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A8WIP6

- CDK20_DANRE

UniProt

A8WIP6 - CDK20_DANRE

Protein

Cyclin-dependent kinase 20

Gene

cdk20

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 58 (01 Oct 2014)
      Sequence version 1 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Involved in cell growth. Activates cdk2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160' By similarity. Required for high-level Shh responses in the developing neural tube. Together with tbc1d32, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing gli2 to be properly activated in response to SHH signaling.By similarity1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei33 – 331ATPPROSITE-ProRule annotation
    Active sitei127 – 1271Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. cilium morphogenesis Source: ZFIN
    4. multicellular organismal development Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein, Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Cell cycle, Cell division

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cyclin-dependent kinase 20 (EC:2.7.11.22)
    Alternative name(s):
    Cell cycle-related kinase
    Cell division protein kinase 20
    Gene namesi
    Name:cdk20
    Synonyms:ccrk
    ORF Names:si:dkeyp-77f7.1, zgc:101530
    OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)
    Taxonomic identifieri7955 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
    ProteomesiUP000000437: Chromosome 8

    Organism-specific databases

    ZFINiZDB-GENE-041212-84. cdk20.

    Subcellular locationi

    Nucleus By similarity. Cytoplasm By similarity. Cell projectioncilium By similarity

    GO - Cellular componenti

    1. cilium Source: UniProtKB-SubCell
    2. cytoplasm Source: UniProtKB-SubCell
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell projection, Cilium, Cytoplasm, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Fish lacking cdk20 are viable during embryogenesis and early larval stages, but exhibit curvature of the body axis and curled cilia in distal kidney tubules.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 344344Cyclin-dependent kinase 20PRO_0000393616Add
    BLAST

    Proteomic databases

    PRIDEiA8WIP6.

    Expressioni

    Gene expression databases

    BgeeiA8WIP6.

    Interactioni

    Subunit structurei

    Monomer. Interacts with tbc1d32.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliA8WIP6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini4 – 288285Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00740000115348.
    HOGENOMiHOG000233024.
    HOVERGENiHBG014652.
    InParanoidiQ5PRE2.
    KOiK08817.
    OMAiRPFIPEG.
    OrthoDBiEOG75J0NH.
    PhylomeDBiA8WIP6.
    TreeFamiTF327240.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    A8WIP6-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDQYSILGRI GEGAHGIVFK AKHIETGETV ALKKVALRRL EDGIPNQALR    50
    EIKALQEIED NQYVVKLKDV FPHGTGFVLV FEYMLSDLSE VIRNSQRPLT 100
    ASQVKSYMMM LLKGVAFCHE NSIMHRDLKP ANLLISSTGH LKIADFGLAR 150
    LFSNEGDRLY SHQVATRWYR APELLYGARK YDEGVDLWAV GCIFGELLNN 200
    SPLFPGENDI EQLCCVLRVL GTPNQKVWPE ITELPDYNKI TFKENPPIPL 250
    EEIVPDTSPQ AVDLLKKFLV YPSKQRISAR QALLHPYFFT DPLPAHHSEL 300
    PIPQRGGKHS RQRMQPPHEF TVDRPLHESV VDPSLIQKHA MSCS 344
    Length:344
    Mass (Da):39,025
    Last modified:January 15, 2008 - v1
    Checksum:iD4C820FAF9C7FB62
    GO

    Sequence cautioni

    The sequence AAH86697.1 differs from that shown. Reason: Frameshift at position 84.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571687 Genomic DNA. Translation: CAP19557.1.
    BC086697 mRNA. Translation: AAH86697.1. Frameshift.
    RefSeqiNP_001008655.1. NM_001008655.1.
    XP_005166745.1. XM_005166688.1.
    XP_005166746.1. XM_005166689.1.
    UniGeneiDr.37814.

    Genome annotation databases

    EnsembliENSDART00000140098; ENSDARP00000113146; ENSDARG00000003867.
    GeneIDi494112.
    KEGGidre:494112.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BX571687 Genomic DNA. Translation: CAP19557.1 .
    BC086697 mRNA. Translation: AAH86697.1 . Frameshift.
    RefSeqi NP_001008655.1. NM_001008655.1.
    XP_005166745.1. XM_005166688.1.
    XP_005166746.1. XM_005166689.1.
    UniGenei Dr.37814.

    3D structure databases

    ProteinModelPortali A8WIP6.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi A8WIP6.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSDART00000140098 ; ENSDARP00000113146 ; ENSDARG00000003867 .
    GeneIDi 494112.
    KEGGi dre:494112.

    Organism-specific databases

    CTDi 23552.
    ZFINi ZDB-GENE-041212-84. cdk20.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00740000115348.
    HOGENOMi HOG000233024.
    HOVERGENi HBG014652.
    InParanoidi Q5PRE2.
    KOi K08817.
    OMAi RPFIPEG.
    OrthoDBi EOG75J0NH.
    PhylomeDBi A8WIP6.
    TreeFami TF327240.

    Miscellaneous databases

    NextBioi 20865621.
    PROi A8WIP6.

    Gene expression databases

    Bgeei A8WIP6.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The zebrafish reference genome sequence and its relationship to the human genome."
      Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M., Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I., Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.
      , White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y., Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B., Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S., Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M., Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J., Clee C., Oliver K., Clark R., Riddle C., Eliott D., Threadgold G., Harden G., Ware D., Mortimer B., Kerry G., Heath P., Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S., Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J., Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J., Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D., McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S., Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E., Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A., Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P., Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J., Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E., Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C., Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C., Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M., Oberlander M., Rudolph-Geiger S., Teucke M., Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M., Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M., de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C., Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.
      Nature 496:498-503(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Tuebingen.
    2. NIH - Zebrafish Gene Collection (ZGC) project
      Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    3. "Broad-minded links cell cycle-related kinase to cilia assembly and hedgehog signal transduction."
      Ko H.W., Norman R.X., Tran J., Fuller K.P., Fukuda M., Eggenschwiler J.T.
      Dev. Cell 18:237-247(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.

    Entry informationi

    Entry nameiCDK20_DANRE
    AccessioniPrimary (citable) accession number: A8WIP6
    Secondary accession number(s): Q5PRE2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 20, 2010
    Last sequence update: January 15, 2008
    Last modified: October 1, 2014
    This is version 58 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3