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Protein

Collagen alpha-5(VI) chain

Gene

COL6A5

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Collagen VI acts as a cell-binding protein.By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cell adhesion

Enzyme and pathway databases

ReactomeiR-HSA-1442490. Collagen degradation.
R-HSA-1650814. Collagen biosynthesis and modifying enzymes.
R-HSA-186797. Signaling by PDGF.
R-HSA-2022090. Assembly of collagen fibrils and other multimeric structures.
R-HSA-216083. Integrin cell surface interactions.
R-HSA-3000178. ECM proteoglycans.
R-HSA-419037. NCAM1 interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Collagen alpha-5(VI) chain
Alternative name(s):
Collagen alpha-1(XXIX) chain
von Willebrand factor A domain-containing protein 4
Gene namesi
Name:COL6A5
Synonyms:COL29A1, VWA4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 3

Organism-specific databases

HGNCiHGNC:26674. COL6A5.

Subcellular locationi

GO - Cellular componenti

  • collagen trimer Source: UniProtKB-KW
  • extracellular region Source: MGI
  • proteinaceous extracellular matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Extracellular matrix, Secreted

Pathology & Biotechi

Involvement in diseasei

Patients affected by atopic dermatitis display an abnormal distribution of COL29A1 mRNA and protein in skin suggesting that COL29A1 may be involved in the pathogenesis of the disease.

Organism-specific databases

DisGeNETi256076.
OpenTargetsiENSG00000172752.
PharmGKBiPA165696956.

Chemistry databases

ChEMBLiCHEMBL2364188.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_500029447519 – 2615Collagen alpha-5(VI) chainAdd BLAST2597

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi201N-linked (GlcNAc...)Sequence analysis1
Glycosylationi260N-linked (GlcNAc...)Sequence analysis1
Glycosylationi835N-linked (GlcNAc...)Sequence analysis1
Glycosylationi2509N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Prolines at the third position of the tripeptide repeating unit (G-X-Y) are hydroxylated in some or all of the chains.By similarity

Keywords - PTMi

Glycoprotein, Hydroxylation

Proteomic databases

PaxDbiA8TX70.
PRIDEiA8TX70.

PTM databases

iPTMnetiA8TX70.
PhosphoSitePlusiA8TX70.

Expressioni

Tissue specificityi

Expressed in skin, followed by lung, small intestine, colon and testis. In skin, it is expressed in the epidermis with strongest staining in suprabasal viable layers. In ATOD patients, it is absent in the most differentiated upper spinous and granular layers (at protein level).1 Publication

Gene expression databases

BgeeiENSG00000172752.
ExpressionAtlasiA8TX70. baseline and differential.
GenevisibleiA8TX70. HS.

Organism-specific databases

HPAiHPA043138.

Interactioni

Subunit structurei

Trimers composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI).Curated

Protein-protein interaction databases

STRINGi9606.ENSP00000265379.

Structurei

3D structure databases

ProteinModelPortaliA8TX70.
SMRiA8TX70.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 209VWFA 1PROSITE-ProRule annotationAdd BLAST180
Domaini236 – 413VWFA 2PROSITE-ProRule annotationAdd BLAST178
Domaini442 – 612VWFA 3PROSITE-ProRule annotationAdd BLAST171
Domaini628 – 797VWFA 4PROSITE-ProRule annotationAdd BLAST170
Domaini814 – 987VWFA 5PROSITE-ProRule annotationAdd BLAST174
Domaini1005 – 1178VWFA 6PROSITE-ProRule annotationAdd BLAST174
Domaini1194 – 1376VWFA 7PROSITE-ProRule annotationAdd BLAST183
Domaini1395 – 1446Collagen-like 1Sequence analysisAdd BLAST52
Domaini1434 – 1490Collagen-like 2Sequence analysisAdd BLAST57
Domaini1464 – 1520Collagen-like 3Sequence analysisAdd BLAST57
Domaini1524 – 1580Collagen-like 4Sequence analysisAdd BLAST57
Domaini1579 – 1629Collagen-like 5Sequence analysisAdd BLAST51
Domaini1674 – 1729Collagen-like 6Sequence analysisAdd BLAST56
Domaini1758 – 1965VWFA 8PROSITE-ProRule annotationAdd BLAST208
Domaini1963 – 2154VWFA 9PROSITE-ProRule annotationAdd BLAST192
Domaini2291 – 2487VWFA 10PROSITE-ProRule annotationAdd BLAST197

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni19 – 1394Nonhelical regionAdd BLAST1376
Regioni1395 – 1728Triple-helical regionAdd BLAST334
Regioni1729 – 2615Nonhelical regionAdd BLAST887

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi1430 – 1432Cell attachment siteSequence analysis3

Sequence similaritiesi

Belongs to the type VI collagen family.Curated
Contains 6 collagen-like domains.Sequence analysis
Contains 10 VWFA domains.PROSITE-ProRule annotation

Keywords - Domaini

Collagen, Repeat, Signal

Phylogenomic databases

eggNOGiKOG3544. Eukaryota.
ENOG410XNMM. LUCA.
GeneTreeiENSGT00760000119000.
HOVERGENiHBG107743.
InParanoidiA8TX70.
KOiK06238.
OMAiKCFPNAC.
PhylomeDBiA8TX70.

Family and domain databases

Gene3Di3.40.50.410. 9 hits.
InterProiIPR008160. Collagen.
IPR002035. VWF_A.
[Graphical view]
PfamiPF01391. Collagen. 4 hits.
PF00092. VWA. 9 hits.
[Graphical view]
SMARTiSM00327. VWA. 9 hits.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 10 hits.
PROSITEiPS50234. VWFA. 9 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: A8TX70-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MKILLIIFVL IIWTETLADQ SPGPGPVYAD VVFLVDSSDH LGPKSFPFVK
60 70 80 90 100
TFINKMINSL PIEANKYRVA LAQYSDEFHS EFHLSTFKGR SPMLNHLKKN
110 120 130 140 150
FQFIGGSLQI GKALQEAHRT YFSAPINGRD RKQFPPILVV LASAESEDEV
160 170 180 190 200
EEASKALQKD GVKIISVGVQ KASEENLKAM ATSHFHFNLR TIRDLSTFSQ
210 220 230 240 250
NMTQIIKDVT KYKEGAVDAD MQVHFPISCQ KDSLADLVFL VDESLGTGGN
260 270 280 290 300
LRHLQTFLEN ITSSMDVKEN CMRLGLMSYS NSAKTISFLK SSTTQSEFQQ
310 320 330 340 350
QIKNLSIQVG KSNTGAAIDQ MRRDGFSESY GSRRAQGVPQ IAVLVTHRPS
360 370 380 390 400
DDEVHDAALN LRLEDVNVFA LSIQGANNTQ LEEIVSYPPE QTISTLKSYA
410 420 430 440 450
DLETYSTKFL KKLQNEIWSQ ISTYAEQRNL DKTGCVDTKE ADIHFLIDGS
460 470 480 490 500
SSIQEKQFEQ IKRFMLEVTE MFSIGPDKVR VGVVQYSDDT EVEFYITDYS
510 520 530 540 550
NDIDLRKAIF NIKQLTGGTY TGKALDYILQ IIKNGMKDRM SKVPCYLIVL
560 570 580 590 600
TDGMSTDRVV EPAKRLRAEQ ITVHAVGIGA ANKIELQEIA GKEERVSFGQ
610 620 630 640 650
NFDALKSIKN EVVREICAEK GCEDMKADIM FLVDSSWSIG NENFRKMKIF
660 670 680 690 700
MKNLLTKIQI GADKTQIGVV QFSDKTKEEF QLNRYFTQQE ISDAIDRMSL
710 720 730 740 750
INEGTLTGKA LNFVGQYFTH SKGARLGAKK FLILITDGVA QDDVRDPARI
760 770 780 790 800
LRGKDVTIFS VGVYNANRSQ LEEISGDSSL VFHVENFDHL KALERKLIFR
810 820 830 840 850
VCALHDCKRI TLLDVVFVLD HSGSIKKQYQ DHMINLTIHL VKKADVGRDR
860 870 880 890 900
VQFGALKYSD QPNILFYLNT YSNRSAIIEN LRKRRDTGGN TYTAKALKHA
910 920 930 940 950
NALFTEEHGS RIKQNVKQML IVITDGESHD HDQLNDTALE LRNKGITIFA
960 970 980 990 1000
VGVGKANQKE LEGMAGNKNN TIYVDNFDKL KDVFTLVQER MCTEAPEVCH
1010 1020 1030 1040 1050
LQEADVIFLC DGSDRVSNSD FVTMTTFLSD LIDNFDIQSQ RMKIGMAQFG
1060 1070 1080 1090 1100
