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A8WTA7 (TRMB_CAEBR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 48. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
tRNA (guanine-N(7)-)-methyltransferase

EC=2.1.1.33
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase
Gene names
ORF Names:CBG02883
OrganismCaenorhabditis briggsae [Reference proteome]
Taxonomic identifier6238 [NCBI]
Taxonomic lineageEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis

Protein attributes

Sequence length256 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_03055

Catalytic activity

S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_03055

Pathway

tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_03055

Subcellular location

Nucleus By similarity HAMAP-Rule MF_03055.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentNucleus
   LigandRNA-binding
S-adenosyl-L-methionine
tRNA-binding
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentnucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiontRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tRNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 256256tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_03055
PRO_0000370563

Regions

Region97 – 982S-adenosyl-L-methionine binding By similarity
Region132 – 1332S-adenosyl-L-methionine binding By similarity
Region230 – 2323S-adenosyl-L-methionine binding By similarity

Sites

Active site1551 By similarity
Binding site741S-adenosyl-L-methionine; via carbonyl oxygen By similarity
Binding site1521S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

Sequence LengthMass (Da)Tools
A8WTA7 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: 45BBC098C8F2C376

FASTA25629,917
        10         20         30         40         50         60 
MDVDPVNSEM ELDNKPTCET VPGLPQKKHY RQRAHSNPHS DHDIEYPLTP DHMDWSKQYG 

        70         80         90        100        110        120 
DYAAGRQVEF ADIGCGYGGL LMRLSPMFPE TLMIGMEIRV KVSDYVNEKI QALRKHHEGA 

       130        140        150        160        170        180 
GHYRNIAVLR SNAMKYMPNY FRKGQLSKMF FLFPDPHFKN KKHKWRIITP TLVSEYAYVL 

       190        200        210        220        230        240 
REQGLIYTIT DVKDLHDWMV KHLNEHPLFE RLSDDEMKAD PVVAMLYEST EEGQKVTRND 

       250 
GEKWPAVFRR LPNPPL 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
HE601438 Genomic DNA. Translation: CAP23718.1.
RefSeqXP_002631108.1. XM_002631062.1.

3D structure databases

ProteinModelPortalA8WTA7.
SMRA8WTA7. Positions 46-256.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING6238.CBG02883.

Proteomic databases

PRIDEA8WTA7.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaCBG02883; CBG02883; CBG02883.
GeneID8572622.
KEGGcbr:CBG02883.

Organism-specific databases

CTD8572622.
WormBaseCBG02883; CBP00870; WBGene00025851.

Phylogenomic databases

eggNOGCOG0220.
HOGENOMHOG000260965.
KOK03439.
OMARAHSNPI.
OrthoDBEOG7PS1GC.

Enzyme and pathway databases

UniPathwayUPA00989.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
HAMAPMF_03055. tRNA_methyltr_TrmB_euk.
InterProIPR029063. SAM-dependent_MTases-like.
IPR025763. Trm8_euk.
IPR003358. tRNA_(Gua-N-7)_MeTrfase.
[Graphical view]
PfamPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00091. TIGR00091. 1 hit.
PROSITEPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTRMB_CAEBR
AccessionPrimary (citable) accession number: A8WTA7
Entry history
Integrated into UniProtKB/Swiss-Prot: May 5, 2009
Last sequence update: January 15, 2008
Last modified: June 11, 2014
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways