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A8QL58

- OXLA_NAJAT

UniProt

A8QL58 - OXLA_NAJAT

Protein

L-amino-acid oxidase

Gene
N/A
Organism
Naja atra (Chinese cobra)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 22 (01 Oct 2014)
      Sequence version 1 (15 Jan 2008)
      Previous versions | rss
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    Functioni

    Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids, thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as hemorrhage, hemolysis, edema, apoptosis of vascular endothelial cells or tumor cell lines, antibacterial and antiparasitic activities By similarity. This protein induces platelet aggregation by both hydrogen peroxide production and binding to platelet membrane proteins (that would enhance the sensitivity of platelets to hydrogen peroxide). Effects of snake L-amino oxidases on platelets are controversial, since they either induce aggregation or inhibit agonist-induced aggregation. These different effects are probably due to different experimental conditions.By similarity1 Publication

    Catalytic activityi

    An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

    Cofactori

    FAD.By similarity

    Kineticsi

      Vmax=38.4 µmol/min/mg enzyme1 Publication

      Sites

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Binding sitei90 – 901FADBy similarity
      Binding sitei109 – 1091SubstrateBy similarity
      Binding sitei280 – 2801FAD; via amide nitrogen and carbonyl oxygenBy similarity
      Binding sitei389 – 3891SubstrateBy similarity

      Regions

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Nucleotide bindingi62 – 632FADBy similarity
      Nucleotide bindingi82 – 832FADBy similarity
      Nucleotide bindingi106 – 1094FADBy similarity

      GO - Molecular functioni

      1. L-amino-acid oxidase activity Source: UniProtKB-EC

      GO - Biological processi

      1. apoptotic process Source: UniProtKB-KW
      2. defense response to bacterium Source: UniProtKB-KW
      3. hemolysis in other organism Source: UniProtKB-KW

      Keywords - Molecular functioni

      Antibiotic, Antimicrobial, Hemostasis impairing toxin, Oxidoreductase, Platelet aggregation activating toxin, Toxin

      Keywords - Biological processi

      Apoptosis, Cytolysis, Hemolysis

      Keywords - Ligandi

      FAD, Flavoprotein

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      L-amino-acid oxidase (EC:1.4.3.2)
      Short name:
      LAAO
      Short name:
      LAO
      Short name:
      NA-LAAO
      OrganismiNaja atra (Chinese cobra)
      Taxonomic identifieri8656 [NCBI]
      Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

      Subcellular locationi

      GO - Cellular componenti

      1. extracellular region Source: UniProtKB-SubCell

      Keywords - Cellular componenti

      Secreted

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Signal peptidei1 – 19191 PublicationAdd
      BLAST
      Chaini20 – ›449›430L-amino-acid oxidasePRO_0000412603Add
      BLAST

      Amino acid modifications

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Disulfide bondi29 ↔ 192By similarity
      Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis
      Disulfide bondi348 ↔ 429By similarity
      Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence Analysis

      Post-translational modificationi

      N-glycosylated.By similarity

      Keywords - PTMi

      Disulfide bond, Glycoprotein

      Expressioni

      Tissue specificityi

      Expressed by the venom gland.

      Interactioni

      Subunit structurei

      Homodimer; non-covalently linked.By similarity

      Structurei

      3D structure databases

      ProteinModelPortaliA8QL58.
      ModBaseiSearch...
      MobiDBiSearch...

      Family & Domainsi

      Sequence similaritiesi

      Keywords - Domaini

      Signal

      Phylogenomic databases

      HOVERGENiHBG005729.

      Family and domain databases

      InterProiIPR002937. Amino_oxidase.
      [Graphical view]
      PfamiPF01593. Amino_oxidase. 1 hit.
      [Graphical view]

      Sequencei

      Sequence statusi: Fragment.

      Sequence processingi: The displayed sequence is further processed into a mature form.

      A8QL58-1 [UniParc]FASTAAdd to Basket

      « Hide

      MNVLFIFSLL FLAALESCAD DRRSPLEECF QQNDYEEILE IARNGLKKTS    50
      NPKHVVVVGA GMAGLSAAYV LAGAGHKVTL LEASERVGGR VITYHNDREG 100
      WYVNMGPMRL PERHRIVREY IRKFGLKLNE FFQENENAWY YINNIRKRVW 150
      EVKKDPSLLK YPVKPSEEGK SASQLYQEPL RKVIEELKRT NCSYILNKYD 200
      SYSTKEYLIK EGNLSRGAVD MIGDLLNEDS SYHLSFMESL KSDALFSYEK 250
      RFDEIVGGFD QLPISMYQAI AEMVHLNARV IKIQYDAEKV RVTYQTPAKT 300
      FVTADYVIVC STSRAARRIY FEPPLPPKKA HALRSIHYRS ATKIFLTCSK 350
      KFWEADGIHG GKSTTDLPSR FIHYPNHNFT SGIGVIMAYV LADDSDFFQA 400
      LDTKTCADIV INDLSLIHDL PKREIQALCY PSIKKWNLDK YTMGSITSF 449
      Length:449
      Mass (Da):51,439
      Last modified:January 15, 2008 - v1
      Checksum:i905AD5DFB3D08362
      GO

      Experimental Info

      Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
      Non-terminal residuei449 – 4491

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      EF080839 mRNA. Translation: ABN72546.1.

      Cross-referencesi

      Sequence databases

      Select the link destinations:
      EMBL
      GenBank
      DDBJ
      Links Updated
      EF080839 mRNA. Translation: ABN72546.1 .

      3D structure databases

      ProteinModelPortali A8QL58.
      ModBasei Search...
      MobiDBi Search...

      Protocols and materials databases

      Structural Biology Knowledgebase Search...

      Phylogenomic databases

      HOVERGENi HBG005729.

      Family and domain databases

      InterProi IPR002937. Amino_oxidase.
      [Graphical view ]
      Pfami PF01593. Amino_oxidase. 1 hit.
      [Graphical view ]
      ProtoNeti Search...

      Publicationsi

      1. "Molecular characterization of L-amino acid oxidase from king cobra venom."
        Jin Y., Lee W.-H., Zeng L., Zhang Y.
        Toxicon 50:479-489(2007) [PubMed] [Europe PMC] [Abstract]
        Cited for: NUCLEOTIDE SEQUENCE [MRNA].
        Tissue: Venom gland.
      2. "L-amino acid oxidase from Naja atra venom activates and binds to human platelets."
        Li R., Zhu S., Wu J., Wang W., Lu Q., Clemetson K.J.
        Acta Biochim. Biophys. Sin. 40:19-26(2008) [PubMed] [Europe PMC] [Abstract]
        Cited for: PROTEIN SEQUENCE OF 20-29, FUNCTION IN PLATELET AGGREGATION, BIOPHYSICOCHEMICAL PROPERTIES.
        Tissue: Venom.

      Entry informationi

      Entry nameiOXLA_NAJAT
      AccessioniPrimary (citable) accession number: A8QL58
      Entry historyi
      Integrated into UniProtKB/Swiss-Prot: September 21, 2011
      Last sequence update: January 15, 2008
      Last modified: October 1, 2014
      This is version 22 of the entry and version 1 of the sequence. [Complete history]
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programAnimal Toxin Annotation Program
      Annotation programChordata Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      Direct protein sequencing

      Documents

      1. SIMILARITY comments
        Index of protein domains and families

      External Data

      Dasty 3