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A8W969 (AGB34_AGAAL) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 27. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Beta-agarase AgaB34

EC=3.2.1.81
Gene names
Name:agaB34
OrganismAgarivorans albus
Taxonomic identifier182262 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaAlteromonadalesAlteromonadaceaeAgarivorans

Protein attributes

Sequence length453 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product.

Subcellular location

Secreted By similarity UniProtKB P85974.

Sequence similarities

Belongs to the glycosyl hydrolase 16 family. UniProtKB P85974

Contains 1 ricin B-type lectin domain.

Ontologies

Keywords
   Cellular componentSecreted
   DomainSignal
   LigandLectin
   Molecular functionGlycosidase
Hydrolase
   PTMDisulfide bond
Gene Ontology (GO)
   Biological_processcarbohydrate catabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentextracellular region

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionbeta-agarase activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 Potential
Chain24 – 453430Beta-agarase AgaB34
PRO_0000347324

Regions

Domain313 – 453141Ricin B-type lectin

Sites

Active site1471Nucleophile By similarity UniProtKB P07883
Active site1521Proton donor By similarity UniProtKB P07883

Amino acid modifications

Disulfide bond327 ↔ 346 By similarity
Disulfide bond375 ↔ 394 By similarity
Disulfide bond423 ↔ 442 By similarity

Sequences

Sequence LengthMass (Da)Tools
A8W969 [UniParc].

Last modified January 15, 2008. Version 1.
Checksum: 51C5FA72C4FB1BEF

FASTA45350,998
        10         20         30         40         50         60 
MKGFTKHSIL MACSIGLAIN ATAADWDNIP IPAELDAGQS WELQQNYSDS FNYSGKNSTF 

        70         80         90        100        110        120 
TGKWKDSYFH SWTGPGLTHW SSDESWVGDG NLIISASRRQ GTNKVNAGVI TSKTKVKYPI 

       130        140        150        160        170        180 
FLEASIKVSN LELSSNFWLL SENDQREIDV LEVYGGARQD WYAKNMSTNF HVFFRNNDNS 

       190        200        210        220        230        240 
IKNDYNDQTH FTPTWGNYWR DGFHRFGVYW KSPTDVTFYI DGQKTTKGAW SQVVMKDKDY 

       250        260        270        280        290        300 
TGAILDKSRY NMDQEAFIII DTEDHSWRSE AGHIATDADL ADSDKNKMYV DWIRVYKPTG 

       310        320        330        340        350        360 
GSTTPPTGDI TPPSGYTNLQ LAHSNRCVDV INGALWNGST YQQYSCNTGN NNQRFKFTKI 

       370        380        390        400        410        420 
ANNQYSINAK VSQLCMELAS GSSANGAKVQ QWICNHANSN QTWSLEDKGS NTFEIRNKQS 

       430        440        450 
GKCLEVANSS NANGGQIRQW ACTGATNQRF KFL 

« Hide

References

[1]"Gene cloning, expression, and characterization of a beta-agarase, AgaB34, from Agarivorans albus YKW-34."
Fu X.T., Lin H., Pan C.-H., Kim S.M.
Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: YKW-34.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
EU200967 Genomic DNA. Translation: ABW77762.1.

3D structure databases

ProteinModelPortalA8W969.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyCBM13. Carbohydrate-Binding Module Family 13.
GH16. Glycoside Hydrolase Family 16.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D2.60.120.200. 1 hit.
InterProIPR008985. ConA-like_lec_gl_sf.
IPR013320. ConA-like_subgrp.
IPR000772. Ricin_B_lectin.
[Graphical view]
PfamPF00652. Ricin_B_lectin. 1 hit.
[Graphical view]
SMARTSM00458. RICIN. 1 hit.
[Graphical view]
SUPFAMSSF49899. SSF49899. 1 hit.
SSF50370. SSF50370. 1 hit.
PROSITEPS50231. RICIN_B_LECTIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameAGB34_AGAAL
AccessionPrimary (citable) accession number: A8W969
Entry history
Integrated into UniProtKB/Swiss-Prot: September 2, 2008
Last sequence update: January 15, 2008
Last modified: February 19, 2014
This is version 27 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries