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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

CBG02883

Organism
Caenorhabditis briggsae
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathwayi: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei74S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Binding sitei152S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1
Active sitei155UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, RNA-binding, Transferase, tRNA-binding
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
ORF Names:CBG02883
OrganismiCaenorhabditis briggsae
Taxonomic identifieri6238 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000008549 Componentsi: Chromosome II, Unassembled WGS sequence

Organism-specific databases

WormBaseiCBG02883; CBP00870; WBGene00025851.

Subcellular locationi

  • Nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003705631 – 256tRNA (guanine-N(7)-)-methyltransferaseAdd BLAST256

Proteomic databases

PRIDEiA8WTA7.

Interactioni

Protein-protein interaction databases

STRINGi6238.CBG02883.

Structurei

3D structure databases

ProteinModelPortaliA8WTA7.
SMRiA8WTA7.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni97 – 98S-adenosyl-L-methionine bindingUniRule annotation2
Regioni132 – 133S-adenosyl-L-methionine bindingUniRule annotation2
Regioni230 – 232S-adenosyl-L-methionine bindingUniRule annotation3

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3115. Eukaryota.
COG0220. LUCA.
HOGENOMiHOG000260965.
InParanoidiA8WTA7.
KOiK03439.
OMAiPNFFYKG.
OrthoDBiEOG091G0IQ7.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PTHR23417:SF20. PTHR23417:SF20. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.

Sequencei

Sequence statusi: Complete.

A8WTA7-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVDPVNSEM ELDNKPTCET VPGLPQKKHY RQRAHSNPHS DHDIEYPLTP
60 70 80 90 100
DHMDWSKQYG DYAAGRQVEF ADIGCGYGGL LMRLSPMFPE TLMIGMEIRV
110 120 130 140 150
KVSDYVNEKI QALRKHHEGA GHYRNIAVLR SNAMKYMPNY FRKGQLSKMF
160 170 180 190 200
FLFPDPHFKN KKHKWRIITP TLVSEYAYVL REQGLIYTIT DVKDLHDWMV
210 220 230 240 250
KHLNEHPLFE RLSDDEMKAD PVVAMLYEST EEGQKVTRND GEKWPAVFRR

LPNPPL
Length:256
Mass (Da):29,917
Last modified:January 15, 2008 - v1
Checksum:i45BBC098C8F2C376
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE601438 Genomic DNA. Translation: CAP23718.1.
RefSeqiXP_002631108.1. XM_002631062.1.

Genome annotation databases

EnsemblMetazoaiCBG02883; CBG02883; WBGene00025851.
GeneIDi8572622.
KEGGicbr:CBG02883.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
HE601438 Genomic DNA. Translation: CAP23718.1.
RefSeqiXP_002631108.1. XM_002631062.1.

3D structure databases

ProteinModelPortaliA8WTA7.
SMRiA8WTA7.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi6238.CBG02883.

Proteomic databases

PRIDEiA8WTA7.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiCBG02883; CBG02883; WBGene00025851.
GeneIDi8572622.
KEGGicbr:CBG02883.

Organism-specific databases

CTDi8572622.
WormBaseiCBG02883; CBP00870; WBGene00025851.

Phylogenomic databases

eggNOGiKOG3115. Eukaryota.
COG0220. LUCA.
HOGENOMiHOG000260965.
InParanoidiA8WTA7.
KOiK03439.
OMAiPNFFYKG.
OrthoDBiEOG091G0IQ7.

Enzyme and pathway databases

UniPathwayiUPA00989.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_03055. tRNA_methyltr_TrmB_euk. 1 hit.
InterProiView protein in InterPro
IPR029063. SAM-dependent_MTases.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
PANTHERiPTHR23417. PTHR23417. 1 hit.
PTHR23417:SF20. PTHR23417:SF20. 1 hit.
PfamiView protein in Pfam
PF02390. Methyltransf_4. 1 hit.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiView protein in PROSITE
PS51625. SAM_MT_TRMB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTRMB_CAEBR
AccessioniPrimary (citable) accession number: A8WTA7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 15, 2008
Last modified: March 15, 2017
This is version 61 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.