Cyclin-dependent kinase 20 - Danio rerio (Zebrafish)

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A8WIP6 (CDK20_DANRE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 48. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Cyclin-dependent kinase 20
EC=2.7.11.22

Alternative name(s):
Cell cycle-related kinase
Cell division protein kinase 20

Gene names

Name:	cdk20
Synonyms:	ccrk
ORF Names:	si:dkeyp-77f7.1, zgc:101530

Organism

Danio rerio (Zebrafish) (Brachydanio rerio) [Reference proteome]

Taxonomic identifier

7955 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Cyprinidae › Danio

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Involved in cell growth. Activates cdk2, a kinase involved in the control of the cell cycle, by phosphorylating residue 'Thr-160' By similarity. Required for high-level Shh responses in the developing neural tube. Together with bromi, controls the structure of the primary cilium by coordinating assembly of the ciliary membrane and axoneme, allowing gli2 to be properly activated in response to SHH signaling. Ref.3
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Monomer By similarity. Interacts with BROMI By similarity.
Subcellular location	Nucleus By similarity. Cytoplasm By similarity. Cell projection › cilium By similarity.
Disruption phenotype	Fish lacking cdk20 are viable during embryogenesis and early larval stages, but exhibit curvature of the body axis and curled cilia in distal kidney tubules. Ref.3
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. CDC2/CDKX subfamily. Contains 1 protein kinase domain.
Sequence caution	The sequence AAH86697.1 differs from that shown. Reason: Frameshift at position 84.

Ontologies

Keywords
Biological process	Cell cycle Cell division
Cellular component	Cell projection Cilium Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Developmental protein Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cell division Inferred from electronic annotation. Source: UniProtKB-KW cilium morphogenesis Inferred from mutant phenotype Ref.3. Source: ZFIN multicellular organismal development Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cilium Inferred from electronic annotation. Source: UniProtKB-SubCell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cyclin-dependent protein serine/threonine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 344

344

Cyclin-dependent kinase 20

PRO_0000393616

Regions

Domain

4 – 288

285

Protein kinase

Nucleotide binding

10 – 18

ATP By similarity

Sites

Active site

127

Proton acceptor By similarity

Binding site

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

A8WIP6 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: D4C820FAF9C7FB62
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FASTA

344

39,025

        10         20         30         40         50         60 
MDQYSILGRI GEGAHGIVFK AKHIETGETV ALKKVALRRL EDGIPNQALR EIKALQEIED 

        70         80         90        100        110        120 
NQYVVKLKDV FPHGTGFVLV FEYMLSDLSE VIRNSQRPLT ASQVKSYMMM LLKGVAFCHE 

       130        140        150        160        170        180 
NSIMHRDLKP ANLLISSTGH LKIADFGLAR LFSNEGDRLY SHQVATRWYR APELLYGARK 

       190        200        210        220        230        240 
YDEGVDLWAV GCIFGELLNN SPLFPGENDI EQLCCVLRVL GTPNQKVWPE ITELPDYNKI 

       250        260        270        280        290        300 
TFKENPPIPL EEIVPDTSPQ AVDLLKKFLV YPSKQRISAR QALLHPYFFT DPLPAHHSEL 

       310        320        330        340 
PIPQRGGKHS RQRMQPPHEF TVDRPLHESV VDPSLIQKHA MSCS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	The Danio rerio sequencing project at the Sanger Institute Submitted (JAN-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Tuebingen.
[2]	NIH - Zebrafish Gene Collection (ZGC) project Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.
[3]	"Broad-minded links cell cycle-related kinase to cilia assembly and hedgehog signal transduction." Ko H.W., Norman R.X., Tran J., Fuller K.P., Fukuda M., Eggenschwiler J.T. Dev. Cell 18:237-247(2010) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, FUNCTION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BX571687 Genomic DNA. Translation: CAP19557.1. BC086697 mRNA. Translation: AAH86697.1. Frameshift.
IPI	IPI00484737.
RefSeq	NP_001008655.1. NM_001008655.1.
UniGene	Dr.37814.
3D structure databases
ProteinModelPortal	A8WIP6.
ModBase	Search...
Proteomic databases
PRIDE	A8WIP6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSDART00000021607; ENSDARP00000006661; ENSDARG00000003867. ENSDART00000140098; ENSDARP00000113146; ENSDARG00000003867.
GeneID	494112.
KEGG	dre:494112.
Organism-specific databases
CTD	23552.
ZFIN	ZDB-GENE-041212-84. cdk20.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00680000099989.
HOGENOM	HOG000233024.
HOVERGEN	HBG014652.
InParanoid	Q5PRE2.
KO	K08817.
OrthoDB	EOG4DBTDX.
Gene expression databases
Bgee	A8WIP6.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	20865621.

Entry information

Entry name

CDK20_DANRE

Accession

Primary (citable) accession number: A8WIP6
Secondary accession number(s): Q5PRE2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 20, 2010
Last sequence update:	January 15, 2008
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents