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A8WUG4 (KPC3_CAEBR) Reviewed, UniProtKB/Swiss-Prot

Last modified November 16, 2011. Version 38. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Protein kinase C-like 3
EC=2.7.11.13
Alternative name(s):
Atypical protein kinase C-3
Short name=aPKC3
Gene names
Name:pkc-3
ORF Names:CBG02381
OrganismCaenorhabditis briggsae
Taxonomic identifier6238 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length597 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Required for the normal progression of embryogenesis and viability of the organism. Plays an indispensable role in establishing embryonic polarity and in recruiting and maintaining par-6 to the periphery, through interaction with par-3. Required for epithelial cell polarity in the distal spermatheca. Phosphorylates serine residues of num-1 By similarity. UniProtKB Q19266

Catalytic activity

ATP + a protein = ADP + a phosphoprotein. UniProtKB Q19266

Cofactor

Magnesium By similarity. UniProtKB Q19266

Subunit structure

Interaction with par-3 required for the peripheral localization of par-6 and to form a par-3/par-6/pkc-3 complex, which is activated when cdc-42 interacts with par-6. Binds avidly to the phosphotyrosine interaction domain (PID) of a novel pkc-3 adapter protein num-1, which enables tethering and targeting of pkc-3 to the cell periphery By similarity. UniProtKB Q19266

Subcellular location

Cytoplasm By similarity. Cytoplasm › cytoskeleton By similarity. Note: Targeted and anchored at the apical surface (villi) of intestinal and pharyngeal cells, and in proximity with the cortical actin cytoskeleton that lies under the plasma membrane. Tightly bound to organelles/cytoskeleton in six of the seven developmental stages. Accumulation in cytoplasm is restricted to L1 larvae and adults. Co-localized with par-3 at the anterior cortex of the 1-cell embryo By similarity. UniProtKB Q19266

Sequence similarities

Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily.

Contains 1 AGC-kinase C-terminal domain.

Contains 1 OPR domain.

Contains 1 phorbol-ester/DAG-type zinc finger.

Contains 1 protein kinase domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 597597Protein kinase C-like 3
PRO_0000318077

Regions

Domain12 – 9584OPR
Domain253 – 522270Protein kinase
Domain524 – 59572AGC-kinase C-terminal
Zinc finger127 – 17751Phorbol-ester/DAG-type
Nucleotide binding259 – 2679ATP By similarity UniProtKB P41743

Sites

Active site3771Proton acceptor By similarity UniProtKB P41743
Binding site2821ATP By similarity UniProtKB P41743

Sequences

Sequence LengthMass (Da)Tools
A8WUG4 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: 998A72AF1F4F9C75

FASTA59767,842
        10         20         30         40         50         60 
MSSPTSVEED GDIKLKTRFH GQVVVLYARP PLILDDFFAL LRDACKQHAK QDITVKWIDE 

        70         80         90        100        110        120 
DGDPISIDSQ MELDEAVRCL NVSQEAELNI HVFVGKPELP GLPCQGEDKT VYRRGARRWK 

       130        140        150        160        170        180 
KIYLYNGHRF QGKRLNRRIQ CFICHDYIWG IGRQGFRCVD CRLCVHKKCH RHVRTHCGQT 

       190        200        210        220        230        240 
PQGPNVPVAP SSGVGSLRGG RLDTSSSTTR SGGGIDNGAF HEHEIESPGS AKDMSRSTNG 

       250        260        270        280        290        300 
NGASKWAVSL NDFRLLTVIG RGSYAKVVQA EHIATRQIYA IKIIKKEMFN EDEDIDWVQT 

       310        320        330        340        350        360 
EKSVFEAASN YPFLVGLHSC FQTESRLFFV IEFVPGGDLM FHMQQQRRLP EEHARFYSGE 

       370        380        390        400        410        420 
IILALHFLHS RGIIYRDLKL DNVLIDAEGH IKLTDYGMCK ENINAGDLTS TFCGTPNYIA 

       430        440        450        460        470        480 
PEILRGDEYG FSVDWWALGV LMFEMMAGRS PFDIVGMQNS EENTEDYLFQ IILERQIRIP 

       490        500        510        520        530        540 
RSLSVRASNI LKGFLNKDPS QRLGCKLDIN DGLNDMKEHD FFRGFIDWEA LEQKAVAPPY 

       550        560        570        580        590 
HPAVESDRDL THFDHQFTDE PPQLSPDNSA VIARIDQSEF DGFEYVNPLQ MSREDSV

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CAAC02000450 Genomic DNA. Translation: CAP24126.3.
RefSeqXP_002630700.1. XM_002630654.1.

3D structure databases

ProteinModelPortalA8WUG4.
SMRA8WUG4. Positions 248-589.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID8572216.
KEGGcbr:CBG02381.

Organism-specific databases

CTD8572216.
WormBaseCBG02381; CBP00699; WBGene00025443; Cbr-pkc-3.

Phylogenomic databases

HOGENOMHBG755340.
OMAGRQGFKC.
PhylomeDBA8WUG4.

Family and domain databases

InterProIPR000961. AGC-kinase_C.
IPR020454. DAG/PE-bd.
IPR011009. Kinase-like_dom.
IPR000270. OPR_PB1.
IPR012233. PKC_zeta.
IPR017892. Pkinase_C.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000719. Prot_kinase_cat_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR017442. Se/Thr_kinase-like_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR002290. Ser/Thr_kinase_dom.
[Graphical view]
KOK06069.
PfamPF00130. C1_1. 1 hit.
PF00564. PB1. 1 hit.
PF00069. Pkinase. 1 hit.
PF00433. Pkinase_C. 1 hit.
[Graphical view]
PIRSFPIRSF000554. PKC_zeta. 1 hit.
PRINTSPR00008. DAGPEDOMAIN.
SMARTSM00109. C1. 1 hit.
SM00666. PB1. 1 hit.
SM00133. S_TK_X. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMSSF56112. Kinase_like. 1 hit.
PROSITEPS51285. AGC_KINASE_CTER. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPC3_CAEBR
AccessionPrimary (citable) accession number: A8WUG4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 5, 2008
Last sequence update: January 15, 2008
Last modified: November 16, 2011
This is version 38 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

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