A8XDJ2 (OTUBL_CAEBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin thioesterase otubain-like
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme otubain-like
Ubiquitin-specific-processing protease otubain-like

Gene names

Name:	otub-1
ORF Names:	CBG11576

Organism

Caenorhabditis briggsae [Reference proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	288 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Hydrolase that can remove conjugated ubiquitin from proteins and plays an important regulatory role at the level of protein turnover by preventing degradation. Specifically cleaves 'Lys-48'-linked polyubiquitin By similarity. UniProtKB Q9XVR6
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C65 family. Contains 1 OTU domain.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW omega peptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 288	288	Ubiquitin thioesterase otubain-like		PRO_0000394766
Regions
	Domain	76 – 275	200	OTU
Sites
	Active site	84	1	By similarity UniProtKB Q96FW1
	Active site	87	1	Nucleophile By similarity UniProtKB Q96FW1
	Active site	268	1	By similarity UniProtKB Q96FW1

Sequences

	Sequence		Length	Mass (Da)
	A8XDJ2 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: C82E79D93EE5DA87	FASTA	288	32,949
	10 20 30 40 50 60 MVNDTSENQP TTAGIVTTTE DELILQDQQM KRIEDEQKAS PLVGEKMPCA TLVSLYDQET 70 80 90 100 110 120 APAFFEKANE LAKVYSHIRF IRGDGNCFIR AIQVGLVEIL LNDKERLVKF IASCKEWTER 130 140 150 160 170 180 LVKLGFPDWT CTDFCEFFIE FIEKVRDGIH QKEDVFRIFN DDNTANYLLM FFRLITSGYL 190 200 210 220 230 240 KEHAAEYEPF LDEGMSLAQY CETEIEAMWK ESDHLGIIAL VRALNIRIRI EYMDRNAAPN 250 260 270 280 GGTHHNLPDG HDNATFTPDI TLLYRPGHYD LIYKAPAETS KPALPPVA

References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HE600985 Genomic DNA. Translation: CAP30711.1.
RefSeq	XP_002637711.1. XM_002637665.1.
3D structure databases
ProteinModelPortal	A8XDJ2.
ModBase	Search...
Protein-protein interaction databases
STRING	6238.CBG11576.
Protein family/group databases
MEROPS	C65.A01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	CBG11576; CBG11576; CBG11576.
GeneID	8579707.
KEGG	cbr:CBG11576.
Organism-specific databases
CTD	8579707.
WormBase	CBG11576; CBP17228; WBGene00032679; Cbr-otub-1.
Phylogenomic databases
eggNOG	COG5539.
HOGENOM	HOG000019496.
KO	K09602.
OMA	VVYLRLI.
Family and domain databases
InterPro	IPR003323. OTU. IPR019400. Peptidase_C65_otubain. IPR016615. Ubiquitin_thioesterase_Otubain. [Graphical view]
Pfam	PF10275. Peptidase_C65. 1 hit. [Graphical view]
PIRSF	PIRSF013503. Ubiquitin_thioesterase_Otubain. 1 hit.
PROSITE	PS50802. OTU. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

OTUBL_CAEBR

Accession

Primary (citable) accession number: A8XDJ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 15, 2010
Last sequence update:	January 15, 2008
Last modified:	April 3, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project