SNYQSIIELK NSLTKTQWKT QIQNVSKSGG FPRIDFALKK VSNMFNLHAG
1110 1120 1130 1140 1150
GRRNAGVPQT LVVITSGDPR YDVADAVKTL KDLGICVLVL GIGDVYKEHL
1160 1170 1180 1190 1200
LPITGNSEKI ITFQDFDKLK NVDVKKRIIR EICQSCGKTN CFMDIVVGFD
1210 1220 1230 1240 1250
ISTHVQGQPL FQGHPQLESY LPGILEDISS IKGVSCGAGT EAQVSLAFKV
1260 1270 1280 1290 1300
NSDQGFPAKF QIYQKAVFDS LLQVNVSGPT HLNAQFLRSL WDTFKDKSAS
1310 1320 1330 1340 1350
RGQVLLIFSD GLQSESNIML ENQSDRLREA GLDALLVVSL NTTAHHEFSS
1360 1370 1380 1390 1400
FEFGKRFDYR THLTIGMREL GKKLSQYLGN IAERTCCCTF CKCPGIPGPH
1410 1420 1430 1440 1450
GTRGLQAMKG SQGLKGSRGH RGEDGNPGVR GDTGPQGDKG IAGCPGAWGQ
1460 1470 1480 1490 1500
KGLKGFSGPK GGHGDDGIDG LDGEEGCHGF PGIKGEKGDP GSQGSPGSRG
1510 1520 1530 1540 1550
APGQYGEKGF PGDPGNPGQN NNIKGQKGSK GEQGRQGRSG QKGVQGSPSS
1560 1570 1580 1590 1600
RGSRGREGQR GLRGVSGEPG NPGPTGTLGA EGLQGPQGSQ GNPGRKGEKG
1610 1620 1630 1640 1650
SQGQKGPQGS PGLMGAKGST GRPGLLGKKG EPGLPGDLGP VGQTGQRGRQ
1660 1670 1680 1690 1700
GDSGIPGYGQ MGRKGVKGPR GFPGDAGQKG DIGNPGIPGG PGPKGFRGLA
1710 1720 1730 1740 1750
LTVGLKGEEG SRGLPGPPGQ RGIKGMAGQP VYSQCDLIRF LREHSPCWKE
1760 1770 1780 1790 1800
KCPAYPTELV FALDNSYDVT EESFNKTRDI ITSIVNDLNI RENNCPVGAR
1810 1820 1830 1840 1850
VAMVSYNSGT SYLIRWSDYN RKKQLLQQLS QIKYQDTTEP RDVGNAMRFV
1860 1870 1880 1890 1900
TRNVFKRTYA GANVRRVAVF FSNGQTASRS SIITATMEFS ALDISPTVFA
1910 1920 1930 1940 1950
FDERVFLEAF GFDNTGTFQV IPVPPNGENQ TLERLRRCAL CYDKCFPNAC
1960 1970 1980 1990 2000
IREAFLPEDS YMDVVFLIDN SRNIAKDEFK AVKALVSSVI DNFNIASDPL
2010 2020 2030 2040 2050
ISDSGDRIAL LSYSPWESSR RKMGTVKTEF DFITYDNQLL MKNHIQTSFQ
2060 2070 2080 2090 2100
QLNGEATIGR ALLWTTENLF PETPYLRKHK VIFVVSAGEN YERKEFVKMM
2110 2120 2130 2140 2150
ALRAKCQGYV IFVISLGSTR KDDMEELASY PLDQHLIQLG RIHKPDLNYI
2160 2170 2180 2190 2200
AKFLKPFLYS VRRGFNQYPP PMLEDACRLI NLGGENIQND GFQFVTELQE
2210 2220 2230 2240 2250
DFLGGNGFIG QELNSGRESP FVKTEDNGSD YLVYLPSQMF EPQKLMINYE
2260 2270 2280 2290 2300
KDQKSAEIAS LTSGHENYGR KEEPDHTYEP GDVSLQEYYM DVAFLIDASQ
2310 2320 2330 2340 2350
RVGSDEFKEV KAFITSVLDY FHIAPTPLTS TLGDRVAVLS YSPPGYMPNT
2360 2370 2380 2390 2400
EECPVYLEFD LVTYNSIHQM KHHLQDSQQL NGDVFIGHAL QWTIDNVFVG
2410 2420 2430 2440 2450
TPNLRKNKVI FVISAGETNS LDKDVLRNVS LRAKCQGYSI FVFSFGPKHN
2460 2470 2480 2490 2500
DKELEELASH PLDHHLVQLG RTHKPDWNYI IKFVKPFVHL IRRAINKYPT
2510 2520 2530 2540 2550
EDMKATCVNM TSPNPENGGT ENTVLLLPGI YEIKTENGDL FDEFDSQAQH
2560 2570 2580 2590 2600
LLVLGNNHSS GSETATDLMQ KLYLLFSTEK LAMKDKEKAH LEEISALVVD
2610
KQQEKEDKEM EATDI
Length:2,615
Mass (Da):289,926
Last modified:January 15, 2008 - v1
Checksum:i745874018AC47EAF
GO
Isoform 2 (identifier: A8TX70-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     2526-2526: L → W
     2527-2615: Missing.

Note: No experimental confirmation available.
Show »
Length:2,526
Mass (Da):279,931
Checksum:i57BCAE5953E94AB6
GO

Sequence cautioni

The sequence BAC04092 differs from that shown. Reason: Frameshift at position 2591.Curated
The sequence BAC04092 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence BAC85681 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2482K → R in BAC04092 (PubMed:14702039).Curated1
Sequence conflicti2512S → P in BAC04092 (PubMed:14702039).Curated1
Sequence conflicti2560S → N in CAP20002 (PubMed:18276594).Curated1
Sequence conflicti2560S → N in CAP20003 (PubMed:18276594).Curated1
Sequence conflicti2560S → N in BAC04092 (PubMed:14702039).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_059234455E → K.1 PublicationCorresponds to variant rs1453241dbSNPEnsembl.1
Natural variantiVAR_059235641N → H.Corresponds to variant rs9882852dbSNPEnsembl.1
Natural variantiVAR_059236805H → R.Corresponds to variant rs16827168dbSNPEnsembl.1
Natural variantiVAR_059237982D → G.Corresponds to variant rs11917356dbSNPEnsembl.1
Natural variantiVAR_0592381114I → M.Corresponds to variant rs1353613dbSNPEnsembl.1
Natural variantiVAR_0592391280T → P.1 PublicationCorresponds to variant rs12488457dbSNPEnsembl.1
Natural variantiVAR_0592401477C → S.Corresponds to variant rs1497312dbSNPEnsembl.1
Natural variantiVAR_0592411589S → P.Corresponds to variant rs16827497dbSNPEnsembl.1
Natural variantiVAR_0611192175D → N.Corresponds to variant rs60021408dbSNPEnsembl.1
Natural variantiVAR_0436072188Q → R.2 PublicationsCorresponds to variant rs9883988dbSNPEnsembl.1
Natural variantiVAR_0436082205G → D.2 PublicationsCorresponds to variant rs819085dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0339122526L → W in isoform 2. 1 Publication1
Alternative sequenceiVSP_0339132527 – 2615Missing in isoform 2. 1 PublicationAdd BLAST89

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU085556 mRNA. Translation: ABW81241.1.
AM906078 mRNA. Translation: CAP19997.1.
AM906079 mRNA. Translation: CAP19998.1.
AM906080 mRNA. Translation: CAP19999.1.
AM906081 mRNA. Translation: CAP20000.1.
AM906082 mRNA. Translation: CAP20001.1.
AM906083 mRNA. Translation: CAP20002.1.
AM906084 mRNA. Translation: CAP20003.1.
AC093004 Genomic DNA. No translation available.
AC117398 Genomic DNA. No translation available.
AK093199 mRNA. Translation: BAC04092.1. Sequence problems.
AK123718 mRNA. Translation: BAC85681.1. Different initiation.
RefSeqiNP_001265227.1. NM_001278298.1.
NP_694996.5. NM_153264.6. [A8TX70-2]
UniGeneiHs.205403.

