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Protein

Chitin elicitor receptor kinase 1

Gene

CERK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Lysin motif (LysM) receptor kinase that functions as a cell surface receptor in chitin elicitor (chitooligosaccharides) signaling leading to innate immunity toward both biotic and abiotic stresses (e.g. tolerance to salinity, heavy-metal stresses, and Botrytis cinerea infection). Recognizes microbe-derived N-acetylglucosamine (NAG)-containing ligands. Involved in the resistance to pathogenic fungi Alternaria brassicicola and Erysiphe cichoracearum, probably by sensing microbe-associated molecular patterns (MAMP) and pathogen-associated molecular patterns (PAMP). Plays an essential role in detecting peptidoglycans (e.g. PGNs) and restricting bacterial growth. Target of the bacterial type III effector E3-ligase protein hopAB2/avrPtoB of Pseudomonas syringae pv. tomato DC3000 that mediates ubiquitination and subsequent proteolysis, thus blocking all defense responses by suppressing PAMP-triggered immunity (PTI). Mediates chitin-induced phosphorylation of PBL27 (PubMed:24750441).9 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Enzyme regulationi

Activated by chitin-mediated homodimerization.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei349ATP1
Active sitei441Proton acceptor1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi328 – 336ATP9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chitin binding Source: UniProtKB
  • chitosan binding Source: UniProtKB
  • identical protein binding Source: IntAct
  • kinase activity Source: TAIR
  • protein homodimerization activity Source: UniProtKB
  • protein kinase activity Source: UniProtKB
  • protein self-association Source: UniProtKB
  • protein serine/threonine kinase activity Source: UniProtKB
  • transmembrane receptor protein kinase activity Source: TAIR
  • transmembrane receptor protein serine/threonine kinase activity Source: GO_Central

GO - Biological processi

  • cell surface pattern recognition receptor signaling pathway Source: UniProtKB
  • cellular response to chitin Source: UniProtKB
  • cellular response to molecule of bacterial origin Source: UniProtKB
  • defense response to bacterium Source: UniProtKB
  • defense response to fungus, incompatible interaction Source: TAIR
  • detection of molecule of fungal origin Source: TAIR
  • detection of peptidoglycan Source: TAIR
  • innate immune response Source: TAIR
  • intracellular signal transduction Source: TAIR
  • protein autophosphorylation Source: UniProtKB
  • protein phosphorylation Source: TAIR
  • response to chitin Source: TAIR

Keywordsi

Molecular functionKinase, Receptor, Serine/threonine-protein kinase, Transferase
Biological processPlant defense
LigandATP-binding, Chitin-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Chitin elicitor receptor kinase 1 (EC:2.7.11.1)
Short name:
AtCERK1
Alternative name(s):
LysM domain receptor-like kinase 1
Short name:
LysM RLK1
Short name:
LysM-containing receptor-like kinase 1
Gene namesi
Name:CERK1
Synonyms:LYK1, RLK1
Ordered Locus Names:At3g21630
ORF Names:MIL23.20
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

AraportiAT3G21630.
TAIRilocus:2089860. AT3G21630.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 232ExtracellularSequence analysisAdd BLAST209
Transmembranei233 – 253HelicalSequence analysisAdd BLAST21
Topological domaini254 – 617CytoplasmicSequence analysisAdd BLAST364

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Complete loss of response to the chitin elicitor, including loss of MAPK activation, generation of reactive oxygen species, and transcriptional activation. Impaired chitin-mediated resistance against biotic and abiotic stresses such as tolerance to salinity, heavy-metal stresses, and Botrytis cinerea infection. Reduced resistance to incompatible fungi such as Erysiphe cichoracearum and Alternaria brassicicola. Enhanced susceptibility to Pseudomonas syringae pv. tomato DC3000 associated with peptidoglycan insensitivity. Impaired chitin-induced phosphorylation of PBL27 (PubMed:24750441).6 Publications

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi138A → H: Slower chitin-mediated phosphorylation. 1 Publication1
Mutagenesisi441D → V: Increased affinitiy for PBL27. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Sequence analysisAdd BLAST23
ChainiPRO_000042082624 – 617Chitin elicitor receptor kinase 1Add BLAST594

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi25 ↔ 93Combined sources1 Publication
Disulfide bondi29 ↔ 155Combined sources1 Publication
Glycosylationi40N-linked (GlcNAc...) asparagineCombined sources1 Publication1
Glycosylationi52N-linked (GlcNAc...) asparagineCombined sources1 Publication1
Disulfide bondi91 ↔ 153Combined sources1 Publication
Glycosylationi102N-linked (GlcNAc...) asparagineCombined sources1 Publication1
Glycosylationi123N-linked (GlcNAc...) asparagineCombined sources1 Publication1
Glycosylationi152N-linked (GlcNAc...) asparagineCombined sources1 Publication1
Modified residuei266Phosphoserine1 Publication1
Modified residuei268Phosphoserine1 Publication1
Modified residuei270Phosphoserine1 Publication1
Modified residuei274Phosphoserine1 Publication1
Modified residuei390PhosphotyrosineBy similarity1
Modified residuei479PhosphothreonineBy similarity1
Modified residuei519Phosphothreonine1 Publication1

Post-translational modificationi

Autophosphorylated. Autophosphorylation is induced by chitin and derivatives.2 Publications
Ubiquitinated and targeted to the proteasome by hopAB2/avrPtoB of Pseudomonas syringae pv. tomato DC3000.1 Publication

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiA8R7E6.

PTM databases

iPTMnetiA8R7E6.

Expressioni

Tissue specificityi

Expressed ubiquitously, with lowest expression in pollen.1 Publication

Inductioni

Induced upon treatment with chitin oligosaccharide elicitor and flagellin (e.g. flg22).2 Publications

Gene expression databases

GenevisibleiA8R7E6. AT.

