Reviewed, UniProtKB/Swiss-Prot A8QFF6 (SPAST_BRUMA)

Names and origin

Protein names

Recommended name:
    Probable spastin homolog Bm1_53365
    EC=3.6.4.3

Gene names

ORF Names:

Bm1_53365

Organism

Brugia malayi (Filarial nematode worm)

Taxonomic identifier

6279 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Spirurida › Filarioidea › Onchocercidae › Brugia

Protein attributes

Sequence length	454 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Severs microtubules, probably in an ATP-dependent fashion By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Homohexamer. The homohexamer is stabilized by ATP-binding. The homohexamer may adopt a ring conformation through which microtubules pass prior to being severed. Interacts with microtubules By similarity.
Subcellular location	Cytoplasm › cytoskeleton By similarity. Cytoplasm › perinuclear region By similarity.
Sequence similarities	Belongs to the AAA ATPase family. Spastin subfamily.
Caution	Lacks the conserved MIT domain, which is one of the features of the spastin family.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton Microtubule
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Gene Ontology (GO)
Biological process	protein hexamerization Inferred from sequence or structural similarity. Source: UniProtKB protein homooligomerization Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	microtubule Inferred from electronic annotation. Source: UniProtKB-KW perinuclear region of cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW microtubule-severing ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 454	454	Probable spastin homolog Bm1_53365		PRO_0000367130
Regions
	Nucleotide binding	218 – 225	8	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	A8QFF6-1 [UniParc]. Last modified January 15, 2008. Version 1. Checksum: CC1366536E4FB80A	FASTA	454	51,150
	10 20 30 40 50 60 MLHPQKLEQQ NYETFNKAYL KSKQLVTEGV SIDEISSNND EQRKRIAMEK YRMGIEYFEK 70 80 90 100 110 120 ALKISPDKVY PEKRSEVITH REAMKRNLEA TKGRLSDLEK MFPSKGNRNL QHRPVQFVSP 130 140 150 160 170 180 SISKPQTAQL SSRPISSEKK NINYSNARTR SNLLKGVDDK FGGPLLNEIL NQDDVKMSDI 190 200 210 220 230 240 IGAETAKRAL EETVILPTVN PSLFSGLRQP AQGILLFGPP GNGKTLLARA VAGECGSTMF 250 260 270 280 290 300 LNVSAASLTS KWVGDAEKIV RALFQIARNG QPTIIFIDEI DSILCERNEK ETEVSRRMKT 310 320 330 340 350 360 EFLIQMDGML SSKDDRLLVI GATNRPEELD SAILRRFPKR ILIDVPNAAA RLKLIMSLLE 370 380 390 400 410 420 KTKTSFDLGL TQRQILAEWT HGYSNSDLVA LCREAAMVPI RDLSRKDIKN LVSTELRPIT 430 440 450 LRDFEIAMKA IKPSTNERML QKLRKYAATA GQSD

References

[1]

"Draft genome of the filarial nematode parasite Brugia malayi." Ghedin E., Wang S., Spiro D., Caler E., Zhao Q., Crabtree J., Allen J.E., Delcher A.L., Guiliano D.B., Miranda-Saavedra D., Angiuoli S.V., Creasy T., Amedeo P., Haas B., El-Sayed N.M., Wortman J.R., Feldblyum T., Tallon L. , Schatz M., Shumway M., Koo H., Salzberg S.L., Schobel S., Pertea M., Pop M., White O., Barton G.J., Carlow C.K.S., Crawford M.J., Daub J., Dimmic M.W., Estes C.F., Foster J.M., Ganatra M., Gregory W.F., Johnson N.M., Jin J., Komuniecki R., Korf I., Kumar S., Laney S., Li B.-W., Li W., Lindblom T.H., Lustigman S., Ma D., Maina C.V., Martin D.M.A., McCarter J.P., McReynolds L., Mitreva M., Nutman T.B., Parkinson J., Peregrin-Alvarez J.M., Poole C., Ren Q., Saunders L., Sluder A.E., Smith K., Stanke M., Unnasch T.R., Ware J., Wei A.D., Weil G., Williams D.J., Zhang Y., Williams S.A., Fraser-Liggett C., Slatko B., Blaxter M.L., Scott A.L. Science 317:1756-1760(2007) [PubMed: 17885136] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	DS239470 Genomic DNA. Translation: EDP29011.1.
RefSeq	XP_001902141.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	6105557.
KEGG	bmy:Bm1_53365.
Organism-specific databases
CTD	6105557.
Family and domain databases
InterPro	IPR003593. ATPase_AAA+_core. IPR003959. ATPase_AAA_core. IPR003960. ATPase_AAA_CS. [Graphical view]
Pfam	PF00004. AAA. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
PROSITE	PS00674. AAA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SPAST_BRUMA

Accession

Primary (citable) accession number: A8QFF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 24, 2009
Last sequence update:	January 15, 2008
Last modified:	October 13, 2009
This is version 17 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)