Genome annotation databases

EnsembliENST00000312481; ENSP00000309762; ENSG00000172752. [A8TX70-1]
GeneIDi256076.
KEGGihsa:256076.
UCSCiuc062ntw.1. human. [A8TX70-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EU085556 mRNA. Translation: ABW81241.1.
AM906078 mRNA. Translation: CAP19997.1.
AM906079 mRNA. Translation: CAP19998.1.
AM906080 mRNA. Translation: CAP19999.1.
AM906081 mRNA. Translation: CAP20000.1.
AM906082 mRNA. Translation: CAP20001.1.
AM906083 mRNA. Translation: CAP20002.1.
AM906084 mRNA. Translation: CAP20003.1.
AC093004 Genomic DNA. No translation available.
AC117398 Genomic DNA. No translation available.
AK093199 mRNA. Translation: BAC04092.1. Sequence problems.
AK123718 mRNA. Translation: BAC85681.1. Different initiation.
RefSeqiNP_001265227.1. NM_001278298.1.
NP_694996.5. NM_153264.6. [A8TX70-2]
UniGeneiHs.205403.

3D structure databases

ProteinModelPortaliA8TX70.
SMRiA8TX70.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9606.ENSP00000265379.

Chemistry databases

ChEMBLiCHEMBL2364188.

PTM databases

iPTMnetiA8TX70.
PhosphoSitePlusiA8TX70.

Proteomic databases

PaxDbiA8TX70.
PRIDEiA8TX70.

Protocols and materials databases

DNASUi256076.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000312481; ENSP00000309762; ENSG00000172752. [A8TX70-1]
GeneIDi256076.
KEGGihsa:256076.
UCSCiuc062ntw.1. human. [A8TX70-1]

Organism-specific databases

CTDi256076.
DisGeNETi256076.
GeneCardsiCOL6A5.
H-InvDBHIX0003678.
HGNCiHGNC:26674. COL6A5.
HPAiHPA043138.
MIMi611916. gene.
neXtProtiNX_A8TX70.
OpenTargetsiENSG00000172752.
PharmGKBiPA165696956.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG3544. Eukaryota.
ENOG410XNMM. LUCA.
GeneTreeiENSGT00760000119000.
HOVERGENiHBG107743.
InParanoidiA8TX70.
KOiK06238.
OMAiKCFPNAC.
PhylomeDBiA8TX70.

Enzyme and pathway databases

ReactomeiR-HSA-1442490. Collagen degradation.
R-HSA-1650814. Collagen biosynthesis and modifying enzymes.
R-HSA-186797. Signaling by PDGF.
R-HSA-2022090. Assembly of collagen fibrils and other multimeric structures.
R-HSA-216083. Integrin cell surface interactions.
R-HSA-3000178. ECM proteoglycans.
R-HSA-419037. NCAM1 interactions.

Miscellaneous databases

GenomeRNAii256076.
PROiA8TX70.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000172752.
ExpressionAtlasiA8TX70. baseline and differential.
GenevisibleiA8TX70. HS.

Family and domain databases

Gene3Di3.40.50.410. 9 hits.
InterProiIPR008160. Collagen.
IPR002035. VWF_A.
[Graphical view]
PfamiPF01391. Collagen. 4 hits.
PF00092. VWA. 9 hits.
[Graphical view]
SMARTiSM00327. VWA. 9 hits.
[Graphical view]
SUPFAMiSSF53300. SSF53300. 10 hits.
PROSITEiPS50234. VWFA. 9 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCO6A5_HUMAN
AccessioniPrimary (citable) accession number: A8TX70
Secondary accession number(s): A9J6L2
, A9J6L4, A9J6L6, A9J6L7, A9J6M0, A9J6M1, A9J6M2, B5MEA7, Q6ZW26, Q8NA36
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: January 15, 2008
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 3
    Human chromosome 3: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.