Reviewed, UniProtKB/Swiss-Prot A8WUG4 (KPC3_CAEBR)

Names and origin

Protein names

Recommended name:
    Protein kinase C-like 3
    EC=2.7.11.13
Alternative name(s):
    Atypical protein kinase C-3
      Short name=aPKC3

Gene names

Name:	pkc-3
ORF Names:	CBG02381

Organism

Caenorhabditis briggsae [Complete proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	597 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required for the normal progression of embryogenesis and viability of the organism. Plays an indispensable role in establishing embryonic polarity and in recruiting and maintaining par-6 to the periphery, through interaction with par-3. Required for epithelial cell polarity in the distal spermatheca. Phosphorylates serine residues of num-1 By similarity. UniProtKB Q19266
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. UniProtKB Q19266
Cofactor	Magnesium By similarity. UniProtKB Q19266
Subunit structure	Interaction with par-3 required for the peripheral localization of par-6 and to form a par-3/par-6/pkc-3 complex, which is activated when cdc-42 interacts with par-6. Binds avidly to the phosphotyrosine interaction domain (PID) of a novel pkc-3 adapter protein num-1, which enables tethering and targeting of pkc-3 to the cell periphery By similarity. UniProtKB Q19266
Subcellular location	Cytoplasm By similarity. Cytoplasm › cytoskeleton By similarity. Note: Targeted and anchored at the apical surface (villi) of intestinal and pharyngeal cells, and in proximity with the cortical actin cytoskeleton that lies under the plasma membrane. Tightly bound to organelles/cytoskeleton in six of the seven developmental stages. Accumulation in cytoplasm is restricted to L1 larvae and adults. Co-localized with par-3 at the anterior cortex of the 1-cell embryo By similarity. UniProtKB Q19266
Sequence similarities	Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. PKC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 OPR domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain.
Sequence caution	The sequence CAP24126.2 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Differentiation Fertilization Gastrulation Gonadal differentiation
Cellular component	Cytoplasm Cytoskeleton
Domain	Zinc-finger
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Zinc
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW establishment or maintenance of cell polarity Inferred from sequence or structural similarity. Source: UniProtKB gastrulation Inferred from electronic annotation. Source: UniProtKB-KW gonadal mesoderm development Inferred from electronic annotation. Source: UniProtKB-KW intracellular signaling cascade Inferred from electronic annotation. Source: InterPro protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro single fertilization Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cortical actin cytoskeleton Inferred from sequence or structural similarity. Source: UniProtKB plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein domain specific binding Inferred from sequence or structural similarity. Source: UniProtKB protein kinase C activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 597	597	Protein kinase C-like 3		PRO_0000318077
Regions
	Domain	12 – 95	84	OPR
	Domain	253 – 522	270	Protein kinase
	Domain	524 – 595	72	AGC-kinase C-terminal
	Zinc finger	127 – 177	51	Phorbol-ester/DAG-type
	Nucleotide binding	259 – 267	9	ATP By similarity UniProtKB P41743
Sites
	Active site	377	1	Proton acceptor By similarity UniProtKB P41743
	Binding site	282	1	ATP By similarity UniProtKB P41743

Sequences

	Sequence		Length	Mass (Da)
	A8WUG4-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 998A72AF1F4F9C75	FASTA	597	67,842
	10 20 30 40 50 60 MSSPTSVEED GDIKLKTRFH GQVVVLYARP PLILDDFFAL LRDACKQHAK QDITVKWIDE 70 80 90 100 110 120 DGDPISIDSQ MELDEAVRCL NVSQEAELNI HVFVGKPELP GLPCQGEDKT VYRRGARRWK 130 140 150 160 170 180 KIYLYNGHRF QGKRLNRRIQ CFICHDYIWG IGRQGFRCVD CRLCVHKKCH RHVRTHCGQT 190 200 210 220 230 240 PQGPNVPVAP SSGVGSLRGG RLDTSSSTTR SGGGIDNGAF HEHEIESPGS AKDMSRSTNG 250 260 270 280 290 300 NGASKWAVSL NDFRLLTVIG RGSYAKVVQA EHIATRQIYA IKIIKKEMFN EDEDIDWVQT 310 320 330 340 350 360 EKSVFEAASN YPFLVGLHSC FQTESRLFFV IEFVPGGDLM FHMQQQRRLP EEHARFYSGE 370 380 390 400 410 420 IILALHFLHS RGIIYRDLKL DNVLIDAEGH IKLTDYGMCK ENINAGDLTS TFCGTPNYIA 430 440 450 460 470 480 PEILRGDEYG FSVDWWALGV LMFEMMAGRS PFDIVGMQNS EENTEDYLFQ IILERQIRIP 490 500 510 520 530 540 RSLSVRASNI LKGFLNKDPS QRLGCKLDIN DGLNDMKEHD FFRGFIDWEA LEQKAVAPPY 550 560 570 580 590 HPAVESDRDL THFDHQFTDE PPQLSPDNSA VIARIDQSEF DGFEYVNPLQ MSREDSV

References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed: 14624247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CAAC02000450 Genomic DNA. Translation: CAP24126.2. Sequence problems.
RefSeq	XP_002630700.1.
3D structure databases
SMR	A8WUG4. Positions 9-94, 106-178, 248-589.
ModBase	Search...
Genome annotation databases
GeneID	8572216.
Organism-specific databases
WormBase	WBGene00025443. Cbr-pkc-3.
Phylogenomic databases
HOGENOM	HBG755340.
OMA	KGDIMIT.
PhylomeDB	A8WUG4.
Enzyme and pathway databases
BRENDA	2.7.11.13. 261794.
Family and domain databases
InterPro	IPR000961. AGC-kinase_C. IPR020454. DAG/PE_bd. IPR011009. Kinase-like_dom. IPR000270. OPR_PB1. IPR015745. PKC. IPR012233. PKC_zeta. IPR017892. Pkinase_C. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_prot_kinase-like_dom. IPR008271. Ser/Thr_prot_kinase_AS. IPR002290. Ser/Thr_prot_kinase_dom. [Graphical view]
PANTHER	PTHR22985:SF86. PKC. 1 hit.
Pfam	PF00130. C1_1. 1 hit. PF00564. PB1. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view]
PIRSF	PIRSF000554. PKC_zeta. 1 hit.
PRINTS	PR00008. DAGPEDOMAIN.
SMART	SM00109. C1. 1 hit. SM00666. PB1. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view]
PROSITE	PS51285. AGC_KINASE_CTER. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. PS00479. ZF_DAG_PE_1. 1 hit. PS50081. ZF_DAG_PE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPC3_CAEBR

Accession

Primary (citable) accession number: A8WUG4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	January 15, 2008
Last modified:	February 9, 2010
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project