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A8XSC1

- NLK_CAEBR

UniProt

A8XSC1 - NLK_CAEBR

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Protein
Serine/threonine kinase NLK
Gene
lit-1, nlk, CBG18286
Organism
Caenorhabditis briggsae
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Has a role in the Wnt signaling pathway controlling the asymmetry of cell divisions during embryogenesis. Operates in the AB and EMS cell lineages influencing cell specification. Required for body wall muscle development, endoderm development, pop-1 asymmetry and T-cell division asymmetry. Lit-1/wrm-1 complex regulates pop-1 localization and is required for pop-1/par-5 interaction By similarity.By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.By similarity

Cofactori

Magnesium By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei237 – 2371ATP By similarity
Active sitei391 – 3911Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi214 – 2229ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. MAP kinase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. Wnt signaling pathway Source: UniProtKB-KW
  2. multicellular organismal development Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Wnt signaling pathway

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine kinase NLK (EC:2.7.11.24)
Alternative name(s):
Loss of intestine protein 1
Nemo-like kinase
Gene namesi
Name:lit-1
Synonyms:nlk
ORF Names:CBG18286
OrganismiCaenorhabditis briggsae
Taxonomic identifieri6238 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000008549: Chromosome III, UP000008549: Unassembled WGS sequence

Organism-specific databases

WormBaseiCBG18286; CBP27835; WBGene00037733; Cbr-lit-1.

Subcellular locationi

Cytoplasmcell cortex By similarity. Nucleus By similarity
Note: Located in the anterior cell cortex before and during asymmetric cell division. After division, located preferentially in the nucleus of the posterior daughter cell By similarity.By similarity

GO - Cellular componenti

  1. cell cortex Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 657657Serine/threonine kinase NLK
PRO_0000372801Add
BLAST

Proteomic databases

PRIDEiA8XSC1.

Interactioni

Subunit structurei

Interacts with wrm-1 (via N-terminus); activates lit-1 kinase activity and the lit-1/wrm-1 dimer phosphorylates pop-1 which promotes pop-1 interaction with par-5 and translocation of pop-1 from the nucleus to the cytoplasm. Interacts with pop-1 (phosphorylated on 'Ser-118' and 'Ser-127'); dependent on lit-1/wrm-1 complex By similarity.By similarity

Protein-protein interaction databases

STRINGi6238.CBG18286.

Structurei

3D structure databases

ProteinModelPortaliA8XSC1.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini208 – 554347Protein kinase
Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi53 – 209157Gln-rich
Add
BLAST
Compositional biasi76 – 15681His-rich
Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000233024.
OrthoDBiEOG72RMZ3.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 2 hits.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8XSC1-1 [UniParc]FASTAAdd to Basket

« Hide

MPSSTLLELA PNTHSKCKFE TRNRSSSSSS GCSSSSTELY DLAAAHAALI    50
SRQQQILNQG IPIIPEHQLA AAAVAHHHHQ LHPSVQHQLV AGHHHHHLPQ 100
VAHHPAILPR SDVIQQPSHF ALHQHLQNLV QQQQQQQALH HHQQLVGDMA 150
LVSHTHPAAV GSTTCYEKNP QKQQQVPQIP TQPQVAHVSS NAILAAAQPF 200
YQPPVQDSQP DRPIGYGAFG VVWSVTDPRS GKRVALKKMP NVFQNLASCK 250
RVFREIKMLS SFRHDNVLSL LDILQPANPS FFQEFTSWHL TPSIHHQRLI 300
SHIKPICLFF VLPLVSLLCA HMYVCMWWHG TALLEGRKET ITYVLTELMQ 350
SDLHKIIVSP QTLTIDHVKV FVYQILRGLK YLHTANILHR DIKPGNLLVN 400
SNCILKICDF GLARTWDSRD RLNMTHEVVT QYYRAPELLM GARRYTGAVD 450
IWSVGCIFAE LLQRKILFQA AGPIEQLQMI IDLLGTPSQE AMKYACEGAK 500
NHVLRAGPRA PNLQSLYRLS QQTTDDAVDL LVKLLKFNPD ERISVEEALS 550
HPYLEEGRLR FHSCMCSCCY TKANVPSRIF SQELDPKHES PFDPKWEKDM 600
SRLSMFELRE KMYQFVMDRP ALYGVALCIN PQSAAYKNFA SSSVAQASEL 650
PPSPQAW 657
Length:657
Mass (Da):74,169
Last modified:December 16, 2008 - v2
Checksum:iA31A2CB3AD089A22
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
HE600936 Genomic DNA. Translation: CAP35763.2.

Genome annotation databases

EnsemblMetazoaiCBG18286; CBG18286; CBG18286.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
HE600936 Genomic DNA. Translation: CAP35763.2 .

3D structure databases

ProteinModelPortali A8XSC1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 6238.CBG18286.

Proteomic databases

PRIDEi A8XSC1.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai CBG18286 ; CBG18286 ; CBG18286 .

Organism-specific databases

WormBasei CBG18286 ; CBP27835 ; WBGene00037733 ; Cbr-lit-1.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000233024.
OrthoDBi EOG72RMZ3.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 2 hits.
[Graphical view ]
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 2 hits.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AF16.

Entry informationi

Entry nameiNLK_CAEBR
AccessioniPrimary (citable) accession number: A8XSC1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: December 16, 2008
Last modified: April 16, 2014
This is version 46 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3