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A8QL58

- OXLA_NAJAT

UniProt

A8QL58 - OXLA_NAJAT

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Protein

L-amino-acid oxidase

Gene
N/A
Organism
Naja atra (Chinese cobra)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids, thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as hemorrhage, hemolysis, edema, apoptosis of vascular endothelial cells or tumor cell lines, antibacterial and antiparasitic activities (By similarity). This protein induces platelet aggregation by both hydrogen peroxide production and binding to platelet membrane proteins (that would enhance the sensitivity of platelets to hydrogen peroxide). Effects of snake L-amino oxidases on platelets are controversial, since they either induce aggregation or inhibit agonist-induced aggregation. These different effects are probably due to different experimental conditions.By similarity1 Publication

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactori

FAD.By similarity

Kineticsi

    Vmax=38.4 µmol/min/mg enzyme1 Publication

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei90 – 901FADBy similarity
    Binding sitei109 – 1091SubstrateBy similarity
    Binding sitei280 – 2801FAD; via amide nitrogen and carbonyl oxygenBy similarity
    Binding sitei389 – 3891SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi62 – 632FADBy similarity
    Nucleotide bindingi82 – 832FADBy similarity
    Nucleotide bindingi106 – 1094FADBy similarity

    GO - Molecular functioni

    1. L-amino-acid oxidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. apoptotic process Source: UniProtKB-KW
    2. defense response to bacterium Source: UniProtKB-KW
    3. hemolysis in other organism Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Molecular functioni

    Antibiotic, Antimicrobial, Hemostasis impairing toxin, Oxidoreductase, Platelet aggregation activating toxin, Toxin

    Keywords - Biological processi

    Apoptosis, Cytolysis, Hemolysis

    Keywords - Ligandi

    FAD, Flavoprotein

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-amino-acid oxidase (EC:1.4.3.2)
    Short name:
    LAAO
    Short name:
    LAO
    Short name:
    NA-LAAO
    OrganismiNaja atra (Chinese cobra)
    Taxonomic identifieri8656 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-KW
    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 19191 PublicationAdd
    BLAST
    Chaini20 – ›449›430L-amino-acid oxidasePRO_0000412603Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi29 ↔ 192By similarity
    Glycosylationi191 – 1911N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi348 ↔ 429By similarity
    Glycosylationi378 – 3781N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Interactioni

    Subunit structurei

    Homodimer; non-covalently linked.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliA8QL58.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG005729.

    Family and domain databases

    InterProiIPR002937. Amino_oxidase.
    [Graphical view]
    PfamiPF01593. Amino_oxidase. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A8QL58-1 [UniParc]FASTAAdd to Basket

    « Hide

            10         20         30         40         50
    MNVLFIFSLL FLAALESCAD DRRSPLEECF QQNDYEEILE IARNGLKKTS
    60 70 80 90 100
    NPKHVVVVGA GMAGLSAAYV LAGAGHKVTL LEASERVGGR VITYHNDREG
    110 120 130 140 150
    WYVNMGPMRL PERHRIVREY IRKFGLKLNE FFQENENAWY YINNIRKRVW
    160 170 180 190 200
    EVKKDPSLLK YPVKPSEEGK SASQLYQEPL RKVIEELKRT NCSYILNKYD
    210 220 230 240 250
    SYSTKEYLIK EGNLSRGAVD MIGDLLNEDS SYHLSFMESL KSDALFSYEK
    260 270 280 290 300
    RFDEIVGGFD QLPISMYQAI AEMVHLNARV IKIQYDAEKV RVTYQTPAKT
    310 320 330 340 350
    FVTADYVIVC STSRAARRIY FEPPLPPKKA HALRSIHYRS ATKIFLTCSK
    360 370 380 390 400
    KFWEADGIHG GKSTTDLPSR FIHYPNHNFT SGIGVIMAYV LADDSDFFQA
    410 420 430 440
    LDTKTCADIV INDLSLIHDL PKREIQALCY PSIKKWNLDK YTMGSITSF
    Length:449
    Mass (Da):51,439
    Last modified:January 15, 2008 - v1
    Checksum:i905AD5DFB3D08362
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei449 – 4491

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF080839 mRNA. Translation: ABN72546.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EF080839 mRNA. Translation: ABN72546.1 .

    3D structure databases

    ProteinModelPortali A8QL58.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Phylogenomic databases

    HOVERGENi HBG005729.

    Family and domain databases

    InterProi IPR002937. Amino_oxidase.
    [Graphical view ]
    Pfami PF01593. Amino_oxidase. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of L-amino acid oxidase from king cobra venom."
      Jin Y., Lee W.-H., Zeng L., Zhang Y.
      Toxicon 50:479-489(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Venom gland.
    2. "L-amino acid oxidase from Naja atra venom activates and binds to human platelets."
      Li R., Zhu S., Wu J., Wang W., Lu Q., Clemetson K.J.
      Acta Biochim. Biophys. Sin. 40:19-26(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 20-29, FUNCTION IN PLATELET AGGREGATION, BIOPHYSICOCHEMICAL PROPERTIES.
      Tissue: Venom.

    Entry informationi

    Entry nameiOXLA_NAJAT
    AccessioniPrimary (citable) accession number: A8QL58
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 21, 2011
    Last sequence update: January 15, 2008
    Last modified: October 29, 2014
    This is version 23 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programAnimal Toxin Annotation Program
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3