Reviewed, UniProtKB/Swiss-Prot A8WTJ7 (HOT_CAEBR)

Names and origin

Protein names

Recommended name:
    Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial
      Short name=HOT
    EC=1.1.99.24

Gene names

ORF Names:

CBG02769

Organism

Caenorhabditis briggsae [Complete proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	465 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the cofactor-independent reversible oxidation of gamma-hydroxybutyrate (GHB) to succinic semialdehyde (SSA) coupled to reduction of 2-ketoglutarate (2-KG) to D-2-hydroxyglutarate (D-2-HG). L-3-hydroxybutyrate (L-3-OHB) is also a substrate for HOT when using 2-KG as hydrogen acceptor, resulting in the formation of D-2-HG By similarity.
Catalytic activity	(S)-3-hydroxybutanoate + 2-oxoglutarate = acetoacetate + (R)-2-hydroxyglutarate. 2-oxoglutaric acid + 4-hydroxybutanoic acid = (R)-2-hydroxyglutaric acid + succinic semialdehyde.
Subcellular location	MitochondrionBy similarity.
Sequence similarities	Belongs to the iron-containing alcohol dehydrogenase family. Hydroxyacid-oxoacid transhydrogenase subfamily.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	molecular hydrogen transport Inferred from sequence or structural similarity. Source: UniProtKB oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrion Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	hydroxyacid-oxoacid transhydrogenase activity Inferred from sequence or structural similarity. Source: UniProtKB metal ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 465		Probable hydroxyacid-oxoacid transhydrogenase, mitochondrial		PRO_0000324755

Sequences

	Sequence		Length	Mass (Da)
	A8WTJ7-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 3B9DA79103CD2DE6	FASTA	465	50,702
	10 20 30 40 50 60 MSASLARGML HKMGGSCCPH HAPTTNPFRM AKLHGNNKST DYAFEMVCST LRFGKGVTLE 70 80 90 100 110 120 IGYDVRNLGA KKTLLITDKN VQNTIAFKNA EQALKMVNIE YEIFDDVLIE PSEKSMQKAI 130 140 150 160 170 180 AFAKSKPFDS FIAVGGGSVI DTTKAAALYA SNPEADFLDF VGPPFGKSLQ PKNPMLPLIA 190 200 210 220 230 240 VPTTAGTGSE TTAAAIMDLP EHKCKTGIRL RCIKPYLAVV DPLNVMSMPR NVAIYSGFDV 250 260 270 280 290 300 LCHALESYTA LPYDQRSPRP ARPEVRPVYQ GSNPISDVWS REALRIIGQY FRRSVFDPSD 310 320 330 340 350 360 EEARTEMLKA SSFAGIGFGN AGVHLCHGLS YPISSQAKGC VAHDYPQDKN LIPHGLSVMT 370 380 390 400 410 420 TAVADFEFTT AACPDRHLVA AQTLGADIPN NADNEYISRT LCDQLRGFMK DFGVPNGLKG 430 440 450 460 MGFEYSDIDH LTEAASHSVP NIVISPKSTD RDIISKLYEK SLTVY

References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed: 14624247] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBLGenBankDDBJ	CAAC02000450 Genomic DNA. Translation: CAP23809.1.
3D structure databases
ModBase	Search...
Organism-specific databases
WormBase	WBGene00025759. CBG02769.
Family and domain databases
InterPro	IPR001670. Fe_AlcDHase. [Graphical view]
Pfam	PF00465. Fe-ADH. 1 hit. [Graphical view]
PROSITE	PS00913. ADH_IRON_1. 1 hit. PS00060. ADH_IRON_2. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

HOT_CAEBR

Accession

Primary (citable) accession number: A8WTJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	January 15, 2008
Last modified:	November 25, 2008
This is version 7 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Caenorhabditis annotation project