Reviewed, UniProtKB/Swiss-Prot A8XA58 (CDC2_CAEBR)

Names and origin

Protein names

Recommended name:
    Cell division control protein 2 homolog
    EC=2.7.11.22
    EC=2.7.11.23

Gene names

Name:	cdk-1
ORF Names:	CBG10007

Organism

Caenorhabditis briggsae [Complete proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Plays a key role in the control of the eukaryotic cell cycle. It is required in higher cells for entry into S-phase and mitosis. p34 is a component of the kinase complex that phosphorylates the repetitive C-terminus of RNA polymerase II By similarity. UniProtKB P06493
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB P06493 ATP + [DNA-directed RNA polymerase] = ADP + [DNA-directed RNA polymerase] phosphate. UniProtKB P06493
Enzyme regulation	Phosphorylation both activates and inactivates the enzyme depending on the site of phosphorylation By similarity. UniProtKB P34556
Subunit structure	Forms a stable but non-covalent complex with a regulatory subunit and with a cyclin. Interacts with cks-1 By similarity. UniProtKB P06493 UniProtKB P34556
Subcellular location	Nucleus By similarity UniProtKB P39951.
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Mitosis
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell division Inferred from sequence or structural similarity. Source: UniProtKB mitosis Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of meiotic cell cycle Inferred from sequence or structural similarity. Source: UniProtKB protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA polymerase II carboxy-terminal domain kinase activity Inferred from electronic annotation. Source: EC cyclin-dependent protein kinase activity Inferred from electronic annotation. Source: EC protein kinase binding Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Cell division control protein 2 homolog		PRO_0000353199
Regions
	Domain	16 – 306	291	Protein kinase
	Nucleotide binding	22 – 30	9	ATP By similarity
Sites
	Active site	140	1	Proton acceptor By similarity
	Binding site	45	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8XA58-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 46D6B9B2C3BA3727	FASTA	326	37,875
	10 20 30 40 50 60 MTAIRKNDMN YTLDDFTKLE KIGEGTYGVV YKGRNRRTQA MVAMKKIRLE SEDEGVPSTA 70 80 90 100 110 120 VREISLLKEL QHPNVVGLEA VIMQENRLYL IFEFLSYDLK RYMDTLSKEE YLPSETLKSY 130 140 150 160 170 180 TFQILQAMCF CHQRRVIHRD LKPQNLLVDE KGAIKLADFG LARAIGIPIR VYTHEVVTLW 190 200 210 220 230 240 YRAPEILMGA QRYSMGVDMW SIGCIFAEMA TKKPLFQGDS EIDELFRIFR ILGTPTELEW 250 260 270 280 290 300 NGVESLPDYK ATFPKWRENF LRDKFYDKKS GNYLMDEDAF SLLEGLLIYD PALRISSKKA 310 320 LHHPYFNDID TSKLPAGNYR GELQLE

References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed: 14624247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CAAC02000489 Genomic DNA. Translation: CAP29526.1.
RefSeq	XP_002641677.1.
3D structure databases
SMR	A8XA58. Positions 12-315.
ModBase	Search...
Genome annotation databases
GeneID	8583671.
Organism-specific databases
WormBase	WBGene00031495. Cbr-cdk-1.
Phylogenomic databases
HOGENOM	HBG755340.
OMA	LPDYKAT.
PhylomeDB	A8XA58.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CDC2_CAEBR

Accession

Primary (citable) accession number: A8XA58

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 4, 2008
Last sequence update:	January 15, 2008
Last modified:	February 9, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project