A8XT16 (GSTO2_CAEBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glutathione transferase omega-2
EC=2.5.1.18

Alternative name(s):
Glutathione-dependent dehydroascorbate reductase
EC=1.8.5.1
Monomethylarsonic acid reductase
Short name=MMA(V) reductase
EC=1.20.4.2

Gene names

Name:	gsto-2
ORF Names:	CBG18105

Organism

Caenorhabditis briggsae [Reference proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	253 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exhibits glutathione-dependent thiol transferase activity. Has dehydroascorbate reductase activity and may contribute to the recycling of ascorbic acid. Participates in the biotransformation of inorganic arsenic and reduces monomethylarsonic acid (MMA) By similarity.
Catalytic activity	RX + glutathione = HX + R-S-glutathione. 2 glutathione + dehydroascorbate = glutathione disulfide + ascorbate. Methylarsonate + 2 glutathione = methylarsonite + glutathione disulfide + H2O.
Sequence similarities	Belongs to the GST superfamily. Omega family. Contains 1 GST C-terminal domain. Contains 1 GST N-terminal domain.

Ontologies

Keywords
Molecular function	Oxidoreductase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: InterPro
Molecular_function	glutathione dehydrogenase (ascorbate) activity Inferred from electronic annotation. Source: EC glutathione transferase activity Inferred from electronic annotation. Source: EC methylarsonate reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 253	253	Probable glutathione transferase omega-2		PRO_0000352791
Regions
	Domain	25 – 105	81	GST N-terminal
	Domain	110 – 238	129	GST C-terminal
	Region	89 – 90	2	Glutathione binding By similarity
Sites
	Active site	35	1	Nucleophile By similarity
	Binding site	62	1	Glutathione By similarity
	Binding site	75	1	Glutathione; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8XT16 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 51F08F8D028C57A5	FASTA	253	28,451
	10 20 30 40 50 60 MPVLAGINSK VLKNGDSEPS PPPAGIYRIY NMRFCPWAQR ALIYASVKNV PSEVINIHLK 70 80 90 100 110 120 EKPDWYFSKH YKGQVPALEL DEGKKHVIES AHIPEYLDDL FPESRILPSD PYEKVQQKLL 130 140 150 160 170 180 LERLAAVAPA FYAAAQAANN PEGRDEKYAA LVKAFEDAEK LLTGDFFSGK AKPGFADYLI 190 200 210 220 230 240 FPNYQRVFWL SHILPNSPFS SESFPGPNFP KLAKWYRTLD SIPEVAAASQ PTEMGVGFFN 250 DYLKGTPNYD YGL

References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HE600963 Genomic DNA. Translation: CAP35619.1.
RefSeq	XP_002642151.1. XM_002642105.1.
3D structure databases
ProteinModelPortal	A8XT16.
ModBase	Search...
Protein-protein interaction databases
STRING	6238.CBG18105.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	CBG18105; CBG18105; CBG18105.
GeneID	8584146.
KEGG	cbr:CBG18105.
Organism-specific databases
CTD	8584146.
WormBase	CBG18105; CBP10756; WBGene00037589; Cbr-gsto-2.
Phylogenomic databases
eggNOG	NOG288793.
HOGENOM	HOG000006560.
KO	K00799.
OMA	SVYINLT.
Family and domain databases
Gene3D	1.20.1050.10. 1 hit. 3.40.30.10. 1 hit.
InterPro	IPR010987. Glutathione-S-Trfase_C-like. IPR004045. Glutathione_S-Trfase_N. IPR017933. Glutathione_S_Trfase/Cl_chnl_C. IPR005442. GST_omega. IPR012336. Thioredoxin-like_fold. [Graphical view]
PRINTS	PR01625. GSTRNSFRASEO.
SUPFAM	SSF47616. GST_C_like. 1 hit. SSF52833. Thiordxn-like_fd. 1 hit.
PROSITE	PS50405. GST_CTER. 1 hit. PS50404. GST_NTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GSTO2_CAEBR

Accession

Primary (citable) accession number: A8XT16

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 14, 2008
Last sequence update:	January 15, 2008
Last modified:	April 3, 2013
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project