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A8XSC1

- NLK_CAEBR

UniProt

A8XSC1 - NLK_CAEBR

Protein

Serine/threonine kinase NLK

Gene

lit-1

Organism
Caenorhabditis briggsae
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi
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    • History
      Entry version 47 (01 Oct 2014)
      Sequence version 2 (16 Dec 2008)
      Previous versions | rss
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    Functioni

    Has a role in the Wnt signaling pathway controlling the asymmetry of cell divisions during embryogenesis. Operates in the AB and EMS cell lineages influencing cell specification. Required for body wall muscle development, endoderm development, pop-1 asymmetry and T-cell division asymmetry. Lit-1/wrm-1 complex regulates pop-1 localization and is required for pop-1/par-5 interaction By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.By similarity

    Cofactori

    Magnesium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei237 – 2371ATPPROSITE-ProRule annotation
    Active sitei391 – 3911Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi214 – 2229ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. MAP kinase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. multicellular organismal development Source: UniProtKB-KW
    2. Wnt signaling pathway Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Wnt signaling pathway

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serine/threonine kinase NLKBy similarity (EC:2.7.11.24)
    Alternative name(s):
    Loss of intestine protein 1
    Nemo-like kinaseBy similarity
    Gene namesi
    Name:lit-1
    Synonyms:nlkBy similarity
    ORF Names:CBG18286
    OrganismiCaenorhabditis briggsae
    Taxonomic identifieri6238 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000008549: Chromosome III, UP000008549: Unassembled WGS sequence

    Organism-specific databases

    WormBaseiCBG18286; CBP27835; WBGene00037733; Cbr-lit-1.

    Subcellular locationi

    Cytoplasmcell cortex By similarity. Nucleus By similarity
    Note: Located in the anterior cell cortex before and during asymmetric cell division. After division, located preferentially in the nucleus of the posterior daughter cell By similarity.By similarity

    GO - Cellular componenti

    1. cell cortex Source: UniProtKB-SubCell
    2. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 657657Serine/threonine kinase NLKPRO_0000372801Add
    BLAST

    Proteomic databases

    PRIDEiA8XSC1.

    Interactioni

    Subunit structurei

    Interacts with wrm-1 (via N-terminus); activates lit-1 kinase activity and the lit-1/wrm-1 dimer phosphorylates pop-1 which promotes pop-1 interaction with par-5 and translocation of pop-1 from the nucleus to the cytoplasm. Interacts with pop-1 (phosphorylated on 'Ser-118' and 'Ser-127'); dependent on lit-1/wrm-1 complex By similarity.By similarity

    Protein-protein interaction databases

    STRINGi6238.CBG18286.

    Structurei

    3D structure databases

    ProteinModelPortaliA8XSC1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini208 – 554347Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi53 – 209157Gln-richAdd
    BLAST
    Compositional biasi76 – 15681His-richAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000233024.
    OrthoDBiEOG72RMZ3.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 2 hits.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 2 hits.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8XSC1-1 [UniParc]FASTAAdd to Basket

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    MPSSTLLELA PNTHSKCKFE TRNRSSSSSS GCSSSSTELY DLAAAHAALI    50
    SRQQQILNQG IPIIPEHQLA AAAVAHHHHQ LHPSVQHQLV AGHHHHHLPQ 100
    VAHHPAILPR SDVIQQPSHF ALHQHLQNLV QQQQQQQALH HHQQLVGDMA 150
    LVSHTHPAAV GSTTCYEKNP QKQQQVPQIP TQPQVAHVSS NAILAAAQPF 200
    YQPPVQDSQP DRPIGYGAFG VVWSVTDPRS GKRVALKKMP NVFQNLASCK 250
    RVFREIKMLS SFRHDNVLSL LDILQPANPS FFQEFTSWHL TPSIHHQRLI 300
    SHIKPICLFF VLPLVSLLCA HMYVCMWWHG TALLEGRKET ITYVLTELMQ 350
    SDLHKIIVSP QTLTIDHVKV FVYQILRGLK YLHTANILHR DIKPGNLLVN 400
    SNCILKICDF GLARTWDSRD RLNMTHEVVT QYYRAPELLM GARRYTGAVD 450
    IWSVGCIFAE LLQRKILFQA AGPIEQLQMI IDLLGTPSQE AMKYACEGAK 500
    NHVLRAGPRA PNLQSLYRLS QQTTDDAVDL LVKLLKFNPD ERISVEEALS 550
    HPYLEEGRLR FHSCMCSCCY TKANVPSRIF SQELDPKHES PFDPKWEKDM 600
    SRLSMFELRE KMYQFVMDRP ALYGVALCIN PQSAAYKNFA SSSVAQASEL 650
    PPSPQAW 657
    Length:657
    Mass (Da):74,169
    Last modified:December 16, 2008 - v2
    Checksum:iA31A2CB3AD089A22
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HE600936 Genomic DNA. Translation: CAP35763.2.

    Genome annotation databases

    EnsemblMetazoaiCBG18286; CBG18286; CBG18286.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    HE600936 Genomic DNA. Translation: CAP35763.2 .

    3D structure databases

    ProteinModelPortali A8XSC1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 6238.CBG18286.

    Proteomic databases

    PRIDEi A8XSC1.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai CBG18286 ; CBG18286 ; CBG18286 .

    Organism-specific databases

    WormBasei CBG18286 ; CBP27835 ; WBGene00037733 ; Cbr-lit-1.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000233024.
    OrthoDBi EOG72RMZ3.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 2 hits.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 2 hits.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AF16.

    Entry informationi

    Entry nameiNLK_CAEBR
    AccessioniPrimary (citable) accession number: A8XSC1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 26, 2009
    Last sequence update: December 16, 2008
    Last modified: October 1, 2014
    This is version 47 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3