Reviewed, UniProtKB/Swiss-Prot A8X775 (DLK1_CAEBR)

Names and origin

Protein names

Recommended name:
    Mitogen-activated protein kinase kinase kinase dlk-1
    EC=2.7.11.25
Alternative name(s):
    Death-associated protein kinase-like kinase
    DAP kinase-like kinase

Gene names

Name:	dlk-1
ORF Names:	CBG08707

Organism

Caenorhabditis briggsae [Complete proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	857 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of a MAP kinase pathway that functions presynaptically to regulate synaptic architecture and presynaptic differentiation. Phosphorylates and activates mkk-4 By similarity. UniProtKB O01700
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB O43283
Cofactor	Magnesium By similarity. UniProtKB O43283
Subcellular location	Cell junction › synapse By similarity. UniProtKB O01700
Post-translational modification	Ubiquitinated by rpm-1. Negatively regulated by ubiquitination by fsn-1 bound rpm-1, followed by degradation By similarity.
Sequence similarities	Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily. UniProtKB O01700 Contains 1 protein kinase domain.

Ontologies

Keywords
Cellular component	Cell junction Synapse
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Ubl conjugation
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW synapse Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW MAP kinase kinase kinase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 857	857	Mitogen-activated protein kinase kinase kinase dlk-1		PRO_0000353197
Regions
	Domain	62 – 304	243	Protein kinase
	Nucleotide binding	68 – 76	9	ATP By similarity UniProtKB O43283
Sites
	Active site	173	1	Proton acceptor By similarity UniProtKB O43283
	Binding site	89	1	ATP By similarity UniProtKB O43283

Sequences

	Sequence		Length	Mass (Da)
	A8X775-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: F3FF5FBB27CFC75E	FASTA	857	96,346
	10 20 30 40 50 60 MMAAAGNASK PSLNNFYAEG LGHLREGLFS CFRPVLGYFG GKPTQEIEKT EDEGWEIPFD 70 80 90 100 110 120 AISNLEWLGS GSQGAVFHGQ YENRTVAVKK VNQLKETEIK HLRHLRHKNI IEFLGVCSKS 130 140 150 160 170 180 PCYCIVMEYC PKGQLCTVLR QKNLITRQMF SDWVKEIADG MHYLHQNKVI HRDLKSPNIL 190 200 210 220 230 240 ISAEDSIKIC DFGTSHLQKK NDSTMMSFCG TVSWMAPEMI KKEPCNEKVD VYSFGVVLWE 250 260 270 280 290 300 MLTRETPYAN IAQMAIIFGV GTNILNLPMP EEAPRGLVLL IKQCLSQKGR NRPSFSHIRQ 310 320 330 340 350 360 HWEIFKPELF EMTEDEWQVA WDSYREFAKA IQYPSTVTKD HGGPKSAFAM EEEMQRKRHE 370 380 390 400 410 420 QLNHIKDIRH MYEAKLKRTN KMYDKLQGCF TELKLKEHEL AEWERNLAER EQMHVYNSPR 430 440 450 460 470 480 SMSAAPRFQL RGNCYPNEAY EEMSSDEDGQ CPPCRGSPYR NSNMSTSSGA QSSPFSRQSS 490 500 510 520 530 540 CRSSAGQQTR RSEGANAQKI SRNDLLRHSS SYWETIGNRG SPARGSGFSQ DSGVWSAGVS 550 560 570 580 590 600 SMAINGGVQG GVPVTYAQTI YRNGEGRWSD GRIASRRRVS SSANKNLPPV FFTRDSPSRV 610 620 630 640 650 660 PHGVVNHSAP RSSSKLNRSS YPSRNAPHQL EDGCCCNHGR VPRAKSVAVS MATRGRSPTP 670 680 690 700 710 720 YDNDASDAAE NPDIHYELQI PETTSYDEAL KSIGETDDVE MDAANGVNPI YSSPITTYNN 730 740 750 760 770 780 PCHVNYENVT EENANDIDLT SSMDSRRSRA DDADVESSED EGNGNNILNT SMESEELRYR 790 800 810 820 830 840 IDTSQSTMMS SLERSLEIGA TRSDGLSDNE RRVQAVKHSI KTHRRTSSNP QAIIHQRIEE 850 YSSSATEDSD DAGAVRI

References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed: 14624247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CAAC02000482 Genomic DNA. Translation: CAP28486.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GenomeReviews	Gene locus dlk-1 in contig CU538964_GR.
Phylogenomic databases
OMA	RRSEGAN.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR001245. Tyr_pkinase. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DLK1_CAEBR

Accession

Primary (citable) accession number: A8X775

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 4, 2008
Last sequence update:	January 15, 2008
Last modified:	November 3, 2009
This is version 16 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project