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Protein

L-amino-acid oxidase

Gene
N/A
Organism
Naja atra (Chinese cobra)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes an oxidative deamination of predominantly hydrophobic and aromatic L-amino acids, thus producing hydrogen peroxide that may contribute to the diverse toxic effects of this enzyme. Exhibits diverse biological activities, such as hemorrhage, hemolysis, edema, apoptosis of vascular endothelial cells or tumor cell lines, antibacterial and antiparasitic activities (By similarity). This protein induces platelet aggregation by both hydrogen peroxide production and binding to platelet membrane proteins (that would enhance the sensitivity of platelets to hydrogen peroxide). Effects of snake L-amino oxidases on platelets are controversial, since they either induce aggregation or inhibit agonist-induced aggregation. These different effects are probably due to different experimental conditions.By similarity1 Publication

Catalytic activityi

An L-amino acid + H2O + O2 = a 2-oxo acid + NH3 + H2O2.

Cofactori

FADBy similarity

Kineticsi

    1. Vmax=38.4 µmol/min/mg enzyme1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei90FADBy similarity1
    Binding sitei109SubstrateBy similarity1
    Binding sitei280FAD; via amide nitrogen and carbonyl oxygenBy similarity1
    Binding sitei389SubstrateBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi62 – 63FADBy similarity2
    Nucleotide bindingi82 – 83FADBy similarity2
    Nucleotide bindingi106 – 109FADBy similarity4

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionAntibiotic, Antimicrobial, Hemostasis impairing toxin, Oxidoreductase, Platelet aggregation activating toxin, Toxin
    Biological processApoptosis, Cytolysis, Hemolysis
    LigandFAD, Flavoprotein

    Enzyme and pathway databases

    BRENDAi1.4.3.2. 3558.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    L-amino-acid oxidase (EC:1.4.3.2)
    Short name:
    LAAO
    Short name:
    LAO
    Short name:
    NA-LAAO
    OrganismiNaja atra (Chinese cobra)
    Taxonomic identifieri8656 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaElapidaeElapinaeNaja

    Subcellular locationi

    GO - Cellular componenti

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Signal peptidei1 – 191 PublicationAdd BLAST19
    ChainiPRO_000041260320 – ›449L-amino-acid oxidaseAdd BLAST›430

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Disulfide bondi29 ↔ 192By similarity
    Glycosylationi191N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi348 ↔ 429By similarity
    Glycosylationi378N-linked (GlcNAc...) asparagineSequence analysis1

    Post-translational modificationi

    N-glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed by the venom gland.

    Interactioni

    Subunit structurei

    Homodimer; non-covalently linked.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliA8QL58.
    SMRiA8QL58.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Signal

    Phylogenomic databases

    HOVERGENiHBG005729.

    Family and domain databases

    InterProiView protein in InterPro
    IPR002937. Amino_oxidase.
    IPR023753. FAD/NAD-binding_dom.
    PfamiView protein in Pfam
    PF01593. Amino_oxidase. 1 hit.
    SUPFAMiSSF51905. SSF51905. 1 hit.

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    A8QL58-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MNVLFIFSLL FLAALESCAD DRRSPLEECF QQNDYEEILE IARNGLKKTS
    60 70 80 90 100
    NPKHVVVVGA GMAGLSAAYV LAGAGHKVTL LEASERVGGR VITYHNDREG
    110 120 130 140 150
    WYVNMGPMRL PERHRIVREY IRKFGLKLNE FFQENENAWY YINNIRKRVW
    160 170 180 190 200
    EVKKDPSLLK YPVKPSEEGK SASQLYQEPL RKVIEELKRT NCSYILNKYD
    210 220 230 240 250
    SYSTKEYLIK EGNLSRGAVD MIGDLLNEDS SYHLSFMESL KSDALFSYEK
    260 270 280 290 300
    RFDEIVGGFD QLPISMYQAI AEMVHLNARV IKIQYDAEKV RVTYQTPAKT
    310 320 330 340 350
    FVTADYVIVC STSRAARRIY FEPPLPPKKA HALRSIHYRS ATKIFLTCSK
    360 370 380 390 400
    KFWEADGIHG GKSTTDLPSR FIHYPNHNFT SGIGVIMAYV LADDSDFFQA
    410 420 430 440
    LDTKTCADIV INDLSLIHDL PKREIQALCY PSIKKWNLDK YTMGSITSF
    Length:449
    Mass (Da):51,439
    Last modified:January 15, 2008 - v1
    Checksum:i905AD5DFB3D08362
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Non-terminal residuei4491

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    EF080839 mRNA. Translation: ABN72546.1.

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

    Entry informationi

    Entry nameiOXLA_NAJAT
    AccessioniPrimary (citable) accession number: A8QL58
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 21, 2011
    Last sequence update: January 15, 2008
    Last modified: May 10, 2017
    This is version 30 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    Annotation programAnimal Toxin Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. SIMILARITY comments
      Index of protein domains and families