Interactioni

Subunit structurei

Forms homodimers and homooligomers. Homodimerization is required to trigger plant defenses. Binds to chitin, chitosan and chito-oligomer oligosaccharide elicitors. Interaction with chitin octamer (NAG8) promotes homodimerization while shorter chitin oligomers inhibit homodimerization. Interacts with Pseudomonas syringae hopAB2/avrPtoB. Interacts (preferentially when unphosphorylated) with PBL27 at the plasma membrane (PubMed:24750441, PubMed:27679653). Binds to IOS1 (PubMed:27317676).9 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-15672582,EBI-15672582

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • protein homodimerization activity Source: UniProtKB
  • protein self-association Source: UniProtKB

Protein-protein interaction databases

BioGridi7049. 66 interactors.
IntActiA8R7E6. 1 interactor.
STRINGi3702.AT3G21630.1.

Structurei

Secondary structure

1617
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi32 – 37Combined sources6
Helixi44 – 50Combined sources7
Beta strandi54 – 56Combined sources3
Helixi64 – 67Combined sources4
Beta strandi84 – 89Combined sources6
Beta strandi92 – 94Combined sources3
Turni95 – 97Combined sources3
Beta strandi98 – 106Combined sources9
Helixi113 – 118Combined sources6
Turni119 – 123Combined sources5
Helixi127 – 133Combined sources7
Helixi138 – 140Combined sources3
Beta strandi146 – 152Combined sources7
Turni158 – 160Combined sources3
Beta strandi167 – 171Combined sources5
Helixi178 – 185Combined sources8
Helixi189 – 195Combined sources7
Beta strandi205 – 211Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4EBYX-ray1.65A25-230[»]
4EBZX-ray1.79A25-230[»]
ProteinModelPortaliA8R7E6.
SMRiA8R7E6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini46 – 74LysM 1; degenerateAdd BLAST29
Domaini108 – 140LysM 2; degenerateAdd BLAST33
Domaini168 – 211LysM 3Add BLAST44
Domaini322 – 594Protein kinaseAdd BLAST273

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni109 – 115Chitin-binding7
Regioni137 – 143Chitin-binding7

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410II96. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116550.
InParanoidiA8R7E6.
KOiK13429.
OMAiSYPANNI.
OrthoDBiEOG093604SX.
PhylomeDBiA8R7E6.

Family and domain databases

CDDicd00118. LysM. 1 hit.
Gene3Di1.20.5.100. 1 hit.
3.10.350.10. 2 hits.
InterProiView protein in InterPro
IPR021157. Cyt_c1_TM_anchor_C.
IPR011009. Kinase-like_dom_sf.
IPR018392. LysM_dom.
IPR036779. LysM_dom_sf.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
PfamiView protein in Pfam
PF01476. LysM. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
SMARTiView protein in SMART
SM00220. S_TKc. 1 hit.
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiView protein in PROSITE
PS51782. LYSM. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

A8R7E6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLKISLIAP ILLLFSFFFA VESKCRTSCP LALASYYLEN GTTLSVINQN
60 70 80 90 100
LNSSIAPYDQ INFDPILRYN SNIKDKDRIQ MGSRVLVPFP CECQPGDFLG
110 120 130 140 150
HNFSYSVRQE DTYERVAISN YANLTTMESL QARNPFPATN IPLSATLNVL
160 170 180 190 200
VNCSCGDESV SKDFGLFVTY PLRPEDSLSS IARSSGVSAD ILQRYNPGVN
210 220 230 240 250
FNSGNGIVYV PGRDPNGAFP PFKSSKQDGV GAGVIAGIVI GVIVALLLIL
260 270 280 290 300
FIVYYAYRKN KSKGDSFSSS IPLSTKADHA SSTSLQSGGL GGAGVSPGIA
310 320 330 340 350
AISVDKSVEF SLEELAKATD NFNLSFKIGQ GGFGAVYYAE LRGEKAAIKK
360 370 380 390 400
MDMEASKQFL AELKVLTRVH HVNLVRLIGY CVEGSLFLVY EYVENGNLGQ
410 420 430 440 450
HLHGSGREPL PWTKRVQIAL DSARGLEYIH EHTVPVYVHR DIKSANILID
460 470 480 490 500
QKFRAKVADF GLTKLTEVGG SATRGAMGTF GYMAPETVYG EVSAKVDVYA
510 520 530 540 550
FGVVLYELIS AKGAVVKMTE AVGEFRGLVG VFEESFKETD KEEALRKIID
560 570 580 590 600
PRLGDSYPFD SVYKMAELGK ACTQENAQLR PSMRYIVVAL STLFSSTGNW
610
DVGNFQNEDL VSLMSGR
Length:617
Mass (Da):67,315
Last modified:January 15, 2008 - v1
Checksum:iCF5019DB428CFE1E
GO

Sequence cautioni

The sequence BAB02358 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB367524 mRNA. Translation: BAF92788.1.
AB019232 Genomic DNA. Translation: BAB02358.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE76532.1.
RefSeqiNP_566689.2. NM_113058.4.
UniGeneiAt.5663.

Genome annotation databases

EnsemblPlantsiAT3G21630.1; AT3G21630.1; AT3G21630.
GeneIDi821717.
GrameneiAT3G21630.1; AT3G21630.1; AT3G21630.
KEGGiath:AT3G21630.

Similar proteinsi

Entry informationi

Entry nameiCERK1_ARATH
AccessioniPrimary (citable) accession number: A8R7E6
Secondary accession number(s): Q9LVE3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 9, 2013
Last sequence update: January 15, 2008
Last modified: November 22, 2017
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families