A8WTA7 (TRMB_CAEBR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

ORF Names:

CBG02883

Organism

Caenorhabditis briggsae [Reference proteome]

Taxonomic identifier

6238 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Protostomia › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity.
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA.
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
Subcellular location	Nucleus By similarity.
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Ligand	RNA-binding S-adenosyl-L-methionine tRNA-binding
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Cellular_component	nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	tRNA (guanine-N(7)-)-methyltransferase		PRO_0000370563
Regions
	Region	97 – 98	2	S-adenosyl-L-methionine binding By similarity
	Region	132 – 133	2	S-adenosyl-L-methionine binding By similarity
	Region	230 – 232	3	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	155	1	By similarity
	Binding site	74	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	152	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8WTA7 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 45BBC098C8F2C376	FASTA	256	29,917
	10 20 30 40 50 60 MDVDPVNSEM ELDNKPTCET VPGLPQKKHY RQRAHSNPHS DHDIEYPLTP DHMDWSKQYG 70 80 90 100 110 120 DYAAGRQVEF ADIGCGYGGL LMRLSPMFPE TLMIGMEIRV KVSDYVNEKI QALRKHHEGA 130 140 150 160 170 180 GHYRNIAVLR SNAMKYMPNY FRKGQLSKMF FLFPDPHFKN KKHKWRIITP TLVSEYAYVL 190 200 210 220 230 240 REQGLIYTIT DVKDLHDWMV KHLNEHPLFE RLSDDEMKAD PVVAMLYEST EEGQKVTRND 250 GEKWPAVFRR LPNPPL

References

[1]

"The genome sequence of Caenorhabditis briggsae: a platform for comparative genomics." Stein L.D., Bao Z., Blasiar D., Blumenthal T., Brent M.R., Chen N., Chinwalla A., Clarke L., Clee C., Coghlan A., Coulson A., D'Eustachio P., Fitch D.H.A., Fulton L.A., Fulton R.E., Griffiths-Jones S., Harris T.W., Hillier L.W. , Kamath R., Kuwabara P.E., Mardis E.R., Marra M.A., Miner T.L., Minx P., Mullikin J.C., Plumb R.W., Rogers J., Schein J.E., Sohrmann M., Spieth J., Stajich J.E., Wei C., Willey D., Wilson R.K., Durbin R.M., Waterston R.H. PLoS Biol. 1:166-192(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AF16.

Cross-references

Sequence databases
EMBL GenBank DDBJ	HE601438 Genomic DNA. Translation: CAP23718.1.
RefSeq	XP_002631108.1. XM_002631062.1.
3D structure databases
ProteinModelPortal	A8WTA7.
SMR	A8WTA7. Positions 46-256.
ModBase	Search...
Protein-protein interaction databases
STRING	6238.CBG02883.
Proteomic databases
PRIDE	A8WTA7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblMetazoa	CBG02883; CBG02883; CBG02883.
GeneID	8572622.
KEGG	cbr:CBG02883.
Organism-specific databases
CTD	8572622.
WormBase	CBG02883; CBP00870; WBGene00025851.
Phylogenomic databases
eggNOG	COG0220.
HOGENOM	HOG000260965.
KO	K03439.
OMA	RAHSNPI.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. IPR025763. tRNA_(Gua-N-7)_MeTrfase_euk. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_CAEBR

Accession

Primary (citable) accession number: A8WTA7

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 5, 2009
Last sequence update:	January 15, 2008
Last modified:	April 3, 2013
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project