Probable methionine synthase - Caenorhabditis briggsae

Preferred order of sections

Names and origin

Protein names

Recommended name:
Probable methionine synthase
EC=2.1.1.13

Alternative name(s):
5-methyltetrahydrofolate--homocysteine methyltransferase
Vitamin-B12 dependent methionine synthase
Short name=MS

Gene names

Name:	nos-1
ORF Names:	CBG20636

Organism

Caenorhabditis briggsae [Reference proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	1273 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from methyl-cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate By similarity.
Catalytic activity	5-methyltetrahydrofolate + L-homocysteine = tetrahydrofolate + L-methionine.
Cofactor	Cobalamin By similarity. Binds 1 zinc ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetH route): step 1/1.
Domain	Modular enzyme with four functionally distinct domains. The isolated Hcy-binding domain catalyzes methyl transfer from free methylcobalamin to homocysteine. The Hcy-binding domain in association with the pterin-binding domain catalyzes the methylation of cob(I)alamin by methyltetrahydrofolate and the methylation of homocysteine. The B12-binding domain binds the cofactor. The AdoMet activation domain binds S-adenosyl-L-methionine. Under aerobic conditions cob(I)alamin can be converted to inactive cob(II)alamin. Reductive methylation by S-adenosyl-L-methionine and flavodoxin regenerates methylcobalamin By similarity.
Miscellaneous	L-homocysteine is bound via the zinc atom By similarity.
Sequence similarities	Belongs to the vitamin-B12 dependent methionine synthase family. Contains 1 AdoMet activation domain. Contains 1 B12-binding domain. Contains 1 B12-binding N-terminal domain. Contains 1 Hcy-binding domain. Contains 1 pterin-binding domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Cobalamin Cobalt Metal-binding S-adenosyl-L-methionine Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	pteridine-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular_component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular_function	cobalamin binding Inferred from electronic annotation. Source: UniProtKB-KW homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: InterPro methionine synthase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1273

1273

Probable methionine synthase

PRO_0000412913

Regions

Domain

7 – 327

321

Hcy-binding

Domain

360 – 621

262

Pterin-binding

Domain

652 – 749

98

B12-binding N-terminal

Domain

762 – 897

136

B12-binding

Domain

927 – 1273

347

AdoMet activation

Region

850 – 851

2

Cobalamin-binding By similarity

Region

1225 – 1226

2

S-adenosyl-L-methionine binding By similarity

Sites

Metal binding

249

1

Zinc By similarity

Metal binding

312

1

Zinc By similarity

Metal binding

313

1

Zinc By similarity

Metal binding

775

1

Cobalt (cobalamin axial ligand) By similarity

Binding site

820

1

Cobalamin By similarity

Binding site

977

1

S-adenosyl-L-methionine By similarity

Binding site

1171

1

S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Binding site

1175

1

Cobalamin; via carbonyl oxygen By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

A8XY95 [UniParc].

Last modified December 16, 2008. Version 2.
Checksum: 7178233645CE06AE
Show »

FASTA

1,273

141,762

        10         20         30         40         50         60 
MTRSSLFKEL ADIAKERIMI IDGAMGTMIQ REYMEEHDFR GEILKDHDKP LKGNNDLLSI 

        70         80         90        100        110        120 
TRPDIIYKIH KLYLEAGADF IETNTFSGTT IAQADYHCEH LVHEINYQSA LVARRACDDV 

       130        140        150        160        170        180 
GAATGIRRYV CGAIGPTNRT LSISPSVEKP DFRNVTFQEL VKAYGDQARS LIQGGVDVLL 

       190        200        210        220        230        240 
VETVFDSANA KAALFAIRTL FEDEGVPEIP VFLSGTIVDM SGRTLSGQTG EAFLVSTKQG 

       250        260        270        280        290        300 
KPIAVGLNCA LGAKDMRQFV QNMSLWSDTL ILCYPNAGLP NALGGYDETP EEMAEVLREF 

       310        320        330        340        350        360 
AQDGLVNIIG GCCGTTPDHI NAMYKAVQGI SPRVPPADPH AGKMLLSGLE PSIVGPETNF 

       370        380        390        400        410        420 
VNIGERCNVA GSRRFCNLIK NENYDTAIDV ARVQVDSGAQ ILDVNMDDGL LDGPYAMSKF 

       430        440        450        460        470        480 
LRLISSEPDV AKIPVCIDSS DFDVIIAGLE STQGKCVVNS ISLKEGEEKF KERARIVKRY 

       490        500        510        520        530        540 
GAAVVVMAFD EEGQAAETER KFEICERSYR ILTEEVGFNP NDIIFDANIL TIATGMDEHA 

       550        560        570        580        590        600 
NYGMYFIEAT RMIRENLPGA HVSGGVSNIS FSFRGMEAIR EAMHSVFLFY AIKAGMDMGI 

       610        620        630        640        650        660 
VNAGALPVYE DIDKPLLQLL EDLLFNRDPE ATEKLLVAAQ EMKKDGKKAD TKTDEWRNTS 

       670        680        690        700        710        720 
VEERLKFALV KGIDQFVVAD TEEARQNTEK YPRPLNVIER PLMDGMAVVG ELFGAGKMFL 

       730        740        750        760        770        780 
PQVIKSARVM KKAVAHLLPF MDAERQANIE KMGLDEDESP YQGTVVIATV KGDVHDIGKN 

       790        800        810        820        830        840 
IVAVVLGCNN FKVVDLGVMT PCENIIKAAI EEKADFIGLS GLITPSLDEM VHVAKEMNRV 

       850        860        870        880        890        900 
GLKIPLLIGG ATTSKTHTAV KIAPRYPHPV VHCLDASKSV VVCSSLSDMT VRDAFLQDLN 

       910        920        930        940        950        960 
EDYEDVRTKM CLVSYLNHFF ITEHYESLKD RRFVALGKTR EKKFNIDWNK FSPVKPSFIG 

       970        980        990       1000       1010       1020 
RREFQNFDFK ELIPYIDWKP FFDVWQLRGK YPNRSYPKIF DDADVGGEAK RVFDDAQTWL 

      1030       1040       1050       1060       1070       1080 
KKLIDEKVLT ANAVVSFLPA ASEGDDIHVY DPETGNKLDT FYGLRQQSGR EHDQSHFCLS 

      1090       1100       1110       1120       1130       1140 
DFIRPLKIGV PDDYLGLFAC TAGLGAEEYC KVLEENHDDY ASIMVKALAD RLAEAYAEYL 

      1150       1160       1170       1180       1190       1200 
HKEVRVNLWG YSTNEQLTET DLLSIKYEGI RPACGYPSQP DHTEKRTLWK LLEAEKNGIV 

      1210       1220       1230       1240       1250       1260 
LTEHLAMLPA ASVSGLYFAN PQSQYFAVGK IDEDQVAFIY VRSKNVTDYA ARKNVPKEEV 

      1270 
ERWLSPIIGY ELD

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References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics."
Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W.

Waterston R.H.
PLoS Biol. 1:166-192(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: AF16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HE600991 Genomic DNA. Translation: CAP37612.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	6238.CBG20636.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	CBG20636; CBG20636; CBG20636.
Organism-specific databases
WormBase	CBG20636; CBP26766; WBGene00039583.
Phylogenomic databases
eggNOG	COG1410.
HOGENOM	HOG000251409.
Enzyme and pathway databases
UniPathway	UPA00051; UER00081.
Family and domain databases
Gene3D	1.10.1240.10. 1 hit. 3.10.196.10. 1 hit. 3.20.20.20. 1 hit. 3.20.20.330. 1 hit. 3.40.50.280. 1 hit.
InterPro	IPR003759. Cbl-bd_cap. IPR006158. Cobalamin-bd. IPR011005. Dihydropteroate_synth-like. IPR011822. MetH. IPR000489. Pterin-binding. IPR003726. S_MeTrfase. IPR004223. VitB12-dep_Met_synth_activ_dom. [Graphical view]
PANTHER	PTHR21091:SF9. PTHR21091:SF9. 1 hit.
Pfam	PF02310. B12-binding. 1 hit. PF02607. B12-binding_2. 1 hit. PF02965. Met_synt_B12. 1 hit. PF00809. Pterin_bind. 1 hit. PF02574. S-methyl_trans. 1 hit. [Graphical view]
PIRSF	PIRSF000381. MetH. 1 hit.
SMART	SM01018. B12-binding_2. 1 hit. [Graphical view]
SUPFAM	SSF52242. Cbl-bd. 1 hit. SSF51717. DHP_synth_like. 1 hit. SSF56507. Met_synth_B12. 1 hit. SSF47644. Met_synth_Cbl-bd. 1 hit. SSF82282. S_methyl_trans. 1 hit.
TIGRFAMs	TIGR02082. metH. 1 hit.
PROSITE	PS50974. ADOMET_ACTIVATION. 1 hit. PS51332. B12_BINDING. 1 hit. PS51337. B12_BINDING_NTER. 1 hit. PS50970. HCY. 1 hit. PS50972. PTERIN_BINDING. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METH_CAEBR

Accession

Primary (citable) accession number: A8XY95

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 21, 2011
Last sequence update:	December 16, 2008
Last modified:	April 3, 2013
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents