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Protein

S-methyl-5'-thioadenosine phosphorylase

Gene

CBG12968

Organism
Caenorhabditis briggsae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation

Catalytic activityi

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei10 – 101PhosphateUniRule annotation
Sitei170 – 1701Important for substrate specificityUniRule annotation
Binding sitei188 – 1881Substrate; via amide nitrogenUniRule annotation
Binding sitei189 – 1891PhosphateUniRule annotation
Sitei224 – 2241Important for substrate specificityUniRule annotation

GO - Molecular functioni

  1. phosphorylase activity Source: InterPro
  2. S-methyl-5-thioadenosine phosphorylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. L-methionine biosynthetic process from methylthioadenosine Source: UniProtKB-HAMAP
  2. purine ribonucleoside salvage Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine salvage

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Names & Taxonomyi

Protein namesi
Recommended name:
S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)
Alternative name(s):
5'-methylthioadenosine phosphorylaseUniRule annotation
Short name:
MTA phosphorylaseUniRule annotation
Short name:
MTAPUniRule annotation
Short name:
MTAPaseUniRule annotation
Gene namesi
ORF Names:CBG12968
OrganismiCaenorhabditis briggsae
Taxonomic identifieri6238 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000008549: Chromosome II, UP000008549: Unassembled WGS sequence

Organism-specific databases

WormBaseiCBG12968; CBP22764; WBGene00033820.

Subcellular locationi

Cytoplasm UniRule annotation. Nucleus UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
  2. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285S-methyl-5'-thioadenosine phosphorylasePRO_0000415122Add
BLAST

Interactioni

Subunit structurei

Homotrimer.UniRule annotation

Protein-protein interaction databases

STRINGi6238.CBG12968.

Structurei

3D structure databases

ProteinModelPortaliA8XGS6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni52 – 532Phosphate bindingUniRule annotation
Regioni85 – 862Phosphate bindingUniRule annotation
Regioni212 – 2143Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228987.
InParanoidiA8XGS6.
KOiK00772.
OMAiMTNHTEA.
OrthoDBiEOG771270.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

A8XGS6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVKVGIIGGS GLEDPNILLN PTAISIDTPY GQPSDHLIQG TINGVECVLL
60 70 80 90 100
ARHGRKHDIM PGNVNYRANL WALYSLGVDV IIASTACGSL KEEVAPGHLL
110 120 130 140 150
FPDSVFDRTN SRKATFFDGT FSGAPGVSHI QAHPTYNEKL RQVLISTAEK
160 170 180 190 200
CKLVHHRTGF GVCIEGPRFS TKAESMVFKS WGASLVNMTM MPECILAKEL
210 220 230 240 250
GIPYATTALV TDYDCWKDED QVTAASVMKV FAANVEKAKT LFIEAVAEIG
260 270 280
KIDWSAEILQ TKTAARQSIM ISPDVEVEFL KVPKT
Length:285
Mass (Da):31,011
Last modified:January 15, 2008 - v1
Checksum:i2156DCAD3564F43F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE600938 Genomic DNA. Translation: CAP31850.1.
RefSeqiXP_002630529.1. XM_002630483.1.

Genome annotation databases

EnsemblMetazoaiCBG12968; CBG12968; CBG12968.
GeneIDi8572045.
KEGGicbr:CBG12968.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE600938 Genomic DNA. Translation: CAP31850.1.
RefSeqiXP_002630529.1. XM_002630483.1.

3D structure databases

ProteinModelPortaliA8XGS6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6238.CBG12968.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiCBG12968; CBG12968; CBG12968.
GeneIDi8572045.
KEGGicbr:CBG12968.

Organism-specific databases

CTDi8572045.
WormBaseiCBG12968; CBP22764; WBGene00033820.

Phylogenomic databases

eggNOGiCOG0005.
HOGENOMiHOG000228987.
InParanoidiA8XGS6.
KOiK00772.
OMAiMTNHTEA.
OrthoDBiEOG771270.

Enzyme and pathway databases

UniPathwayiUPA00904; UER00873.

Family and domain databases

Gene3Di3.40.50.1580. 1 hit.
HAMAPiMF_01963. MTAP.
InterProiIPR010044. MTAP.
IPR000845. Nucleoside_phosphorylase_d.
IPR001369. PNP/MTAP.
IPR018099. Purine_phosphorylase-2_CS.
[Graphical view]
PANTHERiPTHR11904. PTHR11904. 1 hit.
PfamiPF01048. PNP_UDP_1. 1 hit.
[Graphical view]
SUPFAMiSSF53167. SSF53167. 1 hit.
TIGRFAMsiTIGR01694. MTAP. 1 hit.
PROSITEiPS01240. PNP_MTAP_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: AF16.

Entry informationi

Entry nameiMTAP_CAEBR
AccessioniPrimary (citable) accession number: A8XGS6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 25, 2012
Last sequence update: January 15, 2008
Last modified: January 7, 2015
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.