A8XE00 (PP4C1_CAEBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Serine/threonine-protein phosphatase 4 catalytic subunit 1
Short name=PP4C-1
EC=3.1.3.16

Gene names

Name:	pph-4.1
ORF Names:	CBG11776

Organism

Caenorhabditis briggsae [Reference proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Protein phosphatase which is essential for embryogenesis. Is required for centrosome maturation and is involved in gametogenesis. Required for meiotic spindle formation during spermatogenesis and for chiasmata formation during oogenesis. However, is apparently dispensable for female meiotic divisions, which do not depend on centrosomes By similarity.
Catalytic activity	A phosphoprotein + H2O = a protein + phosphate.
Cofactor	Binds 1 iron ion per subunit By similarity. Binds 1 manganese ion per subunit By similarity.
Subunit structure	Serine/threonine-protein phosphatase 4 (PP4) occurs in different assemblies of the catalytic and one or more regulatory subunits By similarity.
Subcellular location	Cytoplasm By similarity. Cytoplasm › cytoskeleton › centrosome By similarity. Note: Localizes at centrosomes from prophase to telophase but dot during interphase. Locolaized to cytoplasm throughout the cell cycle By similarity.
Sequence similarities	Belongs to the PPP phosphatase family. PP-4 (PP-X) subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	Iron Manganese Metal-binding
Molecular function	Hydrolase Protein phosphatase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell microtubule organizing center Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoprotein phosphatase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 333	333	Serine/threonine-protein phosphatase 4 catalytic subunit 1		PRO_0000353209
Sites
	Active site	140	1	Proton donor By similarity
	Metal binding	79	1	Iron By similarity
	Metal binding	81	1	Iron By similarity
	Metal binding	107	1	Iron By similarity
	Metal binding	107	1	Manganese By similarity
	Metal binding	139	1	Manganese By similarity
	Metal binding	189	1	Manganese By similarity
	Metal binding	264	1	Manganese By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8XE00 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 90F18DEC1D925BDC	FASTA	333	37,469
	10 20 30 40 50 60 MALAVADTQN ETFARSESPT SGPSDQLSTH DLDRHIEKLM RCELIAEQDV KTLCAKAREI 70 80 90 100 110 120 LAEEGNVQVI DSPVTICGDI HGQFYDLMEL FRVGGPVPNT NYLFLGDFVD RGFYSVETFL 130 140 150 160 170 180 LLLALKARYP DRMMLIRGNH ESRQITQVYG FYDECMRKYG NASVWKHCTE VFDYLALAAV 190 200 210 220 230 240 IDGKVFCVHG GLSPSISTMD QIRVIDRKQE VPHDGPMCDL LWSDPEEGNV GWGLSPRGAG 250 260 270 280 290 300 YLFGADASKT FCEANSVDLI CRAHQLVMEG YKWHFNEKVL TVWSAPNYCY RCGNVAAILE 310 320 330 LDENLNREFT IFEAAPQENR GAPAKKPHAD YFL

References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HE601289 Genomic DNA. Translation: CAP30806.1.
RefSeq	XP_002641024.1. XM_002640978.1.
3D structure databases
ProteinModelPortal	A8XE00.
SMR	A8XE00. Positions 25-333.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	CBG11776; CBG11776; CBG11776.
GeneID	8583018.
KEGG	cbr:CBG11776.
Organism-specific databases
CTD	8583018.
WormBase	CBG11776; CBP02888; WBGene00032850; Cbr-pph-4.1.
Phylogenomic databases
HOGENOM	HOG000172696.
KO	K15423.
OMA	GFKWHFN.
Family and domain databases
InterPro	IPR004843. Metallo_PEstase_dom. IPR006186. Ser/Thr-sp_prot-phosphatase. [Graphical view]
Pfam	PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR00114. STPHPHTASE.
SMART	SM00156. PP2Ac. 1 hit. [Graphical view]
PROSITE	PS00125. SER_THR_PHOSPHATASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PP4C1_CAEBR

Accession

Primary (citable) accession number: A8XE00

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 4, 2008
Last sequence update:	January 15, 2008
Last modified:	March 6, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project