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A8XSC1 (NLK_CAEBR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 46. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Serine/threonine kinase NLK

EC=2.7.11.24
Alternative name(s):
Loss of intestine protein 1
Nemo-like kinase
Gene names
Name:lit-1
Synonyms:nlk
ORF Names:CBG18286
OrganismCaenorhabditis briggsae [Reference proteome]
Taxonomic identifier6238 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length657 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Has a role in the Wnt signaling pathway controlling the asymmetry of cell divisions during embryogenesis. Operates in the AB and EMS cell lineages influencing cell specification. Required for body wall muscle development, endoderm development, pop-1 asymmetry and T-cell division asymmetry. Lit-1/wrm-1 complex regulates pop-1 localization and is required for pop-1/par-5 interaction By similarity. UniProtKB Q9U9Y8

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. UniProtKB O54949

Cofactor

Magnesium By similarity. UniProtKB O54949

Subunit structure

Interacts with wrm-1 (via N-terminus); activates lit-1 kinase activity and the lit-1/wrm-1 dimer phosphorylates pop-1 which promotes pop-1 interaction with par-5 and translocation of pop-1 from the nucleus to the cytoplasm. Interacts with pop-1 (phosphorylated on 'Ser-118' and 'Ser-127'); dependent on lit-1/wrm-1 complex By similarity. UniProtKB Q9U9Y8

Subcellular location

Cytoplasmcell cortex By similarity. Nucleus By similarity. Note: Located in the anterior cell cortex before and during asymmetric cell division. After division, located preferentially in the nucleus of the posterior daughter cell By similarity. UniProtKB Q9U9Y8

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 657657Serine/threonine kinase NLK
PRO_0000372801

Regions

Domain208 – 554347Protein kinase
Nucleotide binding214 – 2229ATP By similarity
Compositional bias53 – 209157Gln-rich
Compositional bias76 – 15681His-rich

Sites

Active site3911Proton acceptor By similarity
Binding site2371ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
A8XSC1 [UniParc].

Last modified December 16, 2008. Version 2.
Checksum: A31A2CB3AD089A22

FASTA65774,169
        10         20         30         40         50         60 
MPSSTLLELA PNTHSKCKFE TRNRSSSSSS GCSSSSTELY DLAAAHAALI SRQQQILNQG 

        70         80         90        100        110        120 
IPIIPEHQLA AAAVAHHHHQ LHPSVQHQLV AGHHHHHLPQ VAHHPAILPR SDVIQQPSHF 

       130        140        150        160        170        180 
ALHQHLQNLV QQQQQQQALH HHQQLVGDMA LVSHTHPAAV GSTTCYEKNP QKQQQVPQIP 

       190        200        210        220        230        240 
TQPQVAHVSS NAILAAAQPF YQPPVQDSQP DRPIGYGAFG VVWSVTDPRS GKRVALKKMP 

       250        260        270        280        290        300 
NVFQNLASCK RVFREIKMLS SFRHDNVLSL LDILQPANPS FFQEFTSWHL TPSIHHQRLI 

       310        320        330        340        350        360 
SHIKPICLFF VLPLVSLLCA HMYVCMWWHG TALLEGRKET ITYVLTELMQ SDLHKIIVSP 

       370        380        390        400        410        420 
QTLTIDHVKV FVYQILRGLK YLHTANILHR DIKPGNLLVN SNCILKICDF GLARTWDSRD 

       430        440        450        460        470        480 
RLNMTHEVVT QYYRAPELLM GARRYTGAVD IWSVGCIFAE LLQRKILFQA AGPIEQLQMI 

       490        500        510        520        530        540 
IDLLGTPSQE AMKYACEGAK NHVLRAGPRA PNLQSLYRLS QQTTDDAVDL LVKLLKFNPD 

       550        560        570        580        590        600 
ERISVEEALS HPYLEEGRLR FHSCMCSCCY TKANVPSRIF SQELDPKHES PFDPKWEKDM 

       610        620        630        640        650 
SRLSMFELRE KMYQFVMDRP ALYGVALCIN PQSAAYKNFA SSSVAQASEL PPSPQAW 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
HE600936 Genomic DNA. Translation: CAP35763.2.

3D structure databases

ProteinModelPortalA8XSC1.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6238.CBG18286.

Proteomic databases

PRIDEA8XSC1.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaCBG18286; CBG18286; CBG18286.

Organism-specific databases

WormBaseCBG18286; CBP27835; WBGene00037733; Cbr-lit-1.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000233024.
OrthoDBEOG72RMZ3.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00069. Pkinase. 2 hits.
[Graphical view]
SMARTSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 2 hits.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameNLK_CAEBR
AccessionPrimary (citable) accession number: A8XSC1
Entry history
Integrated into UniProtKB/Swiss-Prot: May 26, 2009
Last sequence update: December 16, 2008
Last modified: April 16, 2014
This is version 46 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families