEGEEKF KERARIVKRY GAAVVVMAFD EEGQAAETER 500
KFEICERSYR ILTEEVGFNP NDIIFDANIL TIATGMDEHA NYGMYFIEAT 550
RMIRENLPGA HVSGGVSNIS FSFRGMEAIR EAMHSVFLFY AIKAGMDMGI 600
VNAGALPVYE DIDKPLLQLL EDLLFNRDPE ATEKLLVAAQ EMKKDGKKAD 650
TKTDEWRNTS VEERLKFALV KGIDQFVVAD TEEARQNTEK YPRPLNVIER 700
PLMDGMAVVG ELFGAGKMFL PQVIKSARVM KKAVAHLLPF MDAERQANIE 750
KMGLDEDESP YQGTVVIATV KGDVHDIGKN IVAVVLGCNN FKVVDLGVMT 800
PCENIIKAAI EEKADFIGLS GLITPSLDEM VHVAKEMNRV GLKIPLLIGG 850
ATTSKTHTAV KIAPRYPHPV VHCLDASKSV VVCSSLSDMT VRDAFLQDLN 900
EDYEDVRTKM CLVSYLNHFF ITEHYESLKD RRFVALGKTR EKKFNIDWNK 950
FSPVKPSFIG RREFQNFDFK ELIPYIDWKP FFDVWQLRGK YPNRSYPKIF 1000
DDADVGGEAK RVFDDAQTWL KKLIDEKVLT ANAVVSFLPA ASEGDDIHVY 1050
DPETGNKLDT FYGLRQQSGR EHDQSHFCLS DFIRPLKIGV PDDYLGLFAC 1100
TAGLGAEEYC KVLEENHDDY ASIMVKALAD RLAEAYAEYL HKEVRVNLWG 1150
YSTNEQLTET DLLSIKYEGI RPACGYPSQP DHTEKRTLWK LLEAEKNGIV 1200
LTEHLAMLPA ASVSGLYFAN PQSQYFAVGK IDEDQVAFIY VRSKNVTDYA 1250
ARKNVPKEEV ERWLSPIIGY ELD 1273
Length:1,273
Mass (Da):141,762
Last modified:December 16, 2008 - v2
Checksum:i7178233645CE06AE
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
HE600991 Genomic DNA. Translation: CAP37612.2.

Genome annotation databases

EnsemblMetazoaiCBG20636; CBG20636; CBG20636.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
HE600991 Genomic DNA. Translation: CAP37612.2 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 6238.CBG20636.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai CBG20636 ; CBG20636 ; CBG20636 .

Organism-specific databases

WormBasei CBG20636 ; CBP26766 ; WBGene00039583 ; Cbr-metr-1.

Phylogenomic databases

eggNOGi COG1410.
HOGENOMi HOG000251409.
OrthoDBi EOG7TF786.

Enzyme and pathway databases

UniPathwayi UPA00051 ; UER00081 .

Family and domain databases

Gene3Di 1.10.1240.10. 1 hit.
3.10.196.10. 1 hit.
3.20.20.20. 1 hit.
3.20.20.330. 1 hit.
3.40.50.280. 1 hit.
InterProi IPR003759. Cbl-bd_cap.
IPR006158. Cobalamin-bd.
IPR011005. Dihydropteroate_synth-like.
IPR011822. MetH.
IPR000489. Pterin-binding.
IPR003726. S_MeTrfase.
IPR004223. VitB12-dep_Met_synth_activ_dom.
[Graphical view ]
Pfami PF02310. B12-binding. 1 hit.
PF02607. B12-binding_2. 1 hit.
PF02965. Met_synt_B12. 1 hit.
PF00809. Pterin_bind. 1 hit.
PF02574. S-methyl_trans. 1 hit.
[Graphical view ]
PIRSFi PIRSF000381. MetH. 1 hit.
SMARTi SM01018. B12-binding_2. 1 hit.
[Graphical view ]
SUPFAMi SSF47644. SSF47644. 1 hit.
SSF51717. SSF51717. 1 hit.
SSF52242. SSF52242. 1 hit.
SSF56507. SSF56507. 1 hit.
SSF82282. SSF82282. 1 hit.
TIGRFAMsi TIGR02082. metH. 1 hit.
PROSITEi PS50974. ADOMET_ACTIVATION. 1 hit.
PS51332. B12_BINDING. 1 hit.
PS51337. B12_BINDING_NTER. 1 hit.
PS50970. HCY. 1 hit.
PS50972. PTERIN_BINDING. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AF16.

Entry informationi

Entry nameiMETH_CAEBR
AccessioniPrimary (citable) accession number: A8XY95
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 21, 2011
Last sequence update: December 16, 2008
Last modified: July 9, 2014
This is version 48 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Miscellaneous

L-homocysteine is bound via the zinc atom By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi