Reviewed, UniProtKB/Swiss-Prot A8W969 (AGB34_AGAAL)

Names and origin

Protein names

Recommended name:
    Beta-agarase AgaB34
    EC=3.2.1.81

Gene names

Name:

agaB34

Organism

Agarivorans albus

Taxonomic identifier

182262 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Alteromonadales › Alteromonadaceae › Agarivorans

Protein attributes

Sequence length	453 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-beta-D-galactosidic linkages in agarose, giving the tetramer as the predominant product.
Subcellular location	Secreted By similarity. UniProtKB P85974
Sequence similarities	Belongs to the glycosyl hydrolase 16 family. UniProtKB P85974 Contains 1 ricin B-type lectin domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Ligand	Lectin
Molecular function	Glycosidase Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	carbohydrate catabolic process Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	extracellular region Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	beta-agarase activity Inferred from sequence or structural similarity. Source: UniProtKB sugar binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 23	23	Potential
	Chain	24 – 453	430	Beta-agarase AgaB34		PRO_0000347324
Regions
	Domain	313 – 453	141	Ricin B-type lectin
Sites
	Active site	147	1	Nucleophile By similarity UniProtKB P07883
	Active site	152	1	Proton donor By similarity UniProtKB P07883
Amino acid modifications
	Disulfide bond	327 ↔ 346		By similarity
	Disulfide bond	375 ↔ 394		By similarity
	Disulfide bond	423 ↔ 442		By similarity

Sequences

	Sequence		Length	Mass (Da)
	A8W969-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: 51C5FA72C4FB1BEF	FASTA	453	50,998
	10 20 30 40 50 60 MKGFTKHSIL MACSIGLAIN ATAADWDNIP IPAELDAGQS WELQQNYSDS FNYSGKNSTF 70 80 90 100 110 120 TGKWKDSYFH SWTGPGLTHW SSDESWVGDG NLIISASRRQ GTNKVNAGVI TSKTKVKYPI 130 140 150 160 170 180 FLEASIKVSN LELSSNFWLL SENDQREIDV LEVYGGARQD WYAKNMSTNF HVFFRNNDNS 190 200 210 220 230 240 IKNDYNDQTH FTPTWGNYWR DGFHRFGVYW KSPTDVTFYI DGQKTTKGAW SQVVMKDKDY 250 260 270 280 290 300 TGAILDKSRY NMDQEAFIII DTEDHSWRSE AGHIATDADL ADSDKNKMYV DWIRVYKPTG 310 320 330 340 350 360 GSTTPPTGDI TPPSGYTNLQ LAHSNRCVDV INGALWNGST YQQYSCNTGN NNQRFKFTKI 370 380 390 400 410 420 ANNQYSINAK VSQLCMELAS GSSANGAKVQ QWICNHANSN QTWSLEDKGS NTFEIRNKQS 430 440 450 GKCLEVANSS NANGGQIRQW ACTGATNQRF KFL

References

[1]	"Gene cloning, expression, and characterization of a beta-agarase, AgaB34, from Agarivorans albus YKW-34." Fu X.T., Lin H., Pan C.-H., Kim S.M. Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YKW-34.

Cross-references

Sequence databases
EMBLGenBankDDBJ	EU200967 Genomic DNA. Translation: ABW77762.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR013320. ConA-like_subgrp. IPR008263. Glycoside_hydrolase_16_AS. IPR000772. Ricin_B_lectin. [Graphical view]
Gene3D	G3DSA:2.60.120.200. ConA_like_subgrp. 1 hit.
Pfam	PF00652. Ricin_B_lectin. 1 hit. [Graphical view]
SMART	SM00458. RICIN. 1 hit. [Graphical view]
PROSITE	PS01034. GLYCOSYL_HYDROL_F16. False negative. PS50231. RICIN_B_LECTIN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AGB34_AGAAL

Accession

Primary (citable) accession number: A8W969

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 2, 2008
Last sequence update:	January 15, 2008
Last modified:	October 13, 2009